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pNK3071

Plasmid

#219755

Purpose

MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH

Expression

Plant

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pJL1-sfGFP

Plasmid

#102634

Purpose

In vitro expression of sfGFP from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

sfGFP

Tags

strep tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pJL1-mCherry

Plasmid

#102629

Purpose

In vitro expression of mCherry from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

mCherry

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pJL1-dTomato

Plasmid

#102631

Purpose

In vitro expression of tdTomato from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

tdTomato

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcdna6.2- myc ULK1 wt

Plasmid

#27629

Depositor

Reuben Shaw

Article

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Insert

mouse myc ULK1 (Myc Mouse)

Tags

myc

Expression

Mammalian

Mutation

Initial M from ULK1 removed. Silent mutation at A…

Available Since

Feb. 18, 2011

Availability

Academic Institutions and Nonprofits only
pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE

Plasmid

#162380

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expression

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

jGCaMP8s

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

S26M F286Y Q315H

Promoter

CAG

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pMXS-mitoGshF-blasticidin

Plasmid

#210314

Purpose

Expresses mitochondrially targeted, humanized Streptococcus thermophilus GshF in mammalian cells

Depositor

Kivanc Birsoy

Article

Wang et al Nature. 2021 Nov;599(7883):136-140. doi:

Insert

Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)

Use

Retroviral

Tags

FLAG peptide, Homo sapiens ACO2 mitochondrial tar…

Mutation

Codon optimized for expression in human cells

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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PCR4-Shh::lacZ-H11

Plasmid

#139098

Purpose

enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)

Depositor

Len Pennacchio

Article

Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

Shh

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-CMVtight-PLXNB2 iOE

Plasmid

#176849

Purpose

lentiviral vector for doxycycline-inducible PLXNB2 overexpression

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

hPLXNB2 (PLXNB2 Human)

Use

Lentiviral

Mutation

full length PLXNB2

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pSicoR-eIF1a-GFP-RPL10a

Plasmid

#159890

Purpose

Expresses GFP-RPL10a for ribosomal immunoprecipitation

Depositor

James Ellis

Article

Rodrigues et al Cell Rep. 2020 Mar 24;30(12):4179-4196.e

Insert

RPL10a (RPL10A Human)

Use

Lentiviral

Tags

GFP

Promoter

eIF1a

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-FLEX-jGCaMP8m-WPRE

Plasmid

#162378

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8m

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

A25G F286Y

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pJL1-mOrange

Plasmid

#102632

Purpose

In vitro expression of mOrange from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

mOrange

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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PCR4-Hsp68::lacZ-H11

Plasmid

#139099

Purpose

enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Hsp68 minimal promoter)

Depositor

Len Pennacchio

Article

Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

Hsp68

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF423

Plasmid

#88083

Purpose

Donor Vector containing ZNF423 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF423 (ZNF423 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-FLEX-jGCaMP8s-WPRE

Plasmid

#162377

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

jGCaMP8s

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

S26M F286Y Q315H

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pUltra-Hot-PC

Plasmid

#184466

Purpose

Lentiviral vector for bi-cistronic expression of mCherry and PC (seperated by P2A)

Depositor

Gerardo Ferbeyre

Article

Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

Insert

Pyruvate Carboxylase (PC Human)

Use

Lentiviral

Expression

Mammalian

Promoter

UbC

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pNK497

Plasmid

#219754

Purpose

MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH

Expression

Plant

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-FLEX-jGCaMP8f-WPRE

Plasmid

#162379

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8f

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

Q315L

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pJL1-mRFP1

Plasmid

#102630

Purpose

In vitro expression of mRFP1 from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

mRFP1

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-PLXNB2-shRNA1

Plasmid

#98399

Purpose

Lentivirus for expression of shRNA1 against human PLEXIN-B2 (Dox-inducible)

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

Plexin-B2 (PLXNB2 Human)

Use

Lentiviral

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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