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pSTAP-seq_fly-4xUAS

Plasmid

#125149

Purpose

Vector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in fly cells

Depositor

Alexander Stark

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Insert

4 x upstream activating sequence (UAS)

Use

Stap-seq screening vector

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pKD286

Plasmid

#125074

Purpose

Expression of SO_4622 under Ptac

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

SO_4622 (SO_4622 )
lacI (lacI )

Use

Synthetic Biology

Expression

Bacterial

Mutation

S286L- Please see depositor comments below

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pKD276

Plasmid

#125069

Purpose

Expression of SO_0621 under Ptac

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

SO_0621 (SO_0621 )
lacI (lacI )

Use

Synthetic Biology

Expression

Bacterial

Mutation

S286L- Please see depositor comments below

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pKD250.2

Plasmid

#125077

Purpose

Expression of torR under PLtetO-1, output promoter PtorCAD129

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

torR (torR )
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL251 GG sgRNA

Plasmid

#126436

Purpose

To target a protospacer with GG at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with GG at cut site

Expression

Mammalian

Promoter

human U6

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL510 CA sgRNA

Plasmid

#126430

Purpose

To target a protospacer with CA at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with CA at cut site

Expression

Mammalian

Promoter

human U6

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL401 GC sgRNA

Plasmid

#126435

Purpose

To target a protospacer with GC at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with GC at cut site

Expression

Mammalian

Promoter

human U6

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL071 AG sgRNA

Plasmid

#126428

Purpose

To target a protospacer with AG at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with AG at cut site

Expression

Mammalian

Promoter

human U6

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL402 AC sgRNA

Plasmid

#126427

Purpose

To target a protospacer with AC at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with AC at cut site

Expression

Mammalian

Promoter

human U6

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL507 GA sgRNA

Plasmid

#126434

Purpose

To target a protospacer with GA at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with GA at cut site

Expression

Mammalian

Promoter

human U6

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL512 CG sgRNA

Plasmid

#126432

Purpose

To target a protospacer with CG at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with CG at cut site

Expression

Mammalian

Promoter

human U6

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL063 GT sgRNA

Plasmid

#126437

Purpose

To target a protospacer with GT at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with GT at cut site

Expression

Mammalian

Promoter

human U6

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL516 CT sgRNA

Plasmid

#126433

Purpose

To target a protospacer with CT at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with CT at cut site

Expression

Mammalian

Promoter

human U6

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL515 AA sgRNA

Plasmid

#126426

Purpose

To target a protospacer with AA at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with AA at cut site

Expression

Mammalian

Promoter

human U6

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL509 AT sgRNA

Plasmid

#126429

Purpose

To target a protospacer with AT at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with AT at cut site

Expression

Mammalian

Promoter

human U6

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL511 CC sgRNA

Plasmid

#126431

Purpose

To target a protospacer with CC at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with CC at cut site

Expression

Mammalian

Promoter

human U6

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL508 TA sgRNA

Plasmid

#126438

Purpose

To target a protospacer with TA at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with TA at cut site

Expression

Mammalian

Promoter

human U6

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL250 TC sgRNA

Plasmid

#126439

Purpose

To target a protospacer with TC at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with TC at cut site

Expression

Mammalian

Promoter

human U6

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL514 TG sgRNA

Plasmid

#126440

Purpose

To target a protospacer with TG at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with TG at cut site

Expression

Mammalian

Promoter

human U6

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL513 TT sgRNA

Plasmid

#126441

Purpose

To target a protospacer with TT at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with TT at cut site

Expression

Mammalian

Promoter

human U6

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge