We narrowed to 25,210 results for: promoter

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AAV-TRE-scFVtet2_bGHpA

Plasmid

#177358

Purpose

AAV expression of scFV-fused catalytic domain of TET2 from doxycycine-inducible promoter for targeted DNA methylation editing

Depositor

Naoki Yamamoto

Article

Yamamoto et al Research Square [Preprint] 2021. doi: 10

Insert

Tet Methylcytosine Dioxygenase 2 (TET2 Human)

Use

AAV

Tags

myc and scFV

Expression

Mammalian

Mutation

Catalytic domains of human TET2 (1129–1936 and 14…

Promoter

Tetracycline-dependent promoter

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD28-mTFP1

Plasmid

#223638

Purpose

Lentiviral expression of human CD28 with mTFP1 tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

mTFP1

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-AP-SpyCatcher

Plasmid

#223636

Purpose

Protein purification of SpyCatcher003 (S49C) with 10xHis and AP fragment of split APEX2 tags from E. coli

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Tags

His10, AP fragment of split APEX2 tag

Expression

Bacterial

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-ALFA-CD28

Plasmid

#223630

Purpose

Lentiviral expression of human CD28 with ALFA tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

ALFA tag

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD28(Y209F)

Plasmid

#223627

Purpose

Lentiviral expression of human CD28 (Y209F) in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Mutation

Y209F

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD28(Y191F)

Plasmid

#223625

Purpose

Lentiviral expression of human CD28 (Y191F) in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Mutation

Y191F

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-Spy-CD28

Plasmid

#223622

Purpose

Lentiviral expression of human CD28 with SpyTag003 in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

Spytag003

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD28-LgBiT

Plasmid

#223616

Purpose

Lentiviral expression of human CD28 with LgBiT tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

LgBiT

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD28-CLIP

Plasmid

#223610

Purpose

Lentiviral expression of human CD28 with CLIP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

CLIP

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CLIP-CD28

Plasmid

#223600

Purpose

Lentiviral expression of human CD28 with CLIP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

CLIP

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pSB278 - pL0_pT16H2 (pro + 5U)

Plasmid

#203889

Purpose

T16H2 promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

T16H2 promoter and 5'UTR

Expression

Plant

Mutation

Mutation of BpiI or BsaI cut site for MoClo domes…

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUN1224 - pL0_pT3R (pro + 5U)

Plasmid

#203892

Purpose

T3R promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

T3R promoter and 5'UTR

Expression

Plant

Mutation

Mutation of BpiI or BsaI cut site for MoClo domes…

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUN1215 - pL0_pDAT (pro + 5U)

Plasmid

#203895

Purpose

DAT promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

DAT promoter and 5'UTR

Expression

Plant

Mutation

Mutation of BpiI or BsaI cut site for MoClo domes…

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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NSP4-EGFP

Plasmid

#210378

Purpose

Expresses SARS-CoV-2 NSP4 fused with EGFP in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

NSP4 (ORF1ab SARS-CoV-2)

Tags

EGFP

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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NSP2-Flag

Plasmid

#210344

Purpose

Expresses SARS-CoV-2 NSP2 in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP2 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)

Tags

Flag

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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Human USP47

Plasmid

#217624

Purpose

Mammalian expression of USP47

Depositor

Ethan Lee

Article

Kassel et al Sci Signal. 2023 Feb 7;16(771):eabn8372.

Insert

USP47 (USP47 Human)

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPR-cBEST-v2-SF14P

Plasmid

#209448

Purpose

improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the SF14P promoter

Depositor

Tilmann Weber

Article

Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

Insert

Codon optimized APOBEC1-nCas9-UGI fusion protein

Use

CRISPR and Synthetic Biology

Promoter

PtipA; sgRNA expression from SF14P promoter

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPR-cBEST-v2-kasOP*

Plasmid

#209447

Purpose

improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the kasOP* promoter

Depositor

Tilmann Weber

Article

Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

Insert

Codon optimized APOBEC1-nCas9-UGI fusion protein

Use

CRISPR and Synthetic Biology

Promoter

PtipA; sgRNA expression from kasOP* promoter

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-Doc2β C2AB (G361C)

Plasmid

#213631

Purpose

Bacterial expression of SUMO-tagged rat Doc2β C2AB (G361C)

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

Doc2β C2AB (G361C) (Doc2b Rat)

Tags

SUMO

Expression

Bacterial

Mutation

G361C, all original cysteines mutated to alanines

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-3xFLAG-bII spectrin (repeats 13-15)

Plasmid

#213729

Purpose

Mammalian cell expression vector for overexpression of bII spectrin (repeats 13-15) in NIH/3T3 cells.

Depositor

Chaolin Zhang

Article

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

bII spectrin (repeats 13-15) (Spnb3 Mouse)

Expression

Mammalian

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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