We narrowed to 9,647 results for: bli

1. pFP.R401

   Plasmid

   #138222

   Purpose

   Expresses the split mCherry reporter interrupted by the TerThyX artificially mini-intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(IGC).TerThyX*.(SFD)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   P(araBAD) and iPC

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFP.R360

   Plasmid

   #138230

   Purpose

   Expresses the split mCherry reporter interrupted by the Ama-Ter artificially mini-intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(HGK).Ama-Ter*.(SEL)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   P(araBAD) and iPC

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME-hygro-SNAP-FLAG-CD59

   Plasmid

   #50379

   Purpose

   Express SNAP and FLAG-tagged human CD59, a GPI-AP in mammalian cells

   Depositor

   Reika Watanabe

   Insert

   SNAP-FLAG-tagged CD59 (CD59 Human)
   

   Tags

   FLAG and SNAP

   Expression

   Mammalian

   Promoter

   SR alpha

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

4. SMARCA4

   Plasmid

   #39116

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   SMARCA4 (SMARCA4 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pFP.R371

   Plasmid

   #138241

   Purpose

   Expresses the split mCherry reporter interrupted by the MP-M-DnaB artificially mini-intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(NGK).MP-M-DnaB*.(TEL)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   P(araBAD) and iPC

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-SpCas9-NG-P2A-mScarlet (MNW005)

   Plasmid

   #174140

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-mScarlet
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-mScarlet

   Expression

   Mammalian

   Mutation

   SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…

   Promoter

   CMV and T7

   Available Since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVRC8400-SARS-CoV-2-RBD-BA.4/5-AVI

   Plasmid

   #192114

   Purpose

   Expresses SARS-CoV-2 RBD protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tag

   Depositor

   Peter Kwong

   Insert

   SARS-CoV-2-RBD-BA.4/5 (S )
   

   Tags

   AVI, HRV3C, and scFc

   Expression

   Mammalian

   Mutation

   G339D, S371F, S373P, S375F, T376A, D405N, R408S, …

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQTEV-VPS28

   Plasmid

   #34803

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   vacuolar protein sorting 28 (yeast) (VPS28 Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   March 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSB700 HL1gR4

   Plasmid

   #124450

   Purpose

   Expresses gRNA HL1 gR4 in the pSB700 backbone

   Depositor

   George Church

   Article

   Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

   Insert

   HL1 gR4
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. wt HA-dynamin 1 pUHD10-3

    Plasmid

    #34688

    Depositor

    Sandra Schmid

    Article

    Damke et al J Cell Biol. 1995 Oct;131(1):69-80.

    Insert

    dynamin 1 (DNM1L Human)
    

    Tags

    HA (variant)

    Expression

    Mammalian

    Available Since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

11. HA-hDAGLb-V5

    Plasmid

    #87675

    Purpose

    Expresses human DAGLb protein with a HA tag inserted in the first extracellular loop, and intracellular V5 tag located on the C-terminal

    Depositor

    Pat Doherty

    Insert

    Human Diacylglycerol Lipase Beta (DAGLB Human)
    

    Tags

    HA and V5 tag

    Expression

    Mammalian

    Mutation

    Hemagglutinin (HA) peptide sequence (YPYDVPDYA) f…

    Promoter

    T7

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQTEV-TPT1

    Plasmid

    #31558

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    tumor protein, translationally-controlled 1 (TPT1 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6-SacB-Cas9-T2A-mCherry_(BbsI)

    Plasmid

    #117070

    Purpose

    Encodes Cas9, T2A-mCherry, and AmpR which creates a CRISPR system application with reliable positive selection

    Depositor

    Kim Failor

    Inserts

    Cas9
    SacB
    

    Use

    CRISPR

    Tags

    3x FLAG, NLS, and T2A-mCherry

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. 3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2 probasin_mTQ2_FlpO

    Plasmid

    #68405

    Purpose

    The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex8 and SMAD4 ex2. are expressed by the U6 promoter

    Depositor

    Jannik Elverløv-Jakobsen

    Inserts

    3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2
    FlpO
    

    Use

    CRISPR

    Tags

    mTurquoise2 (BFP)

    Expression

    Mammalian

    Promoter

    Synthetic Probasin ARRx2 and U6

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGAGmEos2-N1

    Plasmid

    #46372

    Depositor

    Suliana Manley

    Article

    Gunzenhauser et al Nano Lett. 2012 Sep 12;12(9):4705-10. do

    Insert

    Gag
    

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Rev independentHXB2 Gag sequence

    Promoter

    CMV

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUAS-kkv-R896K::NG

    Plasmid

    #138957

    Purpose

    Used to generate a transgene that will express an inactive fluorescent Chitin Synthase in Drosophila using Gal4

    Depositor

    Paul Adler

    Article

    Adler et al bioRxiv 2020

    Insert

    kkv-R896K::NG (kkv Fly)
    

    Tags

    Neon Green fused to C terminus of Kkv

    Expression

    Insect

    Mutation

    R896K

    Promoter

    UAS

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFUSE-rabbitFc1-IL2ss-VHH(B12)-His-Myc

    Plasmid

    #209837

    Purpose

    Plasmid coding for a nanobody against FGFR3 (mus musculus) with x6 HisTag, x3 myc, and Fabbit FC gamma 1

    Depositor

    Tim Brown

    Inserts

    IL2-ss
    VHH (B12 variant)
    Rabbit Fc Gamma 1
    

    Tags

    x3 MycTag and x6 HisTag

    Expression

    Mammalian

    Promoter

    hEF1-HTLV promoter

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-SYN-eGFP-RAB11

    Plasmid

    #203731

    Purpose

    AAV vector plasmid expressing human RAB11 fused to eGFP under the human synapsin (SYN) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Insert

    RAB11 (RAB11A Human)
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    Human synapsin promoter

    Available Since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. ATM_N_FS_v2_Donor

    Plasmid

    #214815

    Purpose

    N-terminal tagging of human ATM with 3xFLAG_Twin_Strep

    Depositor

    Yannick Doyon

    Insert

    3xFLAG_2xStrep for tagging ATM at the N-terminus (ATM Human, Synthetic)
    

    Use

    CRISPR

    Tags

    3xFLAG_2xStrep

    Expression

    Mammalian

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFP.R400

    Plasmid

    #138175

    Purpose

    Expresses the split mCherry reporter interrupted by the chimeric Npu/SspDnaE intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(GGK).NpuSsp*.(CWN)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    P(araBAD) and iPC

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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