We narrowed to 66,929 results for: nin

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Pepper-fused sgRNA

Plasmid

#217520

Purpose

Expresses Pepper-fused sgRNA in mammalian cells with U6 promoter

Depositor

Xing Li

Publication

Zhang et al Nat Commun. 2024 Jan 31;15(1):934. doi:

Insert

Pepper-fused sgRNA for spCas9

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLX307-VHL-3F-BirA

Plasmid

#220147

Purpose

Expresses VHL-BirA for VHL substrate identification by E-STUB

Depositor

William Sellers

Publication

Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

Insert

von Hippel-Lindau tumor suppressor (VHL Human)

Use

Lentiviral

Tags

3F-BirA

Expression

Mammalian

Mutation

codon optimized

Promoter

EF-1a

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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EGFP-N-Ezrin

Plasmid

#202684

Purpose

fluorescent protein tagged ezrin

Depositor

Gerry Hammond

Publication

Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Mus musculus EZR(1-586):EGFP (Ezr Mouse)

Expression

Mammalian

Available since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCW57.1-MCU-Flag-TetOFF

Plasmid

#185657

Purpose

Tet/dox-repressible expression of C-terminus Flag tagged human mitochondrial calcium uniporter

Depositor

Dipayan Chaudhuri

Publication

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

Mitochondrial calcium uniporter (MCU Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

pTight TRE

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pTol2CG2-QUAS5x:GFPNLS-SV40pA

Plasmid

#155122

Purpose

Tol2 vector containing QUAS5x reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Publication

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

QUAS5x:GFPNLS

Use

Zebrafish expression

Promoter

QUAS5x-E1b

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-EA-BE4max-NG-BlastR

Plasmid

#152992

Purpose

High purity C-to-T base editor

Depositor

David Liu

Publication

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BE4 H47E+S48A-nSpCas9-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pRRL-mU6-mScarletGO-P2A-Blas-PGK-Neo

Plasmid

#136904

Purpose

Lentivirus for base editing activatable mScarletI expression in mammalian cells. All-in-one vector with sgmScarletGO.

Depositor

Lukas Dow

Publication

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

mScarletGo-p2a-Blas

Use

Lentiviral

Mutation

ATG>ACG

Promoter

SFFV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

Plasmid

#125228

Purpose

Expresses SPARK2 NanoLuc-βarrestin2-TEVp in mammalian cells

Depositor

Alice Ting

Publication

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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plenti-px330-CRBN-T1-pGK-Pur

Plasmid

#107382

Purpose

Mammalian expression CRISPR/Cas9

Depositor

William Kaelin

Publication

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

sgRNA targeting CRBN (CRBN Human)

Use

Lentiviral; Crispr

Expression

Mammalian

Promoter

U6

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHACK-GAL4>QF2(newHA1)

Plasmid

#104873

Purpose

HACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of inserts

Depositor

Christopher Potter

Publication

Lin et al Genetics. 2016 Jun 22. pii: genetics.116

Insert

T2A-QF2

Use

CRISPR

Expression

Insect

Available since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AAV-syn-ChR2-Kv-P2A-H2b-mRuby2

Plasmid

#89570

Purpose

Channelrhodopsin localized to neuronal soma with simultaneous nuclear labeling

Depositor

McLean Bolton

Publication

Baker et al Elife. 2016 Aug 15;5. pii: e14193. doi:

Inserts

Channelrhodopsin
Histone 2B

Use

AAV

Tags

mRuby2

Expression

Mammalian

Mutation

H134R

Promoter

synapsin

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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EGFP-BAF_L58R

Plasmid

#101776

Purpose

Expresses EGFP tagged mutant BAF (L58R) in human cells

Depositor

Daniel Gerlich

Publication

Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

Insert

BAF (BANF1) (BANF1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

L58R mutation

Promoter

EF1a

Available since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCDNA3-HA-Akt1-aa1-149

Plasmid

#73410

Purpose

Expresses HA tagged aa1-149 of AKT1

Depositor

Jie Chen

Publication

Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

Insert

AKT1 (AKT1 Human)

Tags

HA tag

Expression

Mammalian

Mutation

Expresses aa 1-149 only of AKT1

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
p2B-55

Plasmid

#177924

Purpose

Expresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter

Depositor

Vadim Gladyshev

Publication

Barre et al (unpublished)

Insert

Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter

Expression

Bacterial and Yeast

Mutation

V9I; F67S; G72P, F86Y; R171K

Promoter

TDH3

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GLuc-FLAG-CB1

Plasmid

#158066

Purpose

Membrane expression detection of Cannabinoid receptor 1

Depositor

Adolfo Rivero-Muller

Publication

Rodriguez-Rodriguez et al Sci Rep. 2020 Oct 23;10(1):18191. doi: 1

Insert

CB1 (CNR1 Human)

Use

AAV

Tags

Gaussia Luciferase

Expression

Mammalian

Mutation

contains 4Kb of 3' UTR

Promoter

pCAG

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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SauCas9 PE2*

Plasmid

#169851

Purpose

Mammalian Expression, sauCas9 based prime editor

Depositor

Scot Wolfe

Publication

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SaCas9-N580A-M-MLV

Tags

bpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…

Expression

Mammalian

Promoter

cmv

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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Kv7.5/pcDNA3.1

Plasmid

#111498

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Publication

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

potassium voltage-gated channel subfamily KQT member 5 isoform 1 [Homo sapiens] (KCNQ5 Human)

Expression

Mammalian

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-IRES-hrGFP-mWnt3a

Plasmid

#89771

Purpose

Expresses mouse Wnt3a in mammalian cells

Depositor

Ken-Ichi Takemaru

Publication

Rodriguez et al J Neurosci. 2014 Jul 30;34(31):10285-97.

Insert

mouse Wnt3a (Wnt3a Mouse)

Use

AAV

Tags

None

Promoter

CMV

Available since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pLJM60-FLAG-SLC38A9.1 (1-119aa)

Plasmid

#71862

Purpose

lentiviral stable expression

Depositor

David Sabatini

Publication

Wang et al Science. 2015 Jan 9;347(6218):188-94. do

Insert

SLC38A9.1 (SLC38A9 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized, first 119 amino acids

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
MSCV-NSD3-short

Plasmid

#72552

Purpose

expresses 3*FLAG tagged human NSD3-short

Depositor

Christopher Vakoc

Publication

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

NSD3-short (NSD3 Human)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

none

Promoter

LTR

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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