We narrowed to 12,917 results for: Emb;
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Plasmid#68207PurposeProvides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid partDepositorInsertCas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removed; human codon optimis…Available SinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGFP AH2 tensin
Plasmid#50514PurposeThis plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.DepositorInsertTensin (TNS1 Chicken)
TagsGFPExpressionMammalianMutationAA659--761, including actin homology 2 domain of …PromoterCMVAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SSA TLR (Ani)
Plasmid#45577PurposeCodes for the SSA-TLR with the target site for I-Ani I in a lentiviral backboneDepositorInserts5' iRFP arm
eGFP with I-Ani I TS
+3 mCherry
3' iRFP arm
UseLentiviralExpressionBacterial and MammalianMutationembedded I-Ani I TS from 163-185bp, truncated 25 …PromoterSFFVAvailable SinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Btsz2
Plasmid#40068DepositorAvailable SinceOct. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBAD33(A-H*HA-N)
Plasmid#187086PurposeMedium expression of Complex I with subunits N and H -HA taggedDepositorInsertnuoA-N
TagsHA tags nuoN, nuoHExpressionBacterialAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pME-V5-mClover-KRAS4A-G12D (JDW 813)
Plasmid#242567PurposeGateway middle entry clone containing an mClover3 fused human KRAS4A G12D.DepositorInsertKRAS4A-G12D (KRAS Human)
UseGateway CloningAvailable SinceSept. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA
Plasmid#228530PurposeFirefly luciferase in fusion with a fragment of human Skp1 protein through a flexible linker and a TEV viral protease cleavage site. Expression of fLuc protein is deinhibited by TEV site cleavage.DepositorInsertmyc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA (SKP1 Synthetic, Photinus pyralis, Human)
TagsHA and mycExpressionMammalianPromoterCMVAvailable SinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM11-SUMO3- sfGFP-KSR1-CA3 (N-KSR)
Plasmid#217767PurposeFluorescent reporter for glucosyl-ceramide (putative, mammalian expression)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
UseProtein purification (6xhis tag, tev site, sumo3 …TagssfGFPExpressionBacterialMutationCA3 domain aa 317-400PromoterT7 promoterAvailable SinceJune 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPW3761 : CMVd3-HsIRE1(WLLI-GSGS)[359-362]-HaloTag
Plasmid#185683PurposeLow-level transient expression of IF2 mutant HsIRE1 with a C-terminal HaloTag.DepositorAvailable SinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVC-DNAJB6b
Plasmid#175065Purposeexpresses mVenus and mCherry tagged DNAJB6b in mammalian cellsDepositorInsertDNAJB6b (DNAJB6 Human)
TagsV5 and mVenus + mCherryExpressionMammalianMutationmVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…PromoterCMV PromoterAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyso-INPP4B-mCherry
Plasmid#184055PurposeLysosome targeted inositol polyphosphate-4-phosphatase type II B; tagged with mCherry.DepositorTagsLysosome-associated membrane protein 1 (LAMP1). a…ExpressionMammalianPromoterCMVAvailable SinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
6xHis(TEV)-dDrp1-StrepII
Plasmid#174421PurposeExpresses D. melanogaster Drp1 in bacteriaDepositorAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-delta-TMD-VAMP1-IRES-Hygromycin
Plasmid#134866PurposeExpresses EGFP-tagged mutant of VAMP1DepositorInsertVesicle-associated membrane protein 1 (VAMP1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdeleted amino acids 97-118PromoterEF1aAvailable SinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-P13-ss
Plasmid#137065PurposeE. coli expression clone (T7lac promoter) for mature P13 (aa 22-179) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAC66426.1 (BB_0034 Borrelia burgdorferi B31)
TagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationmature P13: lacks the P13 signal peptidePromoterT7lacAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
human rSHIP2 (18-893)
Plasmid#128568PurposeExpression of human SHIP2 in bacteriaDepositorInsertSHIP2 (INPPL1 Human)
TagsHis Xpress (N-terminal)ExpressionBacterialMutationSHIP2 coding sequence from amino acid 18 (A) till…Available SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKIF1C-2xFLAG
Plasmid#130979PurposeExpression of KIF1C-2xFLAG in mammalian cells.DepositorInsertKIF1C-2xFLAG (KIF1C Human)
Tags2xFLAGExpressionMammalianMutation4 silent mutations that make this construct resis…PromoterCMVAvailable SinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
SERK3/BAK1 BAKNcFus_pECIA14
Plasmid#114775PurposePrey vector SERK3/BAK1 BAKNcFus_pECIA14 should be used with bait vector SERK3/BAK1 BAKNcFus_pECIA2.DepositorAvailable SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4 h-Stx3 resistant to shRNA #304-2xMyc/His
Plasmid#99746PurposeExpresses human Stx3 with a C-term myc-myc-his that is resistant to shRNA #304 from Sigma Aldrich.DepositorInsertStx3 (STX3 Human)
Tagsmyc-myc-hisExpressionMammalianMutationMade six (6) silent mutations to confer resistanc…PromoterCMV/TOAvailable SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-FLAG-GFP-Ubl4A-H1(90-107)
Plasmid#87202PurposeTo express Ubl4A (90-107 residues) tagged with FLAG-EGFP at N- terminus.DepositorInsertUBL4A (UBL4A Human)
TagsFLAG and GFPExpressionMammalianMutationResidues from 90-107 are inserted.PromoterCMVAvailable SinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-FLAG-GFP-Ubl4A-C(90-157)
Plasmid#86923PurposeTo express Ubl4A (90-157 residues) tagged with FLAG-EGFP at N- terminus.DepositorInsertUBL4A (UBL4A Human)
TagsFLAG and GFPExpressionMammalianMutationResidues from 1-89 are deletedPromoterCMVAvailable SinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only