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pSB96 - pL2_pSB90_2x35S::fLUC-I::tNOS

Plasmid

#123188

Purpose

binary plant vector for transient fLUC (with intron) expression

Depositor

Erin Cram, Carolyn Lee-Parsons

Publication

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::fLUC-I::tNOS and VirGN54D in the vector backbone

Use

Luciferase

Expression

Plant

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC1076 - prRosa26v1 LSL FLAG-SpCas9n NeoR

Plasmid

#113162

Purpose

A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9n and a NeoR selectable marker

Depositor

Brandon Harvey

Publication

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

SpCas9

Tags

FLAG

Expression

Mammalian

Mutation

D10A

Promoter

CAG

Available since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pNIC-ZB-SPRTN-E112A-FL

Plasmid

#110218

Purpose

Expression in E. coli of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, with His and ZB tags at N-terminus.

Depositor

Kristijan Ramadan

Publication

Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

Insert

SPRTN (SPRTN Human)

Tags

His6 and ZB

Expression

Bacterial

Mutation

E112A, P296L (see depositor comments below)

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pSpCas9_BB_2A-GFP_MAPRE3-gRNA#1

Plasmid

#107729

Purpose

Knock out of EB3 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Publication

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)

Expression

Mammalian

Promoter

U6

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGEX2TK-YAP-WW1-RRK

Plasmid

#104306

Purpose

bacterial expression of RKK mutant WW domain 1 of YAP; RRK mutant binds to PPxY where Y is phosphorylated

Depositor

Marius Sudol

Insert

YAP WW1 domain (YAP1 Human)

Tags

GST

Expression

Bacterial

Mutation

WW domain 1 RRK mutant (TTAAATCACATCGATCAG to AGA…

Promoter

tac

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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Y587E EphA2 pcDNA3

Plasmid

#102730

Purpose

Expression of EphA2 in mammalian cells

Depositor

Jin Chen

Publication

Fang et al J Biol Chem. 2008 Jun 6;283(23):16017-26

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EphA2 (Epha2 Mouse)

Expression

Mammalian

Mutation

Y587E (TAT > GAG)

Promoter

CMV

Available since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pET151-Vps4(101-437)

Plasmid

#87735

Purpose

Bacterial expression of N-terminally truncated S. cerevisiae Vps4

Depositor

Chris Hill

Publication

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)

Tags

6xHis-V5-TEV

Expression

Bacterial

Mutation

deleted amino acids 1-100

Promoter

T7

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA4/TO/FLAG-hPMR1-Tev-Bio

Plasmid

#80125

Purpose

Expresses human PMR1 endonuclease with N-terminal FLAG and C-terminal biotinylation tags

Depositor

Daniel Schoenberg

Publication

Gu et al RNA. 2012 Jun;18(6):1186-96. doi: 10.126

Insert

PXDNL-003 (PXDNL Human)

Tags

FLAG and biotinylation

Expression

Mammalian

Promoter

CMV promoter

Available since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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SIK1 gRNA (BRDN0001146021)

Plasmid

#77894

Purpose

3rd generation lentiviral gRNA plasmid targeting human SIK1

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SIK1 (SIK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PIP5K1C gRNA (BRDN0001148198)

Plasmid

#77665

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIP5K1C

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIP5K1C (PIP5K1C Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PIP5K1C gRNA (BRDN0001145736)

Plasmid

#77666

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIP5K1C

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIP5K1C (PIP5K1C Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP2K2 gRNA (BRDN0001147805)

Plasmid

#77600

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K2

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K2 (MAP2K2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP2K2 gRNA (BRDN0001147718)

Plasmid

#77601

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K2

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K2 (MAP2K2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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LRRK2 gRNA (BRDN0001146754)

Plasmid

#77330

Purpose

3rd generation lentiviral gRNA plasmid targeting human LRRK2

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

LRRK2 (LRRK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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DCLK1 gRNA (BRDN0001147487)

Plasmid

#76747

Purpose

3rd generation lentiviral gRNA plasmid targeting human DCLK1

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DCLK1 (DCLK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKD3 gRNA (BRDN0001146046)

Plasmid

#75952

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKD3

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKD3 (PRKD3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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IRAK4 gRNA (BRDN0001149331)

Plasmid

#75666

Purpose

3rd generation lentiviral gRNA plasmid targeting human IRAK4

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

IRAK4 (IRAK4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SGK3 gRNA (BRDN0001147184)

Plasmid

#75542

Purpose

3rd generation lentiviral gRNA plasmid targeting human SGK3

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

C8orf44-SGK3,SGK3 (SGK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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RPS6KA1 gRNA (BRDN0001148103)

Plasmid

#75498

Purpose

3rd generation lentiviral gRNA plasmid targeting human RPS6KA1

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RPS6KA1 (RPS6KA1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET23a-MmRPS27a

Plasmid

#69559

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Publication

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

RPS27A (RPS27A Human, Mouse)

Expression

Bacterial

Mutation

Changed Alanine 133 (human RPS27a) to Glycine (mo…

Promoter

T7

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only