We narrowed to 14,648 results for: NTS;

1. Ash1-5p

   Plasmid

   #169712

   Purpose

   Bacterial Expression of truncated yeast Ash1 including encoding amino acids 420-480 with phosphorylation sites 429 and 470 removed

   Depositor

   Tanja Mittag

   Article

   Martin et al J Am Chem Soc. 2016 Nov 30;138(47):15323

   Insert

   Ash1 (ASH1 Budding Yeast)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Truncation including encoding amino acids 420-480…

   Promoter

   T7

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGMC00008

   Plasmid

   #166724

   Purpose

   sgRNA cloning backbone

   Depositor

   Raj Chari

   Article

   Choi et al Nat Commun. 2020 Jun 1;11(1):2718. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pgRNA_CV2

   Plasmid

   #167165

   Purpose

   pgRNA_CV2 is derived from gRNA_cloning vector (Addgene plasmid ID: 41824) by adding about 80 bps from the sgRNA sequence as well as an AgeI site. In the literature, sgRNA_AL is used as an alias.

   Depositor

   Alexander Loewer

   Article

   Sheng et al Cell Rep. 2019 Apr 2;27(1):48-58.e7. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 promoter

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUPD2 AtU6-26:AsCas12a_DR (GB1442)

   Plasmid

   #160574

   Purpose

   AsCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   AtU6-26:AsCas12a_DR
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUPD2 LbCas12a (GB1439)

   Plasmid

   #160573

   Purpose

   Human codon optimized coding sequence of Cas12a from Lachnospiraceae CRISPR system (LbCas12a).

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   LbCas12a
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUPD2 AsCas12a (GB1438)

   Plasmid

   #160572

   Purpose

   Human codon optimized coding sequence of Cas12a from Acidominococcus sp (AsCas12a).

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   AsCas12a
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDONR221 NLRP1 A59P

   Plasmid

   #166816

   Purpose

   Gateway pDONR vector encoding NLRP1 with the patient mutation A59P (Herlin et al., 2020) (no stop codon)

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Mutation

   A59P

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONR221 Ub-NLRP1 L1215

   Plasmid

   #166828

   Purpose

   pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate a L1215 N-terminus (no stop)

   Depositor

   Daniel Bachovchin

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Ubiquitin

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDONR221 Ub-NLRP1 S1213

   Plasmid

   #166827

   Purpose

   pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate the native S1213 N-terminus (no stop)

   Depositor

   Daniel Bachovchin

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Ubiquitin

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR221 Ub-NLRP1 L1218

    Plasmid

    #166829

    Purpose

    pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate a L1218 N-terminus (no stop)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Ubiquitin

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDONR221 NLRP1 FIIND (F1079M-L1373)

    Plasmid

    #166831

    Purpose

    Gateway pDONR vector containing NLRP1 FIIND (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Available Since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. dTAG NLRP1 P1214R

    Plasmid

    #166826

    Purpose

    dTAG (degron tag) NLRP1 for ligand-controlled N-terminal degradation

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Expression

    Mammalian

    Mutation

    P1214R

    Promoter

    hPGK

    Available Since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAT-9251

    Plasmid

    #124226

    Purpose

    Bacterial SpCas9-HF1 expression

    Depositor

    Ervin Welker

    Article

    Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

    Insert

    SpCas9-HF1
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    TetR/TetA

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mTurquoise2-RnBarr1

    Plasmid

    #137795

    Purpose

    Visualization of beta-arrestin2

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Rat beta-arrestin 1 (Arrb1 Rat)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. RnAT1aR DRY->AAY-p2A-mCherry

    Plasmid

    #137784

    Purpose

    Visualization of the At1aR DRY mutant

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Rat At1a receptor; DRY mutant (Agtr1a Rat)
    

    Expression

    Mammalian

    Mutation

    DRY -> AAY

    Promoter

    CMV

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCEP4-myc-H2-Dd-VN

    Plasmid

    #135517

    Purpose

    Mammalian expression of VN-fused and myc-tagged H2-Dd

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    H2-Dd (H2-D1 Mouse)
    

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Mutation

    C121S

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCEP4-myc-H2-Ld-VN

    Plasmid

    #135520

    Purpose

    Mammalian expression of VN-fused and myc-tagged H2-Ld

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    H2-Ld
    

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28P_dCrk

    Plasmid

    #131145

    Purpose

    Expresses 6XHis::dCrk (based on crk isoform RA).

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    Crk (Crk Fly)
    

    Tags

    6XHis

    Expression

    Bacterial

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFA0057

    Plasmid

    #131784

    Purpose

    Guide RNA (gGFP) and Cas9 expression plasmid for cleaving pFA6 series GFP C-terminal tagging cassettes, including GFP-His3MX6. Used to perform CRISPR-Swap of alleles.

    Depositor

    Frank Albert

    Article

    Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.

    Insert

    20mer GFP cassette guide (gGFP) and 5' sgRNA
    

    Expression

    Bacterial and Yeast

    Available Since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKD284

    Plasmid

    #125073

    Purpose

    Expression of SO_4488 under Ptac

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    SO_4488 (SO_4488 )
    lacI (lacI S286L- Please see depositor comments below)
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge