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pcDNA5/FRT/TO APEX2-GFP

Plasmid

#129640

Purpose

Express APEX2-GFP fusion in mammalian cells

Depositor

Nicholas Ingolia

Article

Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

Insert

APEX2-GFP

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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GST-RBD

Plasmid

#15247

Depositor

Martin Schwartz

Article

Ren et al EMBO J. 1999 Feb 1. 18(3):578-85.

Insert

Rhotekin (Rtkn Mouse)

Tags

GST

Expression

Bacterial

Mutation

amino acids 7-89 (Rho binding domain)

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-TRPT1

Plasmid

#184244

Purpose

For TRPT1 expression in mammalian cells

Depositor

Karla Feijs

Article

Weixler et al Nucleic Acids Res. 2022 Sep 9;50(16):942

Insert

TRPT1 (TRPT1 Human)

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Hook1-EGFP

Plasmid

#171941

Purpose

Expresses Hook1 in mammalian cells

Depositor

Cheng-han Yu

Article

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

Hook1

Tags

EGFP

Expression

Mammalian

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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poRBS

Plasmid

#154125

Purpose

Expresses orthogonal rRNAs operon in bacterial cells

Depositor

Alexander Mankin

Article

Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:

Insert

engineered bacterial rRNAs operon under control of pL promotor

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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zCREST3:redCas9; control_gRNA

Plasmid

#199334

Purpose

pDEL157; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous control sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

zCREST3
Cas9
control sgRNA

Use

Tol2 destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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Cre Stoplight 2.4

Plasmid

#37402

Purpose

Cre activity reporter plasmid; Zsgreen is expressed in cells without Cre, mCherry is expressed with Cre

Depositor

Thomas Hughes

Article

Yang et al Biotechniques. 2001 Nov;31(5):1036, 1038

Insert

Cre Stoplight 2.4

Tags

ZsGreen1 and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pRBS wt

Plasmid

#154126

Purpose

Expresses wt rRNAs operon in bacterial cells

Depositor

Alexander Mankin

Article

Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:

Insert

wt bacterial rRNAs operon under control of pL promotor

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pSNAP-macroH2A1.2 Zeo

Plasmid

#132428

Purpose

Expressing SNAP-tagged histone macroH2A1.2 in mammalian cells

Depositor

John M. Greally

Article

Sato et al J Cell Biol. 2019 Jun 3;218(6):1810-1823

Insert

macroH2A1.2

Expression

Mammalian

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_crGE

Plasmid

#172933

Purpose

Crowding FRET sensor with mCerulean3 and mCitrine for mammalian cell expression. Linker comprises 2 helices and 3 GSG domains.

Depositor

Arnold Boersma

Article

Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

Insert

CrowdingSensorGE

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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HBx

Plasmid

#65463

Purpose

expresses HBx in mammalian cells

Depositor

Wang-Shick Ryu

Article

Cha et al Hepatology. 2004 Jun;39(6):1683-93.

Insert

HBV X protein

Tags

Flag

Expression

Mammalian

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pLVX-EGFP-HOTag3

Plasmid

#225677

Purpose

Used to generate stable cell lines that express EGFP-HOTag3. This plasmid serves as a control for pLVX-PERK(LD)-EGFP-HOTag3.

Depositor

Likun Wang

Article

Xu et al Cell Rep. 2024 Jun 25;43(6):114358. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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AbVec2.1-IGLC2-MscI

Plasmid

#80797

Purpose

Expression of immunoglobulin light chains in mammalian cells, human lambda 2 constant region

Depositor

Hedda Wardemann

Article

Tiller et al J Immunol Methods. 2009 Oct 31;350(1-2):

Type

Empty backbone

Expression

Mammalian

Promoter

hCMV IE1 gene promoter

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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mScarlet-Rab9a

Plasmid

#169074

Purpose

Express human Rab9a in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rab9a (RAB9A Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CMV-eBRIC

Plasmid

#241800

Purpose

Bioluminescence indicator for Ca2+ imaging in mammalian cells

Depositor

Huiwang Ai

Article

Tian et al ACS Sens. 2025 Jul 29. doi: 10.1021/acss

Insert

eBRIC

Expression

Bacterial

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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p-miniTurbo-HA

Plasmid

#209637

Purpose

Expression miniTurbo-HA in mammalian cells

Depositor

Xiang Zhou

Article

Lu et al Nucleic Acids Res. 2024 Mar 7:gkae126. d

Insert

miniTurbo

Expression

Mammalian

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-STONIN2

Plasmid

#177885

Purpose

Espresses Stonin2 in mammalian cells

Depositor

Juan Bonifacino

Article

Martina et al J Cell Biol. 2001 May 28;153(5):1111-20.

Insert

stonin2 (STON2 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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LAMP1-GFP

Plasmid

#16290

Depositor

Ron Vale

Article

Minin et al J Cell Sci. 2006 Feb 15. 119(Pt 4):659-7

Insert

LAMP1/RE72002

Tags

EGFP

Expression

Insect

Promoter

Ac5

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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His6_BcLOV4_Q355N_mCherry_BamUK

Plasmid

#119762

Purpose

Encodes mCherry-tagged BcLOV4 permanently-"lit" mutant with Q355N mutation for expression in bacterial cell

Depositor

Brian Chow

Article

Glantz et al Methods Enzymol. 2019;622:249-270. doi:

Insert

BcLOV4-Q355N_mCherry

Tags

mCherry

Expression

Bacterial

Mutation

Mutated glutamine at position 355 to asparagine

Promoter

T7

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pQE31-rsEGFP2

Plasmid

#102879

Purpose

Expresses rsEGFP2 in bacterial cells

Depositor

Stefan Jakobs

Article

Grotjohann et al eLife. 2012 Dec 31:1:e00248. doi: 10.755

Insert

rsEGFP2

Tags

6xHisTag

Expression

Bacterial

Available Since

Jan. 12, 2018

Availability

Academic Institutions and Nonprofits only

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