We narrowed to 4,533 results for: Gaa

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pk335-CARGO-6mer-36kb-USF

Plasmid

#227461

Purpose

6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 36kb Upstream Fgf5

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-CARGO-5mer-36kb-USF

Plasmid

#227462

Purpose

5-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 36kb Upstream Fgf5

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-CARGO-6mer-26kb-USP

Plasmid

#227444

Purpose

6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 26kb Upstream Prdm8

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-CARGO-6mer-7.5kb-USP

Plasmid

#227448

Purpose

6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 7.5kb Upstream Prdm8

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-KAE1

Plasmid

#232891

Purpose

Plasmid expressing Cas9 and gRNA GATGACAACTGAATGCAGAG which targets the KAE1 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

KAE1 gRNA (KAE1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-SAM50

Plasmid

#232895

Purpose

Plasmid expressing Cas9 and gRNA AATAGTTTATGTAAGAACAG which targets the SAM50 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

SAM50 gRNA (SAM50 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pML104-HygMx4-URA3-2

Plasmid

#232885

Purpose

Plasmid expressing Cas9 and gRNA TCGTACCACCAAGGAATTAC which targets the URA3 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

URA3 gRNA (URA3 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pGM63

Plasmid

#220169

Purpose

CRISRPi guide RNA against TOMM70A

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TOMM70A (sgRNA) (TOMM70 Human)

Use

Lentiviral

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-YFR054C

Plasmid

#232900

Purpose

Plasmid expressing Cas9 and gRNA GCTCAAAGAAACAATTAGAG which targets the YFR054C gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

YFR054C gRNA (YFR054C Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-SRP14

Plasmid

#232896

Purpose

Plasmid expressing Cas9 and gRNA GAAAACAAAATGCTCAACAG which targets the SRP14 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

SRP14 gRNA (SRP14 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-VHT1

Plasmid

#232898

Purpose

Plasmid expressing Cas9 and gRNA TGCGGCCACACTGAAATACG which targets the VHT1 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

VHT1 gRNA (VHT1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-ADE13

Plasmid

#232887

Purpose

Plasmid expressing Cas9 and gRNA GTAGCAGCAAAAGAAGACAA which targets the ADE13 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

ADE13 gRNA (ADE13 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-LAS17

Plasmid

#232892

Purpose

Plasmid expressing Cas9 and gRNA AATACCCTGAATTCTGCCGG which targets the LAS17 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

LAS17 gRNA (LAS17 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML104-LEU2-2

Plasmid

#232883

Purpose

Plasmid expressing Cas9 and gRNA GGTAGTGTTAGACCTGAACA which targets the LEU2 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

LEU2 gRNA (LEU2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pEND-225_Cacnes_mKO2

Plasmid

#225612

Purpose

Replicative vector for orange fluorescent reporter protein mKO2 strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mKO2

Depositor

Marc Güell

Article

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Insert

mKO2

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Codon optimized for Cutibacterium acnes

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAG155

Plasmid

#226744

Purpose

CRISPRi guide RNA against TOMM20

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TOMM20 (TOMM20 Human)

Use

Lentiviral

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG-sgRNA.PRDM1-ZEB2_CRISPRd

Plasmid

#216171

Purpose

Express the gRNA targeting the PRDM1-binding site in the human ZEB2 locus for the CRISPRd experiment

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Insert

sgRNA- PRDM1.bs- ZEB2.locus (PRDM1 Human)

Use

Lentiviral

Tags

EGFP

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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UCK2_Deletion_gRNA_1

Plasmid

#204672

Purpose

Dual gRNA plasmid for UCK2 deletion

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

uridine-cytidine kinase 2 (UCK2 Human)

Use

Lentiviral

Promoter

U6

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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U6-sgRNA-hcr5

Plasmid

#193661

Purpose

Expression of tandem pre-sgRNA array hcr5 for LbCas12a

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

U6-DNMT3B-sgRNA-KLF4-sgRNA-TET1-sgRNA-PRR5L-sgRNA-CFTR-sgRNA-tRNA

Expression

Mammalian

Promoter

U6

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-mCherry-SPASTIN, L177D

Plasmid

#180649

Purpose

Lentiviral expression vector for SPASTIN. Has L177D mutation. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. SPASTIN starts on M87. Internal ID: WISP20-45.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Starts at M87, L177D mutation, has siRNA resistan…

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-NDUFB5_sgRNA1

Plasmid

#201606

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

NDUFB5 (NDUFB5 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/shCol1a1/Scarlet-Seq1.1

Plasmid

#201401

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1

Expression

Mammalian

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/Col1a1/GFP4_Seq 1.1

Plasmid

#201400

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1

Expression

Mammalian

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgGPAA1 guide 1

Plasmid

#193608

Purpose

GPAA1 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgGPAA1 guide 1 (GPAA1 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shTgm5-1

Plasmid

#180394

Purpose

Producing AAV that encodes mouse Tgm5 shRNA-1 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shTgm5-1 (Tgm5 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_BLK_E2-1

Plasmid

#124474

Purpose

For CRISPR-mediated epigenome editing of human BLK gene locus

Depositor

Iouri Chepelev

Article

Saint Just Ribeiro et al Front Genet. 2022 Sep 7;13:1008582. doi:

Insert

BLK_E2-1_gRNA (BLK Human)

Expression

Mammalian

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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L2_2xNOP1gRNA-Cas9-CsA

Plasmid

#136140

Purpose

Plasmid with 2 different NOP1-gRNA. Targets 500 bp apart in the genome. To tranform Mp as CRISPR control. Contains Cas9 and HygR

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-35S:HygR p5-MpU6:NOP1gRNA2 p5-MpU6:NOP1gRNA1 p5-MpEF1a:Cas9-NLS

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1P PRIM2_2

Plasmid

#160796

Purpose

Suppress PRIM2

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPRIM2_2

Use

Lentiviral

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P PRIM2_1

Plasmid

#160795

Purpose

Suppress PRIM2

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPRIM2_1

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P EXO5_1

Plasmid

#160783

Purpose

Suppress EXO5

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shEXO5_1

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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