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Showing: 1141 - 1170 of 2836 results

  6. pCAGGS-SMS1-TEV-Twin-Strep

    Plasmid
    #202528
    Purpose
    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cell
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Insert
    SGMS1 (SGMS1 Human)
    Use
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    deleted stop codon (TAA)
    Promoter
    CAG and chicken β-actin promoter
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202528-plasmid-map-sequence-id-406371

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  7. pAAV2-CAG-3xNLS-AausFP1

    Plasmid
    #191096
    Purpose
    To express a bright green FP to label eucaryotic cell nuclei. To be used in tissue-clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    3xNLS-AausFP1
    Use
    AAV and Synthetic Biology
    Tags
    Three repeats of the nuclear localizzation seque…
    Expression
    Mammalian
    Mutation
    Optimized to Human codon usage
    Promoter
    CMV enhancer and CAG
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191096-plasmid-map-sequence-id-380123

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  11. pAAV-hSyn-mCherry.3xFLAG.NOS1AP-S-WPRE

    Plasmid
    #127867
    Purpose
    pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP-S fusion protein under the hSyn promoter
    Depositor
    Florian Freudenberg
    Article
    Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)
    Insert
    Nos1ap (Nos1ap Mouse, Human)
    Use
    AAV
    Tags
    3xFLAG and mCherry
    Expression
    Mutation
    constructed by blunt end fusion of the 5’ 51 nucl…
    Promoter
    hSyn
    Available since
    Aug. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    127867-plasmid-map-sequence-id-252384

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  12. pAAV2-CAG-mScarlet-I-Cdt1

    Plasmid
    #191100
    Purpose
    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    mScarlet-I-Cdt1 (30-120)
    Use
    AAV and Synthetic Biology
    Tags
    mScarlet-I fused to residues 30-120 of human Cdt1…
    Expression
    Mammalian
    Mutation
    Optimized to human codon usage
    Promoter
    CMV enhancer and CAG
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191100-plasmid-map-sequence-id-380122

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  15. pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-500-WPRE

    Plasmid
    #174134
    Purpose
    pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-500 fusion protein under the hSyn promoter
    Depositor
    Florian Freudenberg
    Article
    Freudenberg et al EBioMedicine. 2021 Aug 26;71:103565. doi
    Insert
    Nos1ap (Nos1ap Mouse)
    Use
    AAV
    Tags
    3xFLAG and mCherry
    Expression
    Mutation
    Only contains the sequences coding for amino acid…
    Promoter
    hSyn
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174134-plasmid-map-sequence-id-345686

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  21. pJET-osr1-KI-donor

    Plasmid
    #184875
    Purpose
    Donor template for knock-in of p2a-CreERT2 into the medaka osr1 locus via CRISPR/Cas9-mediated homology directed repair
    Depositor
    Christoph Winkler
    Article
    Hui et al Development. 2022 May 20. pii: 275483. d
    Inserts
    Odd-Skipped Related Transcription Factor 1 5' homology arm
    Odd-Skipped Related Transcription Factor 1 3' homology arm
    p2a-CreERT2
    Myosin light chain 2 promoter
    EGFP
    Use
    CRISPR and Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    184875-plasmid-map-sequence-id-366330

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  22. pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-503-WPRE

    Plasmid
    #174133
    Purpose
    pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-503 fusion protein under the hSyn promoter
    Depositor
    Florian Freudenberg
    Article
    Freudenberg et al EBioMedicine. 2021 Aug 26;71:103565. doi
    Insert
    Nos1ap (Nos1ap Mouse)
    Use
    AAV
    Tags
    3xFLAG and mCherry
    Expression
    Mutation
    Only contains the sequences coding for amino acid…
    Promoter
    hSyn
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174133-plasmid-map-sequence-id-345689

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  23. pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-427-WPRE

    Plasmid
    #174135
    Purpose
    pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-427 fusion protein under the hSyn promoter
    Depositor
    Florian Freudenberg
    Article
    Freudenberg et al EBioMedicine. 2021 Aug 26;71:103565. doi
    Insert
    Nos1ap (Nos1ap Mouse)
    Use
    AAV
    Tags
    3xFLAG and mCherry
    Expression
    Mutation
    Only contains the sequences coding for amino acid…
    Promoter
    hSyn
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174135-plasmid-map-sequence-id-345687

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Showing: 1141 - 1170 of 2836 results