Addgene

We narrowed to 32,562 results for: IND

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pcDNA3.1 3xFlag IFIT5

Plasmid

#53556

Purpose

mammalian expression of IFIT5

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT5 (IFIT5 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
pET28a(+) 6xHis-3xFlag-IFIT5

Plasmid

#53561

Purpose

bacterial expression of IFIT5

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT5 (IFIT5 Human)

Use

Tags

3xFLAG, 6xHIS, and TEV site

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
ires-eGFP(+HAs) donor-1

Plasmid

#87865

Purpose

Express eGFP reporter for HDR-induced knock-in

Depositor

Bo Feng

Article

He et al Nucleic Acids Res. 2016 May 19;44(9):e85

Insert

eGFP

Use

Homologous recombination for gapdh loci

Tags

Expression

Mutation

Promoter

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pKV015

Plasmid

#64766

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, HA-GFP to a T7-RFP tag, invariable V5 tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Verzijlbergen et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

Insert

RITE cassette

Use

Rite cassette

Tags

Expression

Mutation

Promoter

none

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNAIIIB cdc42 WT

Plasmid

#61635

Purpose

mammalian expression of cdc42 wt

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

cdc42 (CDC42 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pCDNAIIIB rhoA WT

Plasmid

#61631

Purpose

mammalian expression of rhoA

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

rhoA (RHOA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pJTX-GL3

Plasmid

#11981

Purpose

Reporter gene with mouse c-jun promoter and point mutation of AP1 binding site

Depositor

Ron Prywes

Article

Clarke et al Mol Cell Biol. 1998 Feb . 18(2):1065-73.

Insert

c-jun promoter (Jun Mouse)

Use

Luciferase

Tags

Luc

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pJSTX-GL3

Plasmid

#11982

Purpose

Reporter gene with mouse c-jun promoter and point mutation of MEF2 and AP1 binding sites

Depositor

Ron Prywes

Article

Clarke et al Mol Cell Biol. 1998 Feb . 18(2):1065-73.

Insert

c-jun promoter (Jun Mouse)

Use

Luciferase

Tags

Luc

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pJSX-GL3

Plasmid

#11980

Purpose

Reporter gene with mouse c-jun promoter and point mutation of MEF2 binding site

Depositor

Ron Prywes

Article

Clarke et al Mol Cell Biol. 1998 Feb . 18(2):1065-73.

Insert

c-jun promoter (Jun Mouse)

Use

Luciferase

Tags

Luc

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pKan-PCUP1-AID*(N)

Plasmid

#99525

Purpose

N-terminal AID* degron cassette with CUP1 promoter and KanR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-117) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

Expression

Mutation

Promoter

CUP1

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a(+) 6xHis-TEV-IFIT5

Plasmid

#53560

Purpose

bacterial expression of IFIT5

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT5 (IFIT5 Human)

Use

Tags

6xHIS and TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
pHyg-AID(1-114)-8myc

Plasmid

#99516

Purpose

Truncated C-terminal AID-8myc degron cassette with HygR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(1-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

8xMyc

Expression

Mutation

Promoter

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pHyg-AID(31-114)-9myc

Plasmid

#99517

Purpose

Truncated C-terminal AID-9myc degron cassette with HygR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(31-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Expression

Mutation

Promoter

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pHCKan-yibDp-CBD-hGLY

Plasmid

#134596

Purpose

Low Phosphate inducible hGLYAT2 with N-terminal chitin binding domain tag

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

human glycine acyltransferase 2 (GLYATL2 )

Use

Tags

chitin binding domain

Expression

Bacterial

Mutation

Promoter

yibD

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA10_no stop

Plasmid

#83848

Purpose

Gateway donor vector for IAA10 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

indoleacetic acid-induced protein 10 (IAA10 Mustard Weed)

Use

Gateway donor vector

Tags

Expression

Mutation

no stop codon

Promoter

Available since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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p5xUAS-Flag-NPM1wt

Plasmid

#131822

Purpose

4-hydroxytamoxifen inducible vector expressing Flag-NPM1wildtype (human) in mammalian cells through transduction

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

NPM1 (NPM1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Delta(Pro-MP)ADAM17-HA

Plasmid

#65221

Purpose

Expression of HA tagged ADAM17 lacking prodomain and metalloprotease domain in mammalian cells

Depositor

Axel Ullrich

Article

Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

Insert

Delta(Pro-MP)ADAM17 (ADAM17 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

deleted AA 18-473

Promoter

CMV

Available since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pRetroX GFP T2A Cre

Plasmid

#63704

Purpose

Inducible expression of GFP T2A Cre fusion in mammalian cells

Depositor

Floris Foijer

Article

Foijer et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

Insert

T2A Cre

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

tight TRE promoter

Available since

April 22, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xFlag IFIT3

Plasmid

#53553

Purpose

mammalian expression of IFIT3

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT3 (IFIT3 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
pHyg-AID*

Plasmid

#99515

Purpose

C-terminal AID* degron cassette with HygR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-114) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

Expression

Mutation

internal inversion of nucleotides 265-312 of the …

Promoter

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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