Addgene

We narrowed to 3,331 results for: Ubc

Showing: 761 - 780 of 3331 results

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pTBL3478 PB-PEmax-mTagBFP2

Plasmid

#226664

Purpose

PiggyBac cargo vector encoding PEmax and mTagBFP2

Depositor

Chang Liu

Article

Loveless et al bioRxiv 2021.11.05.467507

Inserts

PEmax
mTagBFP2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAG and UBC

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pLJC5-LAMP1-RFP-2xFlag

Plasmid

#102931

Purpose

Express a lysosomal Tag

Depositor

David Sabatini

Article

Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

Insert

LAMP1

Use

Lentiviral

Tags

mRFP-2xFlag

Expression

Mutation

Promoter

ubc

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pFudio-Cpg15-IRES2-TdTomatoW

Plasmid

#134309

Purpose

Expresses cpg15 and TdTomato upon coexpression with Cre recombinase

Depositor

Elly Nedivi

Article

Subramanian et al Cell Rep. 2019 Aug 6;28(6):1584-1595.e5.

Inserts

cpg15 (Nrn1 Rat, Mouse)
TdTomato

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

UbC

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pEYFP-C3-HA-Ubiquitin

Plasmid

#28236

Depositor

Michael Mancini

Article

Stenoien et al Nat Cell Biol. 2002 Oct . 4(10):806-10.

Insert

Ubiquitin (UBB Human)

Use

Tags

EYFP and HA

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2011

Availability

Academic Institutions and Nonprofits only

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pUB6-VSV-GS

Plasmid

#190781

Purpose

Expresses VSV-GS in mammalian cells

Depositor

Jakob Reiser

Article

Zhang et al Retrovirology. 2010 Jan 25;7:3. doi: 10.

Insert

VSV-GS

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

UbC

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLJC6-NADK-3xFLAG

Plasmid

#163452

Purpose

lentiviral overexpression vector for human NADK

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

NADK (NADK Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

See: comments

Promoter

Ubc

Available since

March 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCHA1.1-Rap2a-V5

Plasmid

#149509

Purpose

Stable expression of human Rap2a.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

RAP2 (RAP2A Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Codon Optimized Sequence

Promoter

Ubc

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pET23a-MmRPS27a

Plasmid

#69559

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

RPS27A (RPS27A Mouse, Human)

Use

Tags

Expression

Bacterial

Mutation

Changed Alanine 133 (human RPS27a) to Glycine (mo…

Promoter

T7

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
PumA

Plasmid

#87319

Purpose

PumHD w/each unit mutated to bind A (as in Fig. 1C; unit 6 is the Pumby for A)

Depositor

Edward Boyden

Article

Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

Insert

Pumilio protein mutant (PUM1 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

all 8 units mutated to bind A

Promoter

UBC

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pLJC5-Rap2A-3xFLAG

Plasmid

#163444

Purpose

lentiviral overexpression vector for human Rap2A

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

Rap2A (RAP2A Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Ubc

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLJC6-Rap2A-3xFLAG

Plasmid

#163445

Purpose

lentiviral overexpression vector for human Rap2A

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

Rap2A (RAP2A Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Ubc

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pDG279

Plasmid

#85219

Purpose

mammalian expression of ubiquitin-K48R-EGFP fusion

Depositor

Douglas Gray

Article

Tsirigotis et al J Biol Chem. 2001 Dec 7;276(49):46073-8.

Insert

ubiquitin C (UBC Human)

Use

Tags

6xHIS and EGFP

Expression

Mammalian

Mutation

K48R

Promoter

human ubiquitin C

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only
pLJC6-NADK (D184A)-3xFLAG

Plasmid

#233894

Purpose

lentiviral overexpression vector NADK (D184A)

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

NADK (NADK Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

D184A

Promoter

Ubc

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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FUGW-RUSH-Strep-KDEL

Plasmid

#228527

Purpose

Lentiviral vector, cloning vector for Strep-KDEL-IRES in mammalian cells.

Depositor

Ginny Farias

Article

Li et al Nat Commun. 2024 Dec;15: 10829. doi: 10.

Insert

Strep-KDEL

Use

Lentiviral

Tags

Streptavidin-KDEL

Expression

Mammalian

Mutation

Promoter

Ubc

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pUB_SM-FLuc0-GFPOPT

Plasmid

#210594

Purpose

Expresses SM(FLAG)-nonoptimal Firefly luciferase-optimal GFP

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-nonoptimal FLuc-Optimal GFP

Use

Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Mutation

Promoter

UBC human

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUB_SM-Fluc100-GFPOPT

Plasmid

#210596

Purpose

Expresses SM(FLAG)-optimal Firefly luciferase-optimal GFP

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-optimal FLuc-Optimal GFP

Use

Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Mutation

Promoter

UBC human

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUB_SM-RLuc0---MS2

Plasmid

#210572

Purpose

Expresses SM(FLAG)-nonoptimal Renilla with 3'UTR MS2 hairpins

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-nonoptimal Renilla luciferase

Use

Lentiviral and Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Mutation

Promoter

UBC human

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUB_SM-RLuc100---MS2

Plasmid

#210573

Purpose

Expresses SM(FLAG)-optimal Renilla with 3'UTR MS2 hairpins

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-optimal Renilla luciferase

Use

Lentiviral and Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Mutation

Promoter

UBC human

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET23a-MmUBB(SnaBI)

Plasmid

#69558

Purpose

In vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBB (Ubb Mouse)

Use

Tags

Expression

Bacterial

Mutation

The two last codons (TATTAA; encoding the last ty…

Promoter

T7

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pET23a-MmUBB(Y305P)

Plasmid

#69557

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBB (Ubb Mouse)

Use

Tags

Expression

Bacterial

Mutation

Codon TAT encoding tyrosine 305 was changed to CC…

Promoter

T7

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only