We narrowed to 5,504 results for: pcas

1. pCAG-p21_T145D

   Plasmid

   #40204

   Depositor

   Philipp Mergenthaler

   Article

   Mergenthaler et al J Cereb Blood Flow Metab. 2013 Mar;33(3)

   Insert

   p21 (CDKN1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T145D

   Promoter

   CAG

   Available since

   March 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAMTA2-donor

   Plasmid

   #132501

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   CAMTA2 (CAMTA2 Human)
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mutation

   none

   Promoter

   Available since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS_m-AKAR5’

   Plasmid

   #114140

   Purpose

   Mammalian expression of the AKAR5’ sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM

   Depositor

   Haining Zhong

   Article

   Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

   Insert

   AKAR5' C-terminally tagged by KRas C tail
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAH-MIBP16

   Plasmid

   #51870

   Purpose

   Expresses C-terminal HA-tagged full length rat MIBP1 (cloned into pCAGGS)

   Depositor

   Kenshi Hayashi, Tomoko Tahira

   Article

   Iwashita et al J Biol Chem. 2012 Mar 23;287(13):9887-90

   Insert

   MIBP1 (Hivep2 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pCAD96_hIRG1_4-461_pvp008

   Plasmid

   #124874

   Purpose

   Expresses hCAD Asn152Ser mutant in E.coli

   Depositor

   Konrad Buessow

   Article

   Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

   Insert

   cis-aconitate decarboxylase (ACOD1 Human)
   

   Use

   Tags

   StrepTag and TEV site

   Expression

   Bacterial

   Mutation

   mutated Asn152 to Ser, deleted amino acids 1-3 an…

   Promoter

   T7

   Available since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Topo_SpCas9.tracr_AsCas12a.DR

   Plasmid

   #155050

   Purpose

   TOPO vector for the cloning of _the SpCas9 tracrRNA - AsCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   SpCas9 tracrRNA and AsCas12a Direct Repeat (DR)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCALNL_chr13_102010574-102010650

   Plasmid

   #81213

   Purpose

   pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 13 (numbers in common name refers to pos. in hg38)

   Depositor

   David Liu

   Article

   Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

   Insert

   gix-Neo-gix deletion cassette upstream of EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCBa

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. Gli3ZF in AINGpCAGGS

   Plasmid

   #121972

   Purpose

   Gli3 zinc fingers

   Depositor

   James Briscoe

   Insert

   Gli3ZF (GLI3 Human)
   

   Use

   Chick electroporation

   Tags

   5xmyc

   Expression

   Mammalian

   Mutation

   N terminus and C terminus deletion only nt 1411-1…

   Promoter

   Available since

   April 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-EHC

   Plasmid

   #112619

   Purpose

   expression plasmid with CAG promoter driving multicistronic cassette H2BEmerald_p2A_HygroR_p2A_CreERt2

   Depositor

   Stefan Heller

   Article

   Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

   Inserts

   H2B-Emerald (H2BC3 Human, Aequorea victoria)
   Hygrophosphotransferase
   Cre-ERT2 fusion protein
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCALNL_Ch12_62418577-62418652

    Plasmid

    #81212

    Purpose

    pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 12 (numbers in common name refer to pos. in hg38)

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    gix-Neo-gix deletion cassette upstream of EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCBa

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBW700_pCAG-pDest-U1-UX

    Plasmid

    #87578

    Purpose

    pCAG destination vector (U1-UX)

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBluescript_VEGFA-TS3_SpCas9

    Plasmid

    #107328

    Purpose

    U6 driven SpCas9 sgRNA expression for VEGFA site 3

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    VEGFA-TS3 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCALNL-NaChBac

    Plasmid

    #125580

    Purpose

    Cre-dependent expression of NaChBac (FLAG-tagged) in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    NaChBac
    

    Use

    Cre/Lox

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAE-MIBP1

    Plasmid

    #51873

    Purpose

    Expresses full length rat MIBP1 (cloned in pCAGGS)

    Depositor

    Kenshi Hayashi, Tomoko Tahira

    Article

    Fukuda et al J Biochem. 2002 Mar;131(3):349-57.

    Insert

    MIBP1 (Hivep2 Rat)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pCAG-TALEN-A-pA

    Plasmid

    #45955

    Depositor

    Ralf Kuehn

    Insert

    TALEN
    

    Use

    Tags

    FokI nuclease domain

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-TALEN-T-pA

    Plasmid

    #45963

    Depositor

    Ralf Kuehn

    Insert

    TALEN
    

    Use

    Tags

    FokI nuclease domain

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-TALEN-G-pA

    Plasmid

    #45962

    Depositor

    Ralf Kuehn

    Insert

    TALEN
    

    Use

    Tags

    FokI nuclease domain

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcasper4 p [8-Gr66a+]

    Plasmid

    #26862

    Depositor

    Craig Montell

    Article

    Moon et al Curr Biol. 2006 Sep 19. 16(18):1812-7.

    Insert

    Gr66 gustatory receptor (Gr66a Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Contains Gr66a and neighboring genes CG7066 and C…

    Promoter

    Available since

    May 18, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pBW702_pCAG-PV2

    Plasmid

    #87562

    Purpose

    Simple PV2

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBW701_pCAG-PV1

    Plasmid

    #87561

    Purpose

    Simple PV1

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge