We narrowed to 18,339 results for: Mut
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Plasmid#87429PurposeHomology arms and linker-mEGFP sequence for N-terminus tagging of human TJP1DepositorInsertTJP1 Homology Arms with mEGFP-linker (TJP1 Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable SinceMarch 21, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHAGE-PTEN
Plasmid#116780PurposeLentiviral expression of PTENDepositorAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC-VIOL
Plasmid#53087PurposeProduces the carotenoid violaxanthin in E. coli.DepositorInsertzep (ABA1 Mustard Weed)
UseLow copy number bacterial cloning vectorTagsExpressionMutationLacks codons for the first 60 N-terminal amino ac…PromoterT7Available SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
A24M04 mIgM heavy chain-pFEEKW
Plasmid#234809PurposeExpress membrane form of IgM heavy chain containing the VH region of the monoclonal antibody, A24M04, specific for human papilloma virus16 L1DepositorInsertA24M04 membrane IgM heavy chain (IGHM Human)
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterEEK promoterAvailable SinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSN840
Plasmid#184832PurposeExpresses human KIF5A(∆exon27) and human KLC1 in insect cellsDepositorUseCre/LoxTagsHis-FLAG and mScarlet-2xStrepIIExpressionInsectMutation∆exon27 form of human KIF5APromoterp10 and polyhedrinAvailable SinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
A24M04 Ig kappa light chain-pFEEKW
Plasmid#234810PurposeExpress Ig kappa light chain containing the VL region of the monoclonal antibody, A24M04, specific for human papilloma virus16 L1DepositorUseLentiviralTagsExpressionBacterial and MammalianMutationPromoterEEK promoterAvailable SinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBFC0984
Plasmid#231992PurposeCRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with 2xBsaI spacer cloning siteDepositorInsertsdRfxCas13d
crRNA with 2xBsaI spacer
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119 and pTetAvailable SinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBOB-CAG-SARS-CoV2-Spike-HA
Plasmid#141347Purpose3rd Generation lentiviral vector expressing the codon-optimized SARS-CoV2 Spike Glycoprotein ORF with a C-terminal HA tag generated by Junko Ogawa & Gerald M PaoDepositorInsertSARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
UseLentiviralTagsHAExpressionMutationresynthesized with human codon optimization nucle…PromoterCAGAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-hSNCA
Plasmid#170445PurposeLentiviral vector expressing human SNCA, under control of EF1alpha promoter.DepositorAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBFC0993
Plasmid#231993PurposeCRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with negative control (RFP-targeting) spacerDepositorInsertsdRfxCas13d
crRNA with negative control (RFP-targeting) spacer
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119 and pTetAvailable SinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-Flag-NLRP3 1-90
Plasmid#75137PurposeExpresses N-terminal Flag-tagged amino acids 1-90 of NLRP3 (the pyrin domain) in mammalian cellsDepositorInsertNLR family, pyrin domain containing 3 (Nlrp3 Mouse)
UseTagsFlagExpressionMammalianMutationfragment encoding amino acids 1-90PromoterCMVAvailable SinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pU6-ccdB-boxB-tBE-V5-mA3
Plasmid#171693PurposeExpresses tBE-V5-mA3 in mammalian cellsDepositorInsertccdB-sgRNA scaffold-boxB-tBE-V5-mA3
UseCRISPRTagsExpressionMammalianMutationPromoterU6 and EF1aAvailable SinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHEE401
Plasmid#71286PurposeEgg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistanceDepositorInsertssgRNA scaffold
zCas9
UseCRISPR; Plant binary vectorTags3x FLAG and NLSExpressionPlantMutationZea mays codon-optimized Cas9PromoterEC1.2 promoter and U6-26p Arabidopsis U6 gene pro…Available SinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-puro /BRAF-V600E
Plasmid#131724PurposeMammalian Expression of Flag tagged BRAFDepositorInsertBRAF (BRAF Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationV600EPromoterCMVAvailable SinceFeb. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-Flag-ASC1
Plasmid#75134PurposeExpresses N-terminal Flag-tagged ASC1 in mammalian cellsDepositorInsertPYD and CARD domain containing (Pycard Mouse)
UseTagsFLAGExpressionMammalianMutationF23S (please see depositor comments below)PromoterCMVAvailable SinceAug. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lenti-RNF2-ngRNA+41_EF1a-puroR
Plasmid#207358PurposeLentiviral transfer plasmid encoding hU6-driven expression of a RNF2 nicking guide RNA and EF1a-driven puromycin resistance gene.DepositorInsertRNF2 +41 ngRNA
UseLentiviralTagsExpressionMutationPromoterAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-TEV-KRAS
Plasmid#111849PurposeExpresses wild type human KRAS (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS (KRAS Human)
UseTags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationPromoterT5Available SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBFC1171
Plasmid#231994PurposeCRISPRi-ART crRNA only plasmid encoding crystal violet-inducible crRNA (for dRfxCas13d) with 2xBsaI spacer cloning siteDepositorInsertcrRNA of dRfxCas13d
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterpJEx (Jungle Express)Available SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 Nucleocapsid-3xHA-IRES-mcherry
Plasmid#173030PurposeTo express SARS-CoV-2 Nucleocapsid protein tagged with 3xHA and in frame with mcherry (NCP-IRES-mcherry) in mammalian cellsDepositorInsertSARS-CoV-2 Nucleocapsid phosphoprotein
UseLentiviralTags3xHA and IRES-mcherryExpressionMammalianMutationPromoterGag (truncated)Available SinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_S100A13_WT_V5
Plasmid#82975PurposeGateway Donor vector containing S100A13, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only