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pBbdCas9S_P0526-sgRNAmreB

Plasmid

#149654

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi mreB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAmreB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0526

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-Gal4

Plasmid

#62900

Purpose

Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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Spy CCR5-pegRNA

Plasmid

#169856

Purpose

SpyCas9-pegRNA for CCR5

Depositor

Scot Wolfe

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

Spy CCR5-pegRNA

Expression

Mammalian

Promoter

human U6 promoter

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-Gal4

Plasmid

#62901

Purpose

Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pRNA-U6-let7 sponge

Plasmid

#35664

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

U6 let-7sponge

Promoter

U6

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only

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pRRL-VinculinTS-A50I

Plasmid

#111831

Purpose

Vinculin tension sensor with point mutation affecting ability to bind talin in lentiviral expression vector

Depositor

Brenton Hoffman

Article

Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

Insert

VinculinTS (A50I) (VCL Chicken)

Use

Lentiviral

Mutation

changed vinculin alanine 50 to isoleucine

Promoter

CMV

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-FLEX.iGABASnFR

Plasmid

#112166

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR

Use

AAV

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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IQ013: pMVP/Ad-DEST

Plasmid

#121843

Purpose

pMVP destination vector, empty adenovirus vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Adenoviral; Pmvp gateway destination vector

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pmRFP-SUMO1 GG

Plasmid

#13990

Depositor

Jonathan Chernoff

Article

Dadke et al Nat Cell Biol. 2007 Jan . 9(1):80-85.

Insert

SUMO1 GG (Sumo1 Mouse)

Tags

mRFP

Expression

Mammalian

Mutation

C-terminal truncation. Cannot ligate to substrate…

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
pmScarlet-i_NES_C1

Plasmid

#85062

Purpose

In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

NES

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-GFP-2A-oG-WPRE

Plasmid

#102984

Purpose

Lentivirus expressing eGFP and optimised Rabies Glycoprotein (oG)

Depositor

Marco Tripodi

Article

Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

Inserts

Enhanced Green Fluorescent Protein (eGFP)
Optimised Rabies glycoprotein (oG)

Use

Lentiviral

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-mTIP49a

Plasmid

#15357

Depositor

Joan Conaway, Ronald Conaway

Article

Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

Insert

TATA binding protein interacting protein 49 kDa (Ruvbl1 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
actc1b-mKate2-CaaX(tK)

Plasmid

#109488

Purpose

Muscle specific zebrafish expression of mKate2 fused to the CaaX domain from Kras

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b-mKate2-CaaX(tK)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ1201

Plasmid

#44483

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, unc-119, peel-1, MCS

Use

Multiple cloning site vector

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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IF101: pMVP (L1-L4) EF1a promoter

Plasmid

#121687

Purpose

pMVP L1-L4 entry plasmid, contains EF1a promoter for 3-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

EF1a promoter

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCFJ1272

Plasmid

#44486

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, MCS

Use

Multiple cloning site vector

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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HB401: pMVP (L1-L4) CMV promoter

Plasmid

#121686

Purpose

pMVP L1-L4 entry plasmid, contains CMV promoter for 3-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

CMV promoter

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2 3xmiR-122T

Plasmid

#35672

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

3 miR-122 target sites

Use

Luciferase

Promoter

HSV-TK

Available Since

April 13, 2012

Availability

Academic Institutions and Nonprofits only

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HA-hAtg13

Plasmid

#31967

Depositor

Do-Hyung Kim

Article

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

Atg13 (ATG13 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only
pTX1-SMN1-exon7-ESE1

Plasmid

#126041

Purpose

In-vitro-transcription of SMN1-exon7-ESE1 RNA in NMR tube for "Systems NMR" analysis

Depositor

Frédéric Allain

Article

Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

Insert

SMN1 exon7 ESE1

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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