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pLenti CRISPR sgSHMT1_2

Plasmid

#106312

Purpose

Express Cas9 and sgRNA targeting SHMT1

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting SHMT1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCFJ2210 - [1-2] ENTR - Fluor - mMaple3(1 intron, syntrons(3), no_start, no_stop)

Plasmid

#159864

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - mMaple3(1 intron, syntrons(3), no_start, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_SLA2-1/1

Plasmid

#91450

Purpose

Protein expression and purification of human SH3 domain construct SLA2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SLA2-1/1 (SLA2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_NCK2-2/3

Plasmid

#91362

Purpose

Protein expression and purification of human SH3 domain construct NCK2-2/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NCK2-2/3 (NCK2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pMS7: pCascade(AvCAST)_entry

Plasmid

#168140

Purpose

Inducible expression of AvCAST Cascade proteins. Two BsaI sites for spacer cloning.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST minimal CRISPR array
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV Aquamarine-shadowY tandem

Plasmid

#157775

Purpose

Produces a fusion between Aquamarine and shadowY. It can be used as a positive control for FRET (using FLIM)

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Type

Empty backbone

Tags

Aquamarine and shadowY

Expression

Mammalian

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pHL 651

Plasmid

#53016

Purpose

LacIq + PCon::RBS (st3) tetR::T1 terminator + p15a + CamR. Backbone from pZA32MCS1 (Lutz and Bujard, 1997).

Depositor

Han Lim

Article

Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

Inserts

LacIq
tetR

Use

Synthetic Biology

Tags

RBS(st3) and T1 terminator

Expression

Bacterial

Promoter

pCon

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
LjUBQ:MtGLV6:T35s

Plasmid

#164741

Purpose

Overexpression of Peptide coding gene (Binary vector)

Depositor

Wolf Scheible

Article

Roy et al Plant J. 2024 May;118(3):607-625. doi: 1

Insert

Medtr3g095180.1

Tags

Terminator 35S

Expression

Plant

Promoter

Lotus japonicus Ubiquitin

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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LjUBQ:MtGLV2:T35s

Plasmid

#164740

Purpose

Overexpression of Peptide coding gene (Binary vector)

Depositor

Wolf Scheible

Article

Roy et al Plant J. 2024 May;118(3):607-625. doi: 1

Insert

Medtr5g012400.1

Tags

Terminator 35S

Expression

Plant

Promoter

Lotus japonicus Ubiquitin

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO - RBM28 E5 WT minigene

Plasmid

#169269

Purpose

Mammalian vector for constitutive expression of RBM28 Exon 5 WT minigene

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 E5 WT minigene (RBM28 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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