We narrowed to 8,554 results for: AMPH
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Plasmid#178981PurposepACYC Duet-1 containing full Type III-E system Candidatus “Scalindua brodae” CRISPR arrayDepositorInsertCRISPR array from Candidatus Scalindua brodae
ExpressionBacterialPromoterT7Available SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
p2xp53_RE
Plasmid#118053PurposeGBPart - Operator/DNA Response element - 2 copies of the p53 DNA binding motifDepositorInsert2 copies of the p53 DNA binding motif
UseSynthetic Biology; Domestication of dna parts for…Available SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNSSCPG
Plasmid#183187PurposePACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; sfGFP-LAA expressed under T7 pDepositorInsertssuperfolder GFP
SpmX450 and T7 181+ fusion protein
T7 RNAP 1-179 variant and SpmX450 fusion protein
mRFP1 and popZ fusion protein
ExpressionBacterialAvailable SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAGE2.0-i
Plasmid#194639PurposeEmpty pAGE2.0 plasmid with pBAD inducible promoterDepositorTypeEmpty backboneUseSynthetic Biology; Diatom expressionExpressionBacterial and YeastAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIN4_tsp-6_mScarlet
Plasmid#182935PurposeExpresses tsp-6::mScarlet under its native promoter (amphid and phasmid ciliated neurons in C. elegans)DepositorInsertPtsp-6-3::tsp-6::flexible linker::mScarlet (C. elegans, genomic)
Tagsflexible linker (GGGGS)x3 and mScarlet tagExpressionWormPromotertsp-6Available SinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
N-BLa 1.1
Plasmid#88994PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the N-terminal fragment of the split β-lactamase.DepositorInsertN-BLa 1.1 fusion protein
TagsFlagExpressionBacterialPromoteraraBADAvailable SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
Keasling-2187
Plasmid#100167PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growthDepositorInsertMevT and MBIS operon
ExpressionBacterialPromoterlacUV5Available SinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pINDgw RFA-V5-EcoDam
Plasmid#59204PurposeMammalian DamID vector for fusing gene of interest with Dam-V5 using Gateway cloningDepositorTypeEmpty backboneUseDamidTagsDam (DNA adenine methyltransferase) and V5ExpressionMammalianPromoterHeat Shock Minimal PromoterAvailable SinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pTU425
Plasmid#178984PurposepACYC containing Candidatus “Scalindua brodae” TPR-CHAT H585A C627A (codon-optimized)DepositorInsertTPR-CHAT H585A C627A
TagsHis-tagExpressionBacterialPromoterT7Available SinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC010 LshCas13a locus with nontargeting spacer
Plasmid#91901PurposeLshCas13a locus into pACYC184 with nontargeting spacerDepositorInsertLshCas13a locus with non-targeting spacer
ExpressionBacterialAvailable SinceOct. 16, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCK389.AAV
Plasmid#177327PurposeaTc inducible MCP-SoxS double mutant expression for CRISPRa in E.coli. Contains constitutive dCas9 and scRNA expressionDepositorInsertsSoxS
dCas9
UseCRISPRTagsMCP (MS2 Coat Protein)MutationR93A,S101AAvailable SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSR284
Plasmid#125093PurposeExpression of ompR(1-122aa)-manR(120-234aa) chimera under PLtetO-1, output Promoter PmntCAB143DepositorInsertsompR(REC)-manR(DBD)122
sfgfp
tetR
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD279
Plasmid#125099PurposeExpression of SO_4388(1-130aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110DepositorInsertsSO_4388(REC)-psdR(DBD)137
sfgfp
tetR
UseSynthetic BiologyExpressionBacterialAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAVA-LGF2-Gal11p
Plasmid#127481PurposePositive control for the AVA-seq system. LGF2 (lambda CI associated) and Gal11p (RNAp associated) interactionDepositorInsertLGF2-Gal11p (GAL11 Budding Yeast)
ExpressionBacterialAvailable SinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_RPS3_K214R
Plasmid#127132DepositorAvailable SinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR241
Plasmid#125091PurposeExpression of ompR(1-137aa)-yxdJ(132-229aa) chimera under PLtetO-1, output Promoter PyxdL181DepositorInsertsompR(REC)-yxdJ(DBD)137
sfgfp
tetR
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
Bin1 N-BAR domain
Plasmid#168280PurposeExpress N-BAR domain of Bin1 (aa 1-267) in pEGFP-N1 vectorDepositorAvailable SinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-151
Plasmid#118533PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmTurquoise2
UseMinitn5 delivery vectorExpressionBacterialAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHS1068
Plasmid#205962PurposeNZ_CP025815 DinG HNH proteins (E. coli codon optimized) and CRISPR array in pACYCDuet-1 with Lac promotersDepositorInsertDinG HNH proteins and CRISPR array
ExpressionBacterialPromoterLac (proteins), pJ23119 (crRNA)Available SinceFeb. 2, 2024AvailabilityAcademic Institutions and Nonprofits only