We narrowed to 18,339 results for: Mut

1. pCMV-HA_N-human Prkd2 without kinase domain

   Plasmid

   #138411

   Purpose

   Express Prkd2 without kinase domain in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Misawa et al Sci Immunol. 2020 Jan 24;5(43):eaaz0085.

   Insert

   Prkd2 without kinase domain (PRKD2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   kinase domain deleted from human Prkd2

   Promoter

   CMV

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCW-ND-Cdt1ΔR198A/R210A-HA-Puro

   Plasmid

   #193769

   Purpose

   Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS , C-terminal HA ) w/ mutations stopping S phase degradation and in MCM binding (R198A/R210A), expressed from all-in-one pCW vector (TetOn + rtTA)

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   ND-Cdt1ΔR198A/R210A (CDT1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…

   Promoter

   TRE

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. hKCNQ5(WT).ires.GFP-pcDNA3

   Plasmid

   #204360

   Purpose

   Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

   Depositor

   Aguan Wei

   Article

   Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

   Insert

   KCNQ5 (KCNQ5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/T7 RNApol

   Available Since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX304 ERG R367K

   Plasmid

   #118626

   Purpose

   Overexpresses ERG R367K (DNA binding mutant) protein

   Depositor

   William Hahn

   Article

   Sandoval et al Mol Cell. 2018 Aug 16;71(4):554-566.e7.

   Insert

   ERG ( R367K DNA Binding mutant) (ERG Human)
   

   Use

   Lentiviral

   Tags

   None

   Expression

   Mammalian

   Mutation

   R367K

   Promoter

   CMV

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHIV-EGFP.Flag-EZH2-mt2

   Plasmid

   #220245

   Purpose

   Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

   Depositor

   Chen Davidovich

   Article

   Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

   Insert

   EZH2 mt 2 (EZH2 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

   Promoter

   EF-1α

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. V5-ERG-R367K

   Plasmid

   #118624

   Purpose

   Expresses V5 tagged ERG R367K (DNA binding mutant) protein

   Depositor

   William Hahn

   Article

   Sandoval et al Mol Cell. 2018 Aug 16;71(4):554-566.e7.

   Insert

   ERG (R367K DNA binding mutant) (ERG Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   R367K

   Promoter

   EF1a

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE-TGFBR2

   Plasmid

   #116800

   Purpose

   Lentiviral expression of TGFBR2

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   TGFBR2 (TGFBR2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Ef1a-pspCas13b-NES-3xFLAG-T2A-BFP

   Plasmid

   #173029

   Purpose

   For expression of cytoplasmic pspCas13b protein tagged with 3xHA-T2A-BFP for gene silencing in mamallian cells.

   Depositor

   Mohamed Fareh

   Article

   Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:

   Insert

   pspCas13b
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG, BFP, HIV NES, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5-FRT/TO-APT2-LaG16-3W

   Plasmid

   #234425

   Purpose

   Express APT2 with a mutated non-binding anti-GFP nanobody LaG16 (F30W, T55W, G103W) fused at the C terminus separated by a flexible linker

   Depositor

   William Fuller

   Article

   Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:

   Insert

   APT2 fused to non-binding mutant LaG16 (LYPLA2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5-FRT/TO-APT1-LaG16-3W

    Plasmid

    #234424

    Purpose

    Express APT1 with a mutated non-binding anti-GFP nanobody LaG16 (F30W, T55W, G103W) fused at the C terminus separated by a flexible linker

    Depositor

    William Fuller

    Article

    Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:

    Insert

    APT1 fused to non-binding mutant LaG16 (LYPLA1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGMC00005

    Plasmid

    #166722

    Purpose

    sgRNA against human ASNS

    Depositor

    Raj Chari

    Article

    Ruiz-Rodado et al Cancer Metab. 2020 Oct 21;8:23. doi: 10.

    Insert

    ASNS (ASNS Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGMC00006

    Plasmid

    #166723

    Purpose

    sgRNA against human ASNS

    Depositor

    Raj Chari

    Article

    Ruiz-Rodado et al Cancer Metab. 2020 Oct 21;8:23. doi: 10.

    Insert

    ASNS (ASNS Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-Tet-MKK6(DD)-Puro

    Plasmid

    #86094

    Purpose

    Tet/Dox inducible (TetR) constitutively active mutant MKK6/MAP2K6 in lentiviral vector

    Depositor

    Cindy Miranti

    Article

    Frank et al J Cell Sci. 2017 Apr 26. pii: jcs.197152

    Insert

    MKK6 (MAP2K6 Human)
    

    Use

    Lentiviral

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Serine 207 and Threonine 211 both changed to Aspa…

    Promoter

    CMV/TetO

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH-CMV-hLamin_A-IRES-copGFP-EF1-puro

    Plasmid

    #132773

    Purpose

    Expression of human LMNA.

    Depositor

    Jan Lammerding

    Article

    Earle et al Nat Mater. 2019 Dec 16. pii: 10.1038/s41

    Insert

    Lamin A (LMNA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. FRIG-myc-Sema4D-CD4

    Plasmid

    #51606

    Purpose

    expresses myc-tagged Sema4D/CD4 non-cleavable chimera with extracellular Sema4D, intracellular CD4 in lentiviral backbone

    Depositor

    Suzanne Paradis

    Article

    Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

    Insert

    Sema4D/CD4-FLAG (Sema4d Human, Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG and myc

    Expression

    Mammalian

    Mutation

    Sema4D amino acids 660-861 were replaced with CD4…

    Promoter

    RSV

    Available Since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pLenti-puro/RAF1

    Plasmid

    #131725

    Purpose

    Mammalian Expression of RAF1

    Depositor

    Jacques De Grève

    Article

    Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

    Insert

    RAF1 (RAF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A

    Plasmid

    #51248

    Purpose

    Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907,980,1006 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli3 (Gli3 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV and Flag

    Expression

    Mammalian

    Mutation

    amino acids 849,865,877,907,980,1006 mutated to A…

    Promoter

    EF1a

    Available Since

    March 7, 2014

    Availability

    Academic Institutions and Nonprofits only

18. hKCNQ3(WT).ires.mCherry-pcDNA3

    Plasmid

    #204362

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    KCNQ3 (KCNQ3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/T7 RNApol

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-HA_N-mouse Bcl6

    Plasmid

    #138410

    Purpose

    Express mouse Bcl6 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Misawa et al Sci Immunol. 2020 Jan 24;5(43):eaaz0085.

    Insert

    B cell lymphoma 6 (Bcl6 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHAGE-PDGFRB

    Plasmid

    #116770

    Purpose

    Lentiviral expression of PDGFRB

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PDGFRB (PDGFRB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge