We narrowed to 12,154 results for: NSI;

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pHH0103_LASP1-1/1-SV1/2

Plasmid

#91399

Purpose

Protein expression and purification of human SH3 domain construct LASP1-1/1-SV1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

LASP1-1/1-SV1/2 (LASP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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PX459v2-ILK-gRNA 1-exon 1

Plasmid

#163320

Purpose

SpCas9 ILK gRNA 1 (G*CGGAGAACGACCTCAACCAG), targeting exon 1 within the N-terminal ankyrin repeat domain-1.

Depositor

Val Brunton

Article

Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

Insert

ILK gRNA 1_Exon1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only
PX459v2-ILK-gRNA 2-exon 8

Plasmid

#163321

Purpose

SpCas9 ILK gRNA 2 (G*ACATTGTAGAGGGATCCATA), targeting exon 8 within the C-terminal protein kinase domain.

Depositor

Val Brunton

Article

Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

Insert

ILK gRNA 2_Exon8

Use

CRISPR

Expression

Mammalian

Promoter

U6F

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only
TVBB C-term-Dendra2

Plasmid

#169216

Purpose

Targeting vector backbone to support a knock-in of Linker-Dendra2 at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked Dendra2-P2A-Puro

Use

Targeting vector backbone

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_GRB2-2/2

Plasmid

#91410

Purpose

Protein expression and purification of human SH3 domain construct GRB2-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

GRB2-2/2 (GRB2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_GRAP2-2/2

Plasmid

#91368

Purpose

Protein expression and purification of human SH3 domain construct GRAP2-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

GRAP2-2/2 (GRAP2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_EPS8L2-1/1

Plasmid

#91526

Purpose

Protein expression and purification of human SH3 domain construct EPS8L2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

EPS8L2-1/1 (EPS8L2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pET-45b-RUNX2(+10A)-IDR-mCherry

Plasmid

#145287

Purpose

Bacterial Expression of 6xHis RUNX2(+10A) IDR mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

RUNX2(+10A)-IDR (RUNX2 Human)

Expression

Bacterial

Mutation

A-repeat expanded

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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C-His-GvpC-Calpain

Plasmid

#153295

Purpose

Expresses calpain-sensitive variant of Anabaena flos-aquae GvpC in E.coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

Insert

Calpain-sensitive Gas vesicle protein C (his-tagged)

Tags

His-tag

Expression

Bacterial

Mutation

1) Contains an extra glycine after the methionine…

Promoter

T7

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_CRK-2/2-SV2/2

Plasmid

#91343

Purpose

Protein expression and purification of human SH3 domain construct CRK-2/2-SV2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CRK-2/2-SV2/2 (CRK Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SRC-1/1-IS1/2

Plasmid

#91349

Purpose

Protein expression and purification of human SH3 domain construct SRC-1/1-IS1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SRC-1/1-IS1/2 (SRC Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 2xFLAG-2xSTREP EMI1

Plasmid

#158947

Purpose

Express human EMI1 in mammalian cells

Depositor

Michele Pagano

Article

Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

Insert

EMI1 (FBXO5 Human)

Tags

2X FLAG 2X STREP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-EOMESwt-IDR

Plasmid

#145231

Purpose

Expresses fusion of GAL4 DNA-binding domain and EOMESwt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

EOMESwt-IDR (EOMES Human)

Expression

Mammalian

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-BHLHE41wt-IDR

Plasmid

#145229

Purpose

Expresses fusion of GAL4 DNA-binding domain and BHLHE41wt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

BHLHE41wt-IDR (BHLHE41 Human)

Expression

Mammalian

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXA13wt-IDR

Plasmid

#145240

Purpose

Expresses fusion of GAL4 DNA-binding domain and HOXA13wt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXA13wt-IDR (HOXA13 Human)

Expression

Mammalian

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-158/+19

Plasmid

#35541

Depositor

Greg Goodall

Article

Bracken et al Cancer Res. 2008 Oct 1;68(19):7846-54.

Insert

promoter region of miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)

Use

Luciferase

Tags

luciferase

Promoter

none

Available Since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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pGL3-110/+19

Plasmid

#35542

Depositor

Greg Goodall

Article

Bracken et al Cancer Res. 2008 Oct 1;68(19):7846-54.

Insert

promoter region of miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)

Use

Luciferase

Tags

luciferase

Promoter

none

Available Since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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pHH0103_ASAP1-1/1

Plasmid

#91501

Purpose

Protein expression and purification of human SH3 domain construct ASAP1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ASAP1-1/1 (ASAP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 sgATXN1L-2

Plasmid

#74971

Purpose

sgATXN1L-2, puromycin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

ATXN1L (ATXN1L Human)

Use

CRISPR

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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LP696

Plasmid

#90177

Purpose

pEGFP-C1 with cDNA encoding HsKIF13B aa residues 1289-1826

Depositor

Lotte Pedersen

Article

Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

Insert

KIF13B (KIF13B Human)

Tags

GFP

Expression

Mammalian

Mutation

aa residues 1289-1826

Promoter

CMV

Available Since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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