We narrowed to 10,560 results for: fus
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Plasmid#159590Purposeexpresses Ufd4-flag in yeast cellsDepositorInsertUfd4
ExpressionYeastAvailable SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HT JS-Munc18c
Plasmid#135555PurposeExpress mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.DepositorInsertMunc18c
Tags6xHis tag and TEV siteExpressionInsectPromoterpolyhedrin promoterAvailable SinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mChe-Cs
Plasmid#183171PurposeConstruct for transient expression of cytoskeleton-targeted mCherry in plants.DepositorInsertmCherry-Cs
Tags363 aa of A. thaliana FIMBRIN 1 (AtFIM1)ExpressionPlantAvailable SinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB231
Plasmid#68622PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsEmerald Luc fused with PEST sequence [ELuc(PEST)]ExpressionPlantAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-Cx26
Plasmid#69021PurposeExpresses rat Cx26 (Gjb2 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.DepositorAvailable SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-GpNLuc
Plasmid#208840PurposeExpresses FLAG epitope-tagged GpNLuc "Green" BRET reporter in mammalian cells.DepositorInsertGpNLuc LumiFluor
UseLentiviralTags1xFLAGExpressionMammalianPromoterCMVAvailable SinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-FL-syt1
Plasmid#213628PurposeBacterial expression of SUMO-tagged rat FL-syt1DepositorAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-ChRmine-oScarlet-WPRE
Plasmid#183518PurposeOptogeneticsDepositorInsertChRmine-oScarlet
UseAAVPromoterCaMKIIaAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19/Pd-mChe
Plasmid#183172PurposeConstruct for transient expression of plasmodesmata-targeted mCherry in plants.DepositorInsertPd-mCherry
TagsAtPDCB1, A. thaliana PLASMODESMATA CALLOSE-BINDIN…ExpressionPlantAvailable SinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL001
Plasmid#115674PurposeFluorescent reporter for E.coli K12 fliC promoter activityDepositorInsertfliC promoter fused with GFP
TagsGreen fluorescent protein (GFP)ExpressionBacterialPromoterfliC promoterAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pASPIre3
Plasmid#154844PurposeDerivative of pASPIre2 lacking mCherry but containing a silent 150 bp DNA spacer flanked by attB/P sites; main uASPIre plasmid used in this studyDepositorInsertBxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked silent discriminator
ExpressionBacterialAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK376 (Hygro-P2A-mClover)
Plasmid#121187PurposeN-terminal taggingDepositorInsertHygro-P2A-mClover
UseCRISPRAvailable SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
YY171: pPB_NFκB::GFP-GEMINI(A)_TRE::HaloTag-GEMINI(A)_UbC::rtTA3-IRES-PuroR
Plasmid#228883PurposePiggyBac plasmid expressing the GFP-GEMINI(A) under the control of a synthetic NFκB-responsive promotor, and rtTA3 and PuroR under the control of a UbC promoter.DepositorInsertGFP-fused GEMINI(A), HaloTag-fuse GEMINI(A)
ExpressionMammalianPromoterpNFκB; TREAvailable SinceFeb. 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
PDE9A_pcDNA6.2/EmGFP-Bsd
Plasmid#176945PurposeMammalian expression vector encoding PDE9A and EmGFP-BsdDepositorAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
CFAP410_pcDNA6.2/EmGFP-Bsd
Plasmid#176948PurposeMammalian expression vector encoding CFAP410 and EmGFP-BsdDepositorAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cx43K258stop-msfGFP
Plasmid#69023PurposeExpresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged with msfGFP on the C-terminus. CMV promoter.DepositorInsertCx43 (Gja1 Rat)
TagsV206K monomerized super folder GFPExpressionMammalianMutationdelete codons 258-381 and fuse in frame to monome…PromoterCMVAvailable SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVHC_PGKneoLox2DTA.2
Plasmid#112622PurposeH2BVenus_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology armsDepositorInsertsUseCre/Lox and Mouse TargetingTagsVenusPromoterNo PromoterAvailable SinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
YFP(1-158)-beta-5 in pcDNAI/Amp
Plasmid#54470PurposeAn amino-terminal YFP fragment was fused to Gbeta-5. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.DepositorInsertYFP(1-158)/beta-5 (GNB5 Human, Aequorea victoria)
TagsYFP(1-158) was fused to the amino terminus of Gbe…ExpressionMammalianMutationYFP (1-158) includes a substitution of Met for Gl…Available SinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)
Plasmid#61364Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…PromoterCMVAvailable SinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-MinE-IRES-Blast
Plasmid#213115PurposeExpression of untagged MinE in mammalian cellsDepositorAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only