We narrowed to 8,835 results for: sgRNA

1. pInducer20 CARD8-HA PAM L368G

   Plasmid

   #169985

   Purpose

   Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (L368G) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   PAM, L368G

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pInducer20 CARD8-HA PAM R394E

   Plasmid

   #169987

   Purpose

   Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (R394E) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   PAM, R394E

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pInducer20 CARD8-HA PAM F405G

   Plasmid

   #169988

   Purpose

   Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F405G) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   PAM, F405G

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pInducer20 CARD8-HA PAM F370G

   Plasmid

   #169986

   Purpose

   Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F370G) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   PAM, F370G

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Direct-seq_lentiGuide-Puro-Tetra Loop-8A8G

   Plasmid

   #157982

   Purpose

   Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

   Depositor

   Lijia Ma

   Article

   Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

   Insert

   S. pyogenes sgRNA cassette (Tetra-8A8G)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV_COL4A3_self-cleavingCas9

   Plasmid

   #130281

   Purpose

   Expresses an spCas9 under the control of a CMV promoter; The spCas9 is flanked by COL4A3 sgRNA targets to induce its self-cleaving and inactivation

   Depositor

   Silvestro Conticello

   Article

   Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

   Insert

   spCas9
   

   Use

   AAV

   Promoter

   CMV

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV_COL4A5_self-cleavingCas9

   Plasmid

   #130282

   Purpose

   Expresses an spCas9 under the control of a CMV promoter; The spCas9 is flanked by COL4A5 sgRNA targets to induce its self-cleaving and inactivation

   Depositor

   Silvestro Conticello

   Article

   Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

   Insert

   spCas9
   

   Use

   AAV

   Promoter

   CMV

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-EN1680_Sororin-Cterm

   Plasmid

   #156451

   Purpose

   For transient expression of Cas9 and sgRNA targeting mouse Cdc5a (SORORIN) stop codon

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   Cas9, SORORIN (Cdca5 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiGuide-Puro-NCOA7g3 (BB24)

   Plasmid

   #139459

   Purpose

   Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a / U6

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSECC-sgSIK3-A

    Plasmid

    #138672

    Purpose

    Expresses a mouse SIK3-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK3 mouse (Sik3 Mouse)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSECC-sgSIK2-A

    Plasmid

    #138670

    Purpose

    Expresses a mouse SIK2-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK2 mouse (Sik2 Mouse)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSECC-sgSIK3-C

    Plasmid

    #138674

    Purpose

    Expresses a mouse SIK3-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK3 mouse (Sik3 Mouse)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSECC-sgSIK1-D

    Plasmid

    #138668

    Purpose

    Expresses a mouse SIK1-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK1 mouse (Sik1 Mouse)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSECC-sgSIK2-B

    Plasmid

    #138671

    Purpose

    Expresses a mouse SIK2-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK2 mouse (Sik2 Mouse)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSECC-sgSIK1-E

    Plasmid

    #138669

    Purpose

    Expresses a mouse SIK1-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK1 mouse (Sik1 Mouse)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSECC-sgSIK3-D

    Plasmid

    #138675

    Purpose

    Expresses a mouse SIK3-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK3 mouse (Sik3 Mouse)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. ARB366

    Plasmid

    #124049

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to KRAB domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::KRAB-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. ARB367

    Plasmid

    #124050

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to VPR domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::VPR-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTBL405 CHYRON1 integration construct

    Plasmid

    #126442

    Purpose

    To integrate the CHYRON1 locus at HEK293site3.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6-CHYRON1 hgRNA
    pCMV-puro
    

    Expression

    Mammalian

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    CMV and human U6

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLentiCMV-Blast mLST8-Flag Mut1

    Plasmid

    #124916

    Purpose

    For stable expression sgRNA resistant Flag tagging mLST8 Q44E/N46L mutant

    Depositor

    Jin Chen

    Article

    Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

    Insert

    MLST8 (MLST8 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Insert ORF was sirently mutated to be resistent t…

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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