Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 9,887 results for: Coli

Showing: 7861 - 7880 of 9887 results

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pUC57-mini v2-HF24_F10

Plasmid

#195724

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#10 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F6

Plasmid

#195720

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#6 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F15

Plasmid

#195729

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#15 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F17

Plasmid

#195731

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#17 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F19

Plasmid

#195733

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#19 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F2

Plasmid

#195716

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#2 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F11

Plasmid

#195725

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#11 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F16

Plasmid

#195730

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#16 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F18

Plasmid

#195732

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#18 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F1

Plasmid

#195715

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#1 of a 24 fragment split in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F9

Plasmid

#195723

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#9 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F23

Plasmid

#195737

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#23 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F21

Plasmid

#195735

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#21 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pET21a Sa-iPGM-bio

Plasmid

#179523

Purpose

Expresses S. aureus iPGM containing a C-terminal biotinylation site and His tag in E. coli

Depositor

James Inglese

Article

van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

Insert

S. aureus iPGM bio-6His

Tags

His tag and biotinylation sequence

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pET21a Mo-iPGM-bio

Plasmid

#179614

Purpose

Expresses M. orale iPGM containing a C-terminal biotinylation site and His tag in E. coli

Depositor

James Inglese

Article

van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

Insert

M. orale iPGM bio-6His

Tags

His tag and biotinylation sequence

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pET21a Hp-iPGM-bio

Plasmid

#179615

Purpose

Expresses H. pylori iPGM containing a C-terminal biotinylation site and His tag in E. coli

Depositor

James Inglese

Article

van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

Insert

H. pylori iPGM bio-6His

Tags

His tag and biotinylation sequence

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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HIS_TEV_hsSYX3

Plasmid

#179290

Purpose

E. coli expression plasmid (T7lac promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + TEV protease cleavage site

Depositor

Debra Hansen

Article

Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

Insert

Syx

Tags

His6 + TEV protease cleavage site

Expression

Bacterial

Mutation

residues 393-792 from accession number NP_0010361…

Promoter

T7lac

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pJJGL003

Plasmid

#180607

Purpose

Plasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX+Region3 insertion

Depositor

Jennifer Doudna

Article

Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

Insert

DpbCasX with R3 loop

Use

CRISPR

Tags

10x His and MBP

Expression

Bacterial

Promoter

T7

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pET11-PC(75-228)-H6

Plasmid

#183779

Purpose

Express PC(75-228)-H6 in E. coli. PC(75-228)-H6 was used for GST-CBP pull-down assays.

Depositor

Peter Harte

Article

Tie et al Proc Natl Acad Sci U S A. 2016 Feb 9;113

Insert

Polycomb (Pc Fly)

Tags

6 His Tag at the C-terminus

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pET11-PC(75-390)-H6

Plasmid

#183781

Purpose

Express PC(75-390)-H6 in E. coli. PC(75-390)-H6 was used for GST-CBP pull-down assays

Depositor

Peter Harte

Article

Tie et al Proc Natl Acad Sci U S A. 2016 Feb 9;113

Insert

Polycomb (w Fly)

Tags

6 His tag at the C-terminus

Expression

Bacterial

Mutation

6 His tag at the C-terminus

Promoter

T7

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 7861 - 7880 of 9887 results

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