Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 29,768 results for: REP

Showing: 7861 - 7880 of 29768 results

  1. pAAV-UBC-S1-jRCaMP1a

    Plasmid
    #160728
    Purpose
    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter jRCaMP1a (calcium indicator), Xpress tag, and S1 protein scaffold.
    Depositor
    Edward Boyden
    Article
    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31
    Insert
    S1-jRCaMP1a
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Human ubiquitin C (UBC) promoter
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160728-plasmid-map-sequence-id-315534

    Enlarge

  2. pFB1.HMBP.PrS.CBX8ΔChromo

    Plasmid
    #224708
    Purpose
    Expresses a human CBX8 truncation (deletion of the chromodomain) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag
    Depositor
    Chen Davidovich
    Article
    Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.
    Insert
    CBX8 chromodomain truncation (CBX8 Human)
    Use
    Tags
    HMBP
    Expression
    Insect
    Mutation
    Promoter
    Polyhedrin
    Available since
    Oct. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    224708-plasmid-map-sequence-id-449678

    Enlarge

  8. pLX_TRC311/NLS-Cas13d-P2A-Blast

    Plasmid
    #212196
    Purpose
    CasRx (NLS-RfxCas13d-NLS) with 2A-Blasticidin for RNA targeting. CasRx-2A-Blasticidin is driven by EF1a promoter. EGFP is driven by SV40
    Depositor
    Stacey Edwards
    Article
    Wang et al Mol Cancer. 2024 May 15;23(1):101. doi:
    Insert
    CasRx (NLS-RfxCas13d-NLS)-HA with 2A-Blasticidin
    Use
    CRISPR and Lentiviral
    Tags
    HA, NLS, and blasticidin S deaminase
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α promoter
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    212196-plasmid-map-sequence-id-420116

    Enlarge

  15. pCRI003-pGEM-LS-Bar-PgpdA-dSpCas9-VPR-TtrpC-LS

    Plasmid
    #140196
    Purpose
    Chromosomal integration of PgpdA-dSpCas9-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi vector.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Inserts
    dSpCas9-VPR
    Bar
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
    Use
    CRISPR and Synthetic Biology; Fungal genome integ…
    Tags
    VPR (VP64-p65-Rta) , NLS
    Expression
    Mutation
    Promoter
    PgpdA and PtrpC
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    140196-plasmid-map-sequence-id-309706

    Enlarge

  20. SARS-CoV-2 Nucleocapsid-3xHA-IRES-mcherry

    Plasmid
    #173030
    Purpose
    To express SARS-CoV-2 Nucleocapsid protein tagged with 3xHA and in frame with mcherry (NCP-IRES-mcherry) in mammalian cells
    Depositor
    Mohamed Fareh
    Article
    Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:
    Insert
    SARS-CoV-2 Nucleocapsid phosphoprotein
    Use
    Lentiviral
    Tags
    3xHA and IRES-mcherry
    Expression
    Mammalian
    Mutation
    Promoter
    Gag (truncated)
    Available since
    Sept. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    173030-plasmid-map-sequence-id-341538

    Enlarge

Showing: 7861 - 7880 of 29768 results