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pWS939

Plasmid

#123051

Purpose

Pheromone responsive transcription factor

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Ste12 [3]

Expression

Yeast

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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LI C-D 11GFP

Plasmid

#54431

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

superfolder GFP 11 domain

Use

Cloning vector

Available Since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pCAGGS-rat PKA-RIIbeta

Plasmid

#45529

Depositor

Haining Zhong

Article

Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

Insert

PKA regulatory subunit RII beta

Use

With chicken beta actin promoter and wpre

Expression

Mammalian

Available Since

July 18, 2013

Availability

Academic Institutions and Nonprofits only

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BS.YxiN.Twin1.a1-479.wt

Plasmid

#13527

Depositor

David McKay

Article

Karginov et al J Biol Chem. 2005 Oct 21. 280(42):35499-

Insert

B. subtilis DEAD-box helicase YxiN

Tags

intein-chitin binding domain

Expression

Bacterial

Mutation

none

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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LI A-B Ubi (+ intron) c4

Plasmid

#54408

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

L. japonicus polyubiquitin promoter

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pFH68

Plasmid

#128188

Purpose

Level1 ZmUBIp::SpCas9-NG (human codon optimized):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::SpCas9-NG (human codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pRBBm76

Plasmid

#48117

Purpose

Shuttle vector E. coli/B.meg.

Depositor

Dieter Jahn

Article

Biedendieck et al J Biotechnol. 2007 Dec 1;132(4):426-30.

Insert

promoter sacB - gfp

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pPTK012-3a-Inulinase-αMFΔ

Plasmid

#84971

Purpose

Type 3a, Inulinase followed by αMFΔ - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Inulinase followed by αMFΔ secretion tag

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pPTK004-2-pTPI1

Plasmid

#84963

Purpose

Type 2, pTPI1, Triose phosphate isomerase 1 promoter

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Triose phosphate isomerase 1 promoter

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pFH59

Plasmid

#128185

Purpose

Level1 ZmUBIp::StCas9 (Arabidopsis codon optimized):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::StCas9 (Arabidopsis codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFH81

Plasmid

#128192

Purpose

Level1 ZmUBIp::ScCas9 (wheat codon optimized):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::ScCas9 (wheat codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pExp_TRE:TAL14

Plasmid

#51794

Purpose

Inducible expression of TAL14 as input repressor

Depositor

Ron Weiss

Article

Davidsohn et al ACS Synth Biol. 2014 Nov 17.

Insert

TAL14

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE

Available Since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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pClodANL-FGF2YE

Plasmid

#107872

Purpose

Bacterial expression of FGF2phosphomimic-NanoLuc

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Insert

FGF2-phosphomimic

Use

Luciferase

Tags

NanoLuc

Expression

Bacterial

Mutation

Y82E

Promoter

pro D

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pWS067

Plasmid

#123044

Purpose

_-factor GPCR

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Ste2 [3]

Expression

Yeast

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS053

Plasmid

#123025

Purpose

Pheromone responsive promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pFIG1 [2]

Expression

Yeast

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS060

Plasmid

#123024

Purpose

Pheromone responsive promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pFUS1 [2]

Expression

Yeast

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pANY2

Plasmid

#112196

Purpose

This plasmid is used for efficient cloning of DNA and expression of protein in E. coli.

Depositor

Yingfeng An

Article

Gao et al J Agric Food Chem. 2018 Jun 20;66(24):61

Type

Empty backbone

Tags

His

Expression

Bacterial

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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AP-SEMA3C (chick)

Plasmid

#29449

Depositor

Jonathan Raper

Article

Feiner et al Neuron. 1997 Sep . 19(3):539-45.

Insert

AP-SEMA3C (SEMA3C Chicken)

Tags

AP

Expression

Mammalian

Available Since

July 1, 2011

Availability

Academic Institutions and Nonprofits only
pCRISPaint-myc-PuroR

Plasmid

#80961

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

myc tag

Use

Gene tagging

Promoter

none

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pBS cGli3 (CT#34)

Plasmid

#13953

Depositor

Cliff Tabin

Article

Schweitzer et al Mech Dev. 2000 Nov . 98(1-2):171-4.

Insert

Gli3 (GLI3 Chicken)

Expression

Bacterial

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only