We narrowed to 16,556 results for: nans

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pcDNA3.2 mir-1-1 reporter hsa-mir-122

Plasmid

#46673

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

hsa-mir-122 (MIR122 Human)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Escherichia_coli_CVM_N37067PS

Plasmid

#224403

Purpose

For expression of the type I CBASS system encoded by Escherichia coli CVM N37067PS

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

EcCdnF_AGS-C_SLATT6

Expression

Bacterial

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Escherichia_coli_BIDMC103

Plasmid

#224402

Purpose

For expression of the type I CBASS system encoded by Escherichia coli BIDMC103

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

EcCdnF_AGS-C_SLATT6

Expression

Bacterial

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Escherichia_coli_K71-77

Plasmid

#224398

Purpose

For expression of the type I CBASS system encoded by Escherichia coli K71-77

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

Phospholipase_EcCdnE_DisA-like

Expression

Bacterial

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Escherichia_coli_RU1_BHI6

Plasmid

#224397

Purpose

For expression of the type I CBASS system encoded by Escherichia coli RU1 BHI6

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

Cap15_EcCdnE

Expression

Bacterial

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pWP1_mHAL

Plasmid

#102318

Purpose

expression of mouse cDNA of HAL refractory to the guide in plasmid 4

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

Hal (Hal Mouse)

Use

Lentiviral

Promoter

EF1alpha

Available Since

Feb. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pBSFI-myr-akt-S473A

Plasmid

#49190

Purpose

cDNA of Akt1 with myristyolation signal and point mutation from S to A at position 473 in cloning vector

Depositor

Peter Vogt

Article

Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

Insert

Akt1 (Akt1 Mouse)

Use

Adaptor plasmid

Tags

HA tag and myristolation signal

Mutation

S473A

Promoter

T7

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only
pCRISPaint-T2A-Nluc-3xP3-RFP

Plasmid

#127563

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Nluc-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

Nluc

Use

CRISPR

Expression

Insect

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-MSP1D1deltaH4/2H5

Plasmid

#71715

Purpose

Membrane scaffold protein (MSP), helix 4/2 + helix5 deletion

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Tags

6xHis and TEV site

Expression

Bacterial

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG_huBim_Ex3

Plasmid

#85532

Purpose

Inducible expression of guide RNA (huBim_Ex3) with fluorescent GFP reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

hu Bim

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

H1t

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pHS1929

Plasmid

#205968

Purpose

JAGBLH010000220 Cas8-HNH crRNA expression in pCOLADuet-1

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas8-HNH crRNA

Expression

Bacterial

Promoter

T7

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf40

Plasmid

#12728

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf40

Expression

Mammalian

Available Since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
pmEos2-C1-centrin-1

Plasmid

#72588

Purpose

mEos2 photoconvertible fluorescent protein fused to the N-terminus of centrin-1

Depositor

Michel Bornens

Article

Sillibourne et al Cytoskeleton (Hoboken). 2011 Nov;68(11):

Insert

centrin 1 (CETN1 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DO-ChETA-EYFP-WPRE-pA

Plasmid

#37086

Purpose

Expresses ChETA-EYFP in cells lacking Cre (Cre-Off), for optogenetic excitation

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

ChETA-YFP

Use

AAV and Cre/Lox; Cre-off

Tags

EYFP

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pHS0667 KraIscB-1 non-coding locus in pCOLADuet-1

Plasmid

#176537

Purpose

Non-protein coding locus components from KraIscB-1

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

non-coding locus components from KraIscB-1 locus

Expression

Bacterial

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAD-CMV-STAT5B-DN-CMV-GFP

Plasmid

#83262

Purpose

Adenoviral expression of dominant negative STAT5B with co-expression of GFP

Depositor

Andrew Brooks

Article

Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

Inserts

Stat5b (Stat5b Mouse)
eGFP

Use

Adenoviral

Tags

FLAG

Mutation

dominant negative (Y699F)

Promoter

CMV

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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V5-bArr1

Plasmid

#201491

Purpose

Expression of the beta-arrestin1 containing a N-terminal V5 tag.

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

V5-bArr1 (ARRB1 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-mEos3.1 (mEosFP3.1-A69T)

Plasmid

#99221

Purpose

Bacterial expression of the primed conversion capable protein pr-mEos3.1 (mEos3.1-A69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos3.1

Expression

Bacterial

Mutation

A69T for primed conversion applications

Promoter

T7

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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CMV-hCas9-T2A-mStrawberry

Plasmid

#187224

Purpose

Expresses human Cas9 nuclease and mStrawberry via T2A linker under control of a CMV promoter.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

Cas9

Use

CRISPR

Tags

Inserted T2A-mStrawberry at 3' terminal of M…

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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EYFP-IKB

Plasmid

#111194

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

Insert

IKB-alpha

Tags

EYFP

Expression

Mammalian

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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