We narrowed to 35,499 results for: LAT
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Plasmid#37136DepositorInsertLSSmOrange
Tagshuman beta-actinExpressionMammalianMutationA44V/F84L/W145M/I165D/M167L/G202D compared to mOr…PromoterCMVAvailable SinceJune 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLSSmOrange-alpha-Tubulin
Plasmid#37137DepositorInsertLSSmOrange
Tagshuman alpha-tubulinExpressionMammalianMutationA44V/F84L/W145M/I165D/M167L/G202D compared to mOr…PromoterCMVAvailable SinceJune 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter TCF (1) site mutant pGL3Basic
Plasmid#32733DepositorInsertCCND1 promoter (CCND1 Human)
UseLuciferaseMutation-75 TCF(1) site CTTTGAT deletionPromoter-962 CCND1Available SinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
RSRF-Luc-2mut
Plasmid#31819DepositorInsertMutant MEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)
UseLuciferaseExpressionMammalianMutationtwo CTATATTTAG RSRF sites mutated to CGATATTTCGPromotermutated MEF2-dependent (-307 to -242 of Nur77/NR4…Available SinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
HOXA2 (murine) HIS-tag pET
Plasmid#8557DepositorInsertHOXA2 (Hoxa2 Mouse)
TagsHis and T7ExpressionBacterialMutationcontains 5 additional amino acids from 5' un…Available SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-116:PCNA-mEGFP
Plasmid#170818PurposeHomology arms and mEGFP-linker sequence for N-terminus tagging of human PCNADepositorInsertPCNA Homology Arms with mEGFP-linker (PCNA Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceJune 11, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET30a-His6-ABP-LOXCATmut
Plasmid#139972PurposeA control for His6-ABP-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)DepositorInsertA fusion of lactate oxidase from A. viridans and catalase from E. coli
TagsHis6ExpressionBacterialMutationMutation in H265A and R268A in LOX (used original…PromoterT7Available SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
ssAAV-EF1a-FLuc-WPRE-HgHpA_bac_293
Plasmid#118412PurposeExpresses firefly luciferase under the control of a strong ubiquitous promoter EF1a along with a WPRE cassette. Backbone is compatible with both baculoviral and human production methods of AAV.DepositorInsertFirefly luciferase
UseAAV, Luciferase, and Synthetic BiologyExpressionInsect and MammalianPromoterEF1aAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSD_2xNLS-SpCas9MT3-NLS-3xHA-NLS-dSaCas9-2xNLS
Plasmid#107318PurposeExpresses SpCas9 (R1335K) fused to dSaCas9 in mammalian cellsDepositorInsertSpCas9-SaCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 (R1335K) fused to nuclease dead SaCas9 (D1…PromoterCMV IE94Available SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
Dnase1L3 R206C pIRES-DsRed
Plasmid#98847PurposeExpresses Human Dnase1L3 with activity-reducing HUVS mutation R206C in mammalian cells along with DsRedDepositorInsertDnase1L3 (DNASE1L3 Human)
TagsIRES DsRedExpressionMammalianMutationR206C which greatly reduces nuclease activityPromoterCMVAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-C1-iC++_CA
Plasmid#98168Purposeslow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with cyan light, inactivation max with 590 nm (accelarates closure to ms). Codon optimized for mammalian expression.DepositorInsertSynthetic construct iC++_C128A gene
TagsmCherryExpressionMammalianMutationT59S, E83N, E90Q, E101S, V117R, E123S, C128A, V24…PromoterCMV (+enhancer)Available SinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLXSN-EGFR
Plasmid#65226PurposeExpression of EGFR in mammalian cellsDepositorAvailable SinceOct. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJFRC210-10XUAS-FRT>STOP>FRT-myr::smGFP-OLLAS
Plasmid#63170PurposeGAL4-responsive UAS “flp-On” spaghetti monster GFP-OLLAS reporterDepositorInsert"spaghetti monster GFP-OLLAS"
TagsN-myristoylationExpressionInsectAvailable SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
MLM3743
Plasmid#49962PurposeExpresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene for use as a controlDepositorInsert3x Flag Tet1CDmut HB-5 (HBB Human)
UseTALENTags3x FLAGMutationcatalytic domain of TET1 inactive (H1671Y and D16…PromoterEF1aAvailable SinceDec. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-His-Max-A-PQBP1
Plasmid#25802DepositorAvailable SinceJuly 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
200 bp Axin2 mutant
Plasmid#25704DepositorInsertAxin 2 promoter (AXIN2 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationTCF DNA binding site at bp -108 to -102 mutated …Available SinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
hTRAF2K31RpLPCX-HA-Flag/P876
Plasmid#20231DepositorInsertTNF receptor-associated factor 2 (TRAF2 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationK31R (a92g in the TRAF2 CDS sequence).Available SinceMarch 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
XE257 pCDNA3.1(zeo)-hDsh3-truncDEP
Plasmid#16752DepositorInsertFLAG-hDsh3-trunc before DEP (DVL3 Human)
TagsflagExpressionMammalianMutationtruncated before DEPAvailable SinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
XE255 pCDNA3.1(zeo)-hDsh3-truncPDZ
Plasmid#16754DepositorInsertFLAG-hDsh3-trunc before PDZ (DVL3 Human)
TagsflagExpressionMammalianMutationtruncated before PDZAvailable SinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBV-Luc/Del-7
Plasmid#14968DepositorInsertc-myc promoter (-26/+334) (MYC Human)
UseLuciferaseExpressionMammalianMutation-26/+334 (relative to the P2 transcription initia…Available SinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only