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SAE2-mGarnet

Plasmid

#175240

Purpose

Bacterial expression and purification, combined with SAE1 to form the E1 enzyme heterodimer

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

UBA2 (UBA2 Human)

Expression

Bacterial

Promoter

tac

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pRK7 IKK2/IKKb

Plasmid

#26203

Depositor

Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

I-kappa-B kinase beta (IKBKB Human)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
pU6-Decoy

Plasmid

#80324

Purpose

Encodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondii

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

Decoy sgRNA

Use

CRISPR

Promoter

U6

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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MAC_N_CCDC93

Plasmid

#209687

Purpose

Expresses MAC-tagged CCDC93 in mammalian cells

Depositor

Markku Varjosalo

Article

Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-

Insert

CCDC93 (CCDC93 Human)

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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CFAST-Halotag-ER

Plasmid

#214413

Purpose

ER localization of CFAST fused to Halotag-ER

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

rat cb5 ERM

Tags

CFAST-Halo

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HseIF4A3_I

Plasmid

#146578

Purpose

Mammalian Expression of HseIF4A3

Depositor

Elisa Izaurralde

Article

Palacios et al Nature. 2004 Feb 19. 427(6976):753-7.

Insert

HseIF4A3 (EIF4A3 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-SUV39h1 ts2

Plasmid

#115881

Purpose

SUV39h1 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SUV39h1 shRNA (SUV39H1 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pJM189A

Plasmid

#218135

Purpose

For hybrid circuit activation. Expresses E. coli RNAP omega subunit fused to ZF degron SD0.

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD0

Tags

rpoZ

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HSPA2

Plasmid

#19458

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

HSPA2 (HSPA2 Human)

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-SauriCas9-Puro

Plasmid

#135965

Purpose

Expresses SauriCas9 and puromycin resistance genes, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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pMX-Flag-WIP

Plasmid

#11772

Depositor

Jack Strominger

Article

Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

Insert

WIP (WIPF1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pAPM-D4 miR30-SUV39h1 ts1

Plasmid

#115880

Purpose

SUV39h1 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SUV39h1 shRNA (SUV39H1 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-eCas9-sgMIB1

Plasmid

#140239

Purpose

Lentiviral vector expressing high-specificity eSpCas9(1.1) and a gRNA targeting human MIB1

Depositor

Urs Greber

Article

Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

Insert

sgRNA targeting human MIB1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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Vh-HA-FKBP

Plasmid

#20220

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

FKBP (FKBP1A Human)

Tags

HA and Vh

Expression

Mammalian

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pSUPER IKKbeta

Plasmid

#26209

Depositor

Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

pSUPER IKK beta (IKBKB Human)

Use

RNAi

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pHis2-Arf1 (17-181)-Q71L

Plasmid

#184808

Purpose

Bacterial expression of Arf1 (aa17-181, Q71L mutation)

Depositor

James Hurley

Article

Ren et al Cell. 2013 Feb 14;152(4):755-67. doi: 10

Insert

Arf1 (ARF1 Human)

Tags

His6

Expression

Bacterial

Mutation

aa 17-181 only, Q71L

Promoter

T7

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pOG-Cre

Plasmid

#233265

Purpose

Plasmid expressing Cre recombinase that is used to insert DNA CARGO into RMCE homing locus in Rosa26

Depositor

Mauro Calabrese

Article

Trotman et al Mol Cell. 2025 Mar 20;85(6):1117-1133.e1

Insert

Cre recombinase

Use

Cre/Lox

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pHygromycin (GB0211)

Plasmid

#68204

Purpose

Provides the CDS of the Hygromycin resistance gene as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Hygromycin resistance gene coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only

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F-CDK8L

Plasmid

#24762

Depositor

Joan Conaway, Ronald Conaway

Article

Conaway et al FEBS Lett. 2005 Feb 7. 579(4):904-8.

Insert

F-CDK8L (CDK19 Human)

Tags

FLAG

Expression

Mammalian

Available Since

May 14, 2010

Availability

Academic Institutions and Nonprofits only
pJRH056

Plasmid

#171637

Purpose

Plasmid containing a U6 promoter expressing a spyCas9 tdTom sgRNA298 and CAG promoter expressing mTagBFP2

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-tdTomato
mTagBFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CAG and U6

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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