We narrowed to 10,221 results for: gnas
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Plasmid#69547PurposeDonor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.DepositorInsertsUseMouse TargetingTags2x nuclear localization signal and PVT1-2A "…MutationSilent V205V mutation in myogenin gene to prevent…Available SinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only
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pcDNA-NES-ZapCmR1.1
Plasmid#59014PurposeCytosolic zinc-binding FRET biosensorDepositorInsertNES-ZapCmR1.1 genetically encoded cytosolic Zn(II) sensor
TagsNuclear Exclusion SignalExpressionMammalianMutationSee commentPromoterCMVAvailable SinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDH_ss-SBP-EGFP
Plasmid#172358PurposeReporter to monitoring a streptavidin-binding peptide traffickingDepositorInsertER importing signal sequence
UseLentiviralTagsSBP-EGFPExpressionMammalianPromoterCMVAvailable SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBH31(gfcD)
Plasmid#176199PurposeEncodes pelC signal sequence followed by His6-GfcD. Should secrete to periplasmDepositorInsertgfcD
TagspelC leader-6xHisExpressionBacterialPromoterT7Available SinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBH31(gfcD-10xHis)
Plasmid#176200PurposeEncodes pelC signal sequence followed by His10-GfcD. Should secrete to periplasmDepositorInsertgfcD
TagspelC leader-10xHisExpressionBacterialPromoterT7Available SinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
EC0_2_VP64
Plasmid#163714PurposePlasmid encoding VP64 - SV40 NLS as a Type 4a part to be used in the Dueber YTK system (MoClo kit)DepositorInsertVP64 + SV40 NLS
UseSynthetic Biology; Moclo vector level 0 (type 4a)Available SinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCNL-N1_PTS2
Plasmid#65676PurposeExpresses PTS2-CNL in mammalian cellsDepositorInsertperoxisomal targeting signal 2
UseLuciferaseTagsCNLExpressionMammalianPromoterCMVAvailable SinceJune 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGK415-phlTA2-1E
Plasmid#165970PurposeExpresses phlTA(2-1E) mutantDepositorInsertphlF
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…PromoterpPGK1 mutantAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Split-RESCUE-C
Plasmid#170154PurposeAAV vector carrying the RESCUE-DDC-λN (RESCUE is the evolved ADAR2 variant for C-U editing) with a nuclear export signalDepositorInsertRESCUE-DDC-λN-NES
UseAAVExpressionMammalianPromoterCMVAvailable SinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
E2_pENTR_R4R3_T CTP
Plasmid#171736PurposeptpTALECD vector assembly, Entry clone 2 with attR4 and R3DepositorInsertA terminator of Arabidopsis heat shock protein, RPS5A promoter, and RecA1 plastid targeting signal
UseMultisite gateway entry vectorAvailable SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJEP223-AAV-EFS-GFP-pA
Plasmid#62913PurposeAAV vector backbone designed to express GFP from a EFS promoter. It also contains a poly-Adenylation Signal (pA)DepositorInsertGreen Fluorescent protein (GFP)
UseAAVPromotershort form of Elongation Factor1α (EFS)Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
EPHA7 (3NRU)
Plasmid#28126PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertEPHA7:SDC173-D03:C209131 (EPHA7 Human)
TagsHis and Honeybee melittin Signal sequence (MKFLVN…ExpressionBacterialMutationcontains amino acids residues 32-204.Available SinceFeb. 10, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCoofy62
Plasmid#122014PurposeMammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His6-Smt3Star (SumoStar*) and different C-tag optionsDepositorTypeEmpty backboneTagsHis6-Smt3Star and Multi3: depending on the LP2 pr…ExpressionMammalianAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-(RTRG)6
Plasmid#69849PurposeThe RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeatsDepositorInsert6 RTRG repeats
TagsEGFPExpressionMammalianPromoterCMVAvailable SinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-λN-βLac
Plasmid#162039PurposeExpresion of the fusion protein of the λN domain of the λ phage and ampR gene (encoding the enzyme β-lactamase without the native signal sequence)DepositorInsertλN domain of the λ phage and a β-lactamase fusion protein
Tags6xHis tagExpressionBacterialPromoterT7Available SinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
MTK3a_001
Plasmid#123754PurposeEncodes plasma membrane localization signal from Lyn kinase as a Type 3a part to be used in the MTKDepositorInsertLYN
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
p068 pCS mTRPS1 (M)
Plasmid#11040DepositorInsertTRPS1 mut (Trps1 Mouse)
UseXenopus, mammalian, avian, and zebrafishMutationTwo Cys residues at positions 917 and 920 in the …Available SinceDec. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pUASt-ssBiP-ESCargo*
Plasmid#140165PurposeExpresses a regulatable secretory cargo (without an ER export signal) for Drosophila melanogasterDepositorInsertssBip-DsRed-Express2-FKBP(LV)(C22V)
ExpressionInsectPromoterhs70Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
nucHCB2
Plasmid#98581PurposeNuclear Huntingtin Chromobody 2: "iVHH4" intrabody recognizing amino acids 49–148 of huntingtin, fused to eYFP and nuclear localization signal.DepositorInsertHCB2
TagsNLS and YFPExpressionMammalianPromoterCMVAvailable SinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
MTK4b_001
Plasmid#123840PurposeEncodes Bovine Growth Hormone polyadenylation signal as a Type 4b part to be used in the MTK systemDepositorInsertBgh pA
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only