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pSARSF505

Plasmid

#46832

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

GFPN-NpuDnaE_intein-GFP_C

Tags

GFP_C and H6-GFP_N

Expression

Bacterial

Mutation

stemmer GFP with superfolder mutations S30R and Y…

Promoter

T7

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HER2-DMVF-181-N

Plasmid

#87622

Purpose

N-terminal Dronpa fragment fused to the C-terminus of full-length HER2; for BiFC.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

HER2-DMVF181-N (ERBB2 Human, Synthetic)

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_ERBB2_WT

Plasmid

#82113

Purpose

Gateway Donor vector containing ERBB2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ERBB2 (ERBB2 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fSNAP-Hygromycin

Plasmid

#188914

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fSNAP-Hygromycin

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNATM3.1/V5-His TOPO-PKCi-K274W

Plasmid

#55690

Purpose

expresses dominant negative human PKCi tagged with V5

Depositor

Pedro Salas

Article

Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

Insert

Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)

Tags

V5/His

Expression

Mammalian

Mutation

A120E and K274W

Promoter

CMV

Available Since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only
mEmerald Hc-SRC-N-18

Plasmid

#54118

Purpose

Localization: Membrane/Vesicles, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Hc-SRC (SRC Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pWZL Neo Myr Flag PACSIN1

Plasmid

#20545

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PACSIN1 (PACSIN1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pSpCas9_U6_sgRNA_Mettl3C

Plasmid

#165420

Purpose

sgRNA construct for targeting Mettl3 C-terminus

Depositor

Neil Brockdorff

Article

Wei et al Genome Res. 2021 Aug;31(8):1395-1408. do

Insert

PspCas9 gRNA targeting Mettl3 C-terminus (Mettl3 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_HAUS1_WT

Plasmid

#81790

Purpose

Gateway Donor vector containing HAUS1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HAUS1 (HAUS1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pVITRO1-SS-271

Plasmid

#68772

Purpose

Sensor pair 1 V3

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

C-Terminal Sensor 1 V3
N-Terminal Sensor 1 V3

Tags

NLS, flag, and nls

Expression

Mammalian

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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LHP599 - pACT2-Ub

Plasmid

#32170

Depositor

Linda Hicke

Insert

synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)

Tags

Gal4 AD and HA

Expression

Yeast

Promoter

pADH1

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
ATF4 19: (SL)RLuc.5’ATF4.GFP

Plasmid

#21865

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Stem Loop upstream of ATF4

Available Since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only
ATF4 15: 5’ATF4.uORF1stopm1.GFP

Plasmid

#21862

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Stop codon for uORF1 converted to UCG (see author…

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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NanoBRET BiBRET Vector, HaloTag-KRAS(G12C)_BRAF-NanoLuc

Plasmid

#238566

Purpose

Express HaloTag-KRAS(G12C)_BRAF-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Inserts

KRAS (KRAS Human)
BRAF (BRAF Human)

Tags

HaloTag (R) and NanoLuc (R)

Expression

Mammalian

Mutation

G12C

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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CDK9 gRNA (BRDN0001162576)

Plasmid

#77170

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK9

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK9 (CDK9 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ9713: pHR-PGK-SV40_NLS-dCasMINI-V4-QNZF-c-Myc_NLS-mCherry-WPRE

Plasmid

#203219

Purpose

Expression of dCasMINI-V4-QNZF-mCherry in mammalian cells

Depositor

Stanley Qi

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCasMINI-V4-QNZF-mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-ERBB2 C311S

Plasmid

#40985

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Insert

ERBB2 (ERBB2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

C311S

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
pMGS59(AID-ARF-P2A-Hygromycin)

Plasmid

#138174

Purpose

An AID-ARF-P2A-Hygromycin fusion cassette to tag the protein of interests with auxin-inducible degron and ARF at the c-terminal

Depositor

Michael Guertin

Article

Sathyan et al Curr Protoc Mol Biol. 2020 Sep;132(1):e1

Insert

AID-ARF-P2A-Hygromycin

Tags

AID-ARF clamp degron tag

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pNH11 (pmyo-2::Arch(D95N)::2xMycTag)

Plasmid

#130275

Purpose

Expresses the voltage sensor Arch(D95N) in the pharynx of C. elegans.

Depositor

Alexander Gottschalk

Article

Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

Insert

Arch(D95N)

Tags

2x Myc-tag

Expression

Worm

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-UBC4

Plasmid

#104668

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBC4 (UBC4 Mustard Weed)

Use

Synthetic Biology

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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