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pKM294

Plasmid

#134179

Purpose

Mammalian expression of CENP-C and KNL2

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

mcherry-CENP-C 510-end-KNL2 (CENPC Human)

Tags

mCherry

Expression

Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pFX-β-tubulin-SECFP

Plasmid

#175826

Purpose

Express SECFP with microtubule-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

SECFP with microtuble-targeting sequence

Tags

human tubulin beta 4B class IVb;TUBB4B

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1Xpress-Bcr-HisB

Plasmid

#32508

Depositor

John Groffen, Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

BCR (BCR Human)

Tags

HISB

Expression

Mammalian

Available Since

Jan. 26, 2012

Availability

Academic Institutions and Nonprofits only
pFX-EGFP-KRasCT

Plasmid

#175833

Purpose

Express EGFP with plasma membrane-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EGFP with plasma membrane-targeting sequence

Tags

C-terminal hypervariable region of K-Ras 4B

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pPHR-EYFP-NLS

Plasmid

#103820

Purpose

Soluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-EYFP-NLS (CRY2 Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pJN601

Plasmid

#59790

Purpose

zf1::yfp fusion with floxed unc-119(+) within an intron of yfp. For creating zf1::yfp knock-ins using unc-119 as a marker

Depositor

Jeremy Nance

Article

Armenti et al Development. 2014 Dec;141(23):4640-7. do

Inserts

ZF1 domain from pie-1
N-terminal half of yfp with introns
intron containing floxed unc-119 gene in reverse orientation
C-terminal half of yfp with introns

Expression

Worm

Available Since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pFX-β-tubulin-Sirius

Plasmid

#175825

Purpose

Express Sirius with microtubule-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with microtuble-targeting sequence

Tags

human tubulin beta 4B class IVb;TUBB4B

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pET15b-ITPA

Plasmid

#60824

Purpose

to purify human ITPA from bacteria

Depositor

Youri Pavlov

Article

Porta et al Acta Crystallogr Sect F Struct Biol Crys

Insert

ITPA (ITPA Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_190-303

Plasmid

#118822

Purpose

Bacterial expression of hnRNPA2 truncation (residues 190 to 302).

Depositor

Nicolas Fawzi

Article

Amaya et al J Biol Chem. 2018 Dec 21;293(51):19522-1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

His tag and TEV

Expression

Bacterial

Mutation

contains amino acids 190-302

Available Since

April 25, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-MBP-(CBX1)3-eReader

Plasmid

#135090

Purpose

Bacterial expression of engineered CBX1 fusions with enhanced binding to H3K9me

Depositor

Marcey Waters

Article

Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

Insert

CBX1 chromobox protein 1, engineered x3

Tags

6xhis-tag and MBP

Expression

Bacterial

Mutation

lysine 43 mutated to alanine; aspartic acid 59 mu…

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGEM-7Zf-TYR

Plasmid

#32786

Depositor

Ruth Halaban

Article

Ujvari et al J Biol Chem. 2001 Feb 23;276(8):5924-31.

Insert

Tyrosinase (TYR Human)

Use

In vitro transcription

Mutation

I181M

Promoter

T7

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pRLCon1-3180

Plasmid

#31445

Depositor

Gerhart Ryffel

Article

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR (long) (HNF4A Human)

Use

Luciferase

Expression

Mammalian

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pFX-Lifeact-iRFP

Plasmid

#175823

Purpose

Express iRFP with actin-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

iRFP with actin-targeting sequence

Tags

Lifeact (N-terminal 17 amino acids from S. Cerevi…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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EYFP-Abr

Plasmid

#36414

Depositor

John Groffen, Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

active BCR-related (ABR Human)

Tags

EYFP

Expression

Mammalian

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only
pmyc-GFP-TNRC6A-NLS/NES-mut

Plasmid

#42002

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

EGFP and Myc

Expression

Mammalian

Mutation

R930A, R931A, R932A, R934A, F951A, F955A, I958A, …

Promoter

CMV

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only

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pCaSpeR4 cyto Grx1-roGFP2

Plasmid

#64999

Purpose

Expression of cytosolic Grx1-roGFP2 under the alphaTub84B promoter

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Glutaredoxin-1 (GLRX Human)

Tags

roGFP2

Expression

Insect

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pFX-Lifeact-Venus

Plasmid

#174478

Purpose

Express Venus with actin-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Venus with actin-targeting sequence

Tags

Lifeact (N-terminal 17 amino acids from S. Cerevi…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-Venus-KRasCT

Plasmid

#175834

Purpose

Express Venus with plasma membrane-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Venus with plasma membrane-targeting sequence

Tags

C-terminal hypervariable region of K-Ras 4B

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pJS278

Plasmid

#134139

Purpose

Bacterial expression of human Ska1 microtubule binding domain (MTBD)

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-Precission-human Ska1 MTBD (SKA1 Human)

Expression

Bacterial

Mutation

aa 132-255, R155A

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pYZ_NAND2_L17_S19_S11

Plasmid

#132730

Purpose

T7 RNAP-driven expression of a 2-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 19 and 11

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_NAND2_L17_S19_S11

Expression

Bacterial

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pISH-Gnas

Plasmid

#105483

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2014 Jan;58:1-10. doi

Insert

Gnas (Gnas Mouse)

Use

To ganerate riboprobe

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCRII-Sema4d-ISH-2kb

Plasmid

#68032

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Semaphorin-4D (Sema4d Mouse)

Use

Mrna in situ hybridization probe

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pCRII-Plxnb1-ISH-2kb

Plasmid

#68035

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Plexin-B1 (Plxnb1 Mouse)

Use

Mrna in situ hybridization probe

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pRLCon1-1746

Plasmid

#31446

Depositor

Gerhart Ryffel

Article

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR (short) (HNF4A Human)

Use

Luciferase

Expression

Mammalian

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1Xpress-BcrR1090A-HisB

Plasmid

#32507

Depositor

John Groffen, Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

BCR (BCR )

Tags

HISB

Expression

Mammalian

Mutation

R 1090 to A

Available Since

Jan. 26, 2012

Availability

Academic Institutions and Nonprofits only
Bcr DH domain pGEX

Plasmid

#38179

Depositor

Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

BCR DH domain (BCR Human)

Tags

GST

Expression

Bacterial

Mutation

BCR DH domain

Available Since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only
NG0888 pCI-TPI-PTC48-HBB

Plasmid

#65802

Purpose

NMD reporter mRNA

Depositor

Niels Gehring

Article

Clerici et al Nucleic Acids Res. 2014 Feb;42(4):2673-8

Insert

TPI (TPI1 Human)

Tags

HBB (beta-globin 3'UTR for probe binding)

Expression

Mammalian

Mutation

premature stop codon at 48

Promoter

CMV

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pJ02B2Gm:Rm_AF

Plasmid

#66061

Purpose

MoClo Device: FACS Standard Color Controls - High GFP (first unit) & RFP (second unit) expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E:J23102:B:BCD2:C:E1010m:D:B0015:F]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

FACS Controls

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pJ02B2Rm:Gm_AF

Plasmid

#66062

Purpose

MoClo Device: FACS Standard Color Controls - High RFP (first unit) & GFP (second unit) expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E1010m:D:B0015:E:J23102:B:BCD2:C:E0040m:D:B0015:F]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

FACS Controls

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pBMN GBRP

Plasmid

#89293

Purpose

For expression of the mammalian Atg8 protein GABARAP without a tag

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

GABARAP (GABARAP Human)

Use

Retroviral

Expression

Mammalian

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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