We narrowed to 108,707 results for: LAS

1. Human PNP his-tagged

   Plasmid

   #64076

   Purpose

   Expression of human Purine Nucleoside Phosphorylase

   Depositor

   Vern Schramm

   Article

   Lewandowicz et al J Biol Chem. 2003 Aug 22;278(34):31465-8

   Insert

   Purine Nucleoside Phosphorylase (PNP Human)
   

   Use

   Tags

   Histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pET28a-Ec.coaBC (pCLK1210)

   Plasmid

   #50448

   Purpose

   Heterologous expression of E. coli CoA biosynthetic enzyme, PPCS_PPCDC - phosphopantothenoylcysteine synthetase_phosphopantothenoylcysteine decarboxylase

   Depositor

   Erick Strauss, Tadhg Begley

   Article

   Strauss et al J Biol Chem. 2001 Apr 27;276(17):13513-6

   Insert

   PPCS PPCDC
   

   Use

   Tags

   6XHIS tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Ii-OVA.II

   Plasmid

   #64597

   Purpose

   Expression of invariant chain fused to chicken ovalbumin 315-353 amino acids as model antigen for study of MHC class II presentation

   Depositor

   Sandra Diebold, Martin Zenke

   Article

   Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

   Insert

   Ovalbumin
   

   Use

   Tags

   Invariant chain

   Expression

   Mammalian

   Mutation

   Only amino acids 315-353 (class II epitopes)

   Promoter

   CMV

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmiRFP713-N1

   Plasmid

   #136559

   Purpose

   A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP713

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   miRFP713
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLIC-SM-HA

   Plasmid

   #111188

   Purpose

   High affinity HA Tagging of weakly expressed genes (optimized for Toxoplasma gondii)

   Depositor

   Silvia Moreno

   Article

   Hortua Triana et al J Eukaryot Microbiol. 2018 Apr 19. doi:

   Type

   Empty backbone

   Use

   Toxoplasma gondii expression

   Tags

   Spaghetti Monster HA Tag

   Expression

   Mutation

   Promoter

   Endogenous

   Available since

   Aug. 2, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pLIC-SM-Myc

   Plasmid

   #111189

   Purpose

   High affinity Myc tagging of weakly expressed genes (optimized for Toxoplasma gondii)

   Depositor

   Silvia Moreno

   Article

   Hortua Triana et al J Eukaryot Microbiol. 2018 Apr 19. doi:

   Type

   Empty backbone

   Use

   Toxoplasma gondii expression

   Tags

   Spaghetti Monster Myc Tag

   Expression

   Mutation

   Promoter

   Endogenous

   Available since

   Aug. 2, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pFREE_Amp

   Plasmid

   #92051

   Purpose

   Allows inducible expression of gRNAs and Cas9 for plasmid curing.

   Depositor

   Morten Norholm

   Article

   Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

   Inserts

   Cas9
   gRNA array
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   pRhamBAD and ptet

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDECKO_mCherry

   Plasmid

   #78534

   Purpose

   Backbone to clone single or paired sgRNAs

   Depositor

   Rory Johnson, Roderic Guigo

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (for expressing sgRNA)

   Available since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. phMYT1L-N106

   Plasmid

   #31782

   Depositor

   Jerry Crabtree

   Article

   Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

   Insert

   myelin transcription factor 1-like (MYT1L Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p181 pK7-HDAC1 (GFP)

    Plasmid

    #11054

    Depositor

    Ramesh Shivdasani

    Article

    Tou et al Mol Cell Biol. 2004 Apr . 24(8):3132-9.

    Insert

    HDAC1 (Hdac1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSW002-Pc-E2-Crimson

    Plasmid

    #111252

    Purpose

    Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with E2-Crimson

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    E2-Crimson
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pc

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFREE_Cm

    Plasmid

    #92052

    Purpose

    Allows inducible expression of gRNAs and Cas9 for plasmid curing.

    Depositor

    Morten Norholm

    Article

    Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

    Inserts

    Cas9
    gRNA array
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    pRhamBAD and ptet

    Available since

    Aug. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLVU/RED

    Plasmid

    #24178

    Depositor

    Lars Ittner

    Article

    Krupka et al Plasmid. 2010 May;63(3):155-60. doi: 10.

    Insert

    Chloramphenicol resistance gene (CmR) - ccdB gene
    

    Use

    Lentiviral; Gateway

    Tags

    DsRED

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. Mouse 3' Hp1a AID GFP PuroR

    Plasmid

    #127897

    Purpose

    HDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Mouse Hp1a Gene

    Depositor

    Mark Groudine

    Insert

    Hp1a (Cbx5 Mouse, Mustard Weed)
    

    Use

    Donor plasmid

    Tags

    GFP, AID, PuroR

    Expression

    Mutation

    Inserting GFP AID 2A Puro into mouse 3' Hp1a

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-Mycn

    Plasmid

    #60667

    Purpose

    PiggyBac expression vector encoding murine Mycn downstream of a doxycycline inducible promoter

    Depositor

    James Thomson

    Article

    Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

    Insert

    Mycn (Mycn Mouse)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pT7-EGFP-C1-HsNot3

    Plasmid

    #37372

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

    Insert

    HsNot3 (CNOT3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMXs-Oct3/4-IP

    Plasmid

    #15918

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    POU domain, class 5, transcription factor 1 (Pou5f1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNES-iRFP-C1

    Plasmid

    #116863

    Purpose

    cytosolic iRFP

    Depositor

    Gerry Hammond

    Article

    Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

    Insert

    iRFP713
    

    Use

    Tags

    X.leavis map2k1.L(32-44)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pT7-EGFP-C1-HsNot7

    Plasmid

    #37325

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

    Insert

    HsNot7 (CNOT7 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT7-EGFP-C1-HsNot6

    Plasmid

    #37368

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

    Insert

    HsNot6 (CNOT6 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge