Addgene

We narrowed to 21,948 results for: ESC

Showing: 121 - 160 of 21948 results

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pBluescript-mouse eIF2C2

Plasmid

#50354

Purpose

Mouse eIF2C2 cDNA clone

Depositor

Kumiko Ui-Tei

Article

Doi et al Curr Biol. 2003 Jan 8;13(1):41-6.

Insert

eIF2C2 (Ago2 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pESC-LEU-HsAIDSc

Plasmid

#60810

Purpose

to express human AID recoded for yeast codon usage

Depositor

Thomas Kunkel, Youri Pavlov

Article

Mayorov et al BMC Immunol. 2005 Jun 10;6:10.

Insert

AID (AICDA Human)

Use

Tags

Myc

Expression

Yeast

Mutation

codon optimized for yeast

Promoter

GAL1

Available since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
NCVB-ssGrl1-ESCargo*

Plasmid

#140167

Purpose

Expresses a regulatable secretory cargo (without an ER export signal) for Tetrahymena thermophila

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

ssGrl1-DsRed-Express2-FKBP(LV)(C22V)

Use

Other

Tags

Expression

Mutation

Promoter

MTT1

Available since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBluescript-mouse Dicer

Plasmid

#50357

Purpose

Mouse Dicer cDNA clone

Depositor

Kumiko Ui-Tei

Article

Doi et al Curr Biol. 2003 Jan 8;13(1):41-6.

Insert

Dicer1 (Dicer1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T3

Available since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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YIplac204-pOst1-ESCargo

Plasmid

#115420

Purpose

Expresses a regulatable secretory cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

pOst1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Use

Tags

Expression

Yeast

Mutation

Promoter

TPI1

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pESC-LEU-SopB

Plasmid

#183670

Purpose

Inducible Salmonella Typhimurium SopB for expression in yeast

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBS B2rescue A70D

Plasmid

#78658

Purpose

Generates B2 inserts suitable for rescue

Depositor

Astrid Roy-Engel

Article

Wagstaff et al PLoS Genet. 2012;8(8):e1002842. doi: 10.

Insert

B2rescue cassette

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pBluescript mouse ptc

Plasmid

#58699

Purpose

full length mouse ptc cDNA

Depositor

Matthew Scott

Insert

ptc (Ptch1 Mouse)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

T7

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only
pBluescript alpha-SSeCKS

Plasmid

#61295

Purpose

contains rat alpha SSeCKS cDNA

Depositor

Irwin Gelman

Insert

alpha SSeCKS (Akap12 Rat)

Use

Tags

Expression

Bacterial

Mutation

cDNA with 1kb of 3' UTR and 150bp of 5'…

Promoter

T7

Available since

Dec. 9, 2014

Availability

Academic Institutions and Nonprofits only
YIplac204-pOst1-ESCargo*

Plasmid

#115427

Purpose

Expresses a regulatable secretory cargo (lacking an ER export signal) for yeast at the TRP1 locus

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

pOst1-DsRed-Express2-FKBP(LV)(C22V)

Use

Tags

Expression

Yeast

Mutation

Promoter

TPI1

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe Smad4J Rescue

Plasmid

#19153

Depositor

Joan Massague

Article

Padua et al Cell. 2008 Apr 4. 133(1):66-77.

Insert

SMAD family member 4 (SMAD4 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

G to A at bp 1397 T to C at bp 1398

Promoter

Available since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only
Flag-TRIM24 shRescue

Plasmid

#28139

Depositor

Michelle Barton

Article

Allton et al Proc Natl Acad Sci U S A. 2009 Jul 14. 1

Insert

TRIM24 (TRIM24 Human)

Use

Tags

FLAGX2

Expression

Mammalian

Mutation

A489V; aa 308 and 309 mutated for shRNA rescue

Promoter

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
YIplac128-pOst1-ESCargo

Plasmid

#115421

Purpose

Expresses a regulatable secretory cargo for yeast at the LEU2 locus

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

pOst1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Use

Tags

Expression

Yeast

Mutation

Promoter

TPI1

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pBluescript-mouse eIF2C4

Plasmid

#50356

Purpose

Mouse eIF2C4 cDNA clone

Depositor

Kumiko Ui-Tei

Article

Doi et al Curr Biol. 2003 Jan 8;13(1):41-6.

Insert

eIF2C4 (Ago4 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-THAP11-rescue

Plasmid

#37001

Depositor

Debu Chakravarti

Article

Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

Insert

THAP11 (THAP11 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Mutations were generated at codons 273-275 conver…

Promoter

Available since

May 21, 2012

Availability

Academic Institutions and Nonprofits only
pBluescript-mouse eIF2C1

Plasmid

#50353

Purpose

Mouse eIF2C1 cDNA clone

Depositor

Kumiko Ui-Tei

Article

Doi et al Curr Biol. 2003 Jan 8;13(1):41-6.

Insert

eIF2C1 (Eif2c1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T3

Available since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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HOXA10 (human) bluescript

Plasmid

#8617

Depositor

Corey Largman

Article

Lowney et al Nucleic Acids Res 1991 Jun 25;19(12):344

Insert

HOXA10 (HOXA10 Human)

Use

Tags

Expression

Bacterial

Mutation

improved Ribosomal binding site (NcoI site) at AT…

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pUASt-ssBiP-ESCargo

Plasmid

#140166

Purpose

Expresses a regulatable secretory cargo for Drosophila melanogaster

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

ssBip-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Use

Tags

Expression

Insect

Mutation

Promoter

hs70

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pUASt-ssBiP-ESCargo*

Plasmid

#140165

Purpose

Expresses a regulatable secretory cargo (without an ER export signal) for Drosophila melanogaster

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

ssBip-DsRed-Express2-FKBP(LV)(C22V)

Use

Tags

Expression

Insect

Mutation

Promoter

hs70

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Rescue Septin 11 mRNA

Plasmid

#122025

Purpose

Rescue mRNA for Septin 11 Knock Down with sh2 shRNA. Splice variant II (EU711415) with 5 silent mutations at SEPT11 sh2 shRNA target site.

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Use

Tags

Expression

Mammalian

Mutation

Silent mutations are in 5 consecutive codons (CUG…

Promoter

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pESC-LEU2-RAD30cmyc

Plasmid

#60821

Purpose

to overexpress yeast polymerase eta in yeast

Depositor

Thomas Kunkel, Youri Pavlov

Article

Pavlov et al Mutat Res. 2001 Jul 1;478(1-2):129-39.

Insert

RAD30 (RAD30 Budding Yeast)

Use

Tags

c-myc

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pBluescript-KS(+) CREST (C90)

Plasmid

#22962

Depositor

Anirvan Ghosh

Insert

CREST (Ss18l1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2010

Availability

Academic Institutions and Nonprofits only
HOXB5 (human) pBluescript

Plasmid

#8531

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB5 (HOXB5 Human)

Use

Tags

Expression

Bacterial

Mutation

makes protein in vitro transcription/translation

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
human EB1 in pBluescript II

Plasmid

#39324

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

T7

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pBluescript-KS-Nrg3-ISH

Plasmid

#87669

Purpose

In situ probe for mouse Nrg3

Depositor

Oscar Marin

Article

Bartolini et al Cell Rep. 2017 Jan 31;18(5):1157-1170. d

Insert

Nrg3 (Nrg3 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7/M13

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iEscSnFR_PM

Plasmid

#182813

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_PM

Use

Tags

Expression

Mammalian

Mutation

Promoter

hCMV

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV(MinDis)-iEscSnFR_cyto

Plasmid

#182814

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_cyto

Use

Tags

Expression

Mammalian

Mutation

Promoter

hCMV

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV9-hSyn-iEscSnFR_ER

Plasmid

#182818

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_ER

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV9-hSyn-iEscSnFR_PM

Plasmid

#182819

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_PM

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV9-hSyn-iEscSnFR_cyto

Plasmid

#182820

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_cyto

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV(MinDis)-iEscSnFR_ER

Plasmid

#182812

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_ER

Use

Tags

Expression

Mammalian

Mutation

Promoter

hCMV

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pESC-LEU-IpgD

Plasmid

#183672

Purpose

Inducible Shigella flexneri IpgD for expression in yeast

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

IpgD

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
564aa ELL2 bluescript

Plasmid

#127267

Purpose

For in vitro translation of human ELL2 lacking COOH region

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Tags

Expression

Mutation

Addgene Plasmid 127256--SphI site filled in, then…

Promoter

T7

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
ESCO2 C10.2 gRNA

Plasmid

#90681

Purpose

3rd generation lentiviral gRNA plasmid targeting human ESCO2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ESCO2 (Guide Designation C10.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
ESCO2 C9.2 gRNA

Plasmid

#90680

Purpose

3rd generation lentiviral gRNA plasmid targeting human ESCO2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ESCO2 (Guide Designation C9.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBluescript SK-rPAI-153

Plasmid

#45389

Depositor

Thomas Gelehrter

Article

Zeheb et al Gene. 1988 Dec 20;73(2):459-68.

Insert

Plasminogen activator inhibitor type 1 (Serpine1 Rat)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
pET28b_H6-Smt3-AesC-GFP

Plasmid

#234486

Purpose

Superfolder GFP C-terminally fused to His-Smt3-tagged AesC split intein

Depositor

Henning Mootz

Article

Humberg et al Nat Commun. 2025 Mar 19;16(1):2723. doi:

Insert

C-terminal Fragment of the Aes123 PolB1 Split Intein

Use

Tags

Hexahistidine Tag, Superfolder Green Fluorescent …

Expression

Bacterial

Mutation

Promoter

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
His6-TEV-Venus-pBluescript

Plasmid

#166756

Purpose

For purification of Venus fluorophore

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

Venus

Use

Tags

6x Histidine tag, and TEV protease site

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBluescript-IC3-FLAG-Tev-ZZ

Plasmid

#170311

Purpose

Plasmid for endogenous C-terminal tagging of the Tetrahymena thermophila Outer Dynein Arm intermediate chain 3 with FLAG and ZZ tags.

Depositor

Andrew Carter

Article

Mali et al Science. 2021 Feb 26;371(6532):910-916.

Insert

Outer Dynein Arm Intermediate Chain 3

Use

Tags

FLAG, ZZ

Expression

Mutation

Promoter

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
His6-TEV-mCerulean3-pBluescript

Plasmid

#166757

Purpose

For purification of mCerulean3 fluorophore

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

mCerulean3

Use

Tags

6x Histidine tag, and TEV protease site

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 121 - 160 of 21948 results

Results per page:

Sort results by: