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Showing: 151 - 200 of 8010 results

  1. ND4 alphaDdCBE T1

    Plasmid
    #221984
    Purpose
    Expresses mitochondrial cytosine base editor arm in mammalian cells
    Depositor
    Stephen Ekker
    Article
    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089
    Insert
    COX8A MTS-3xFLAG-TALE NT-alphaN-ND4 TALE T1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    TALE: Q231R, W232G, S233A
    Promoter
    CMV
    Available since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    221984-plasmid-map-sequence-id-438483

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  2. ND4 alphaDdCBE T2

    Plasmid
    #221985
    Purpose
    Expresses mitochondrial cytosine base editor arm in mammalian cells
    Depositor
    Stephen Ekker
    Article
    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089
    Insert
    COX8A MTS-3xFLAG-TALE NT-alphaN-ND4 TALE T2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    TALE: Q231R, W232G, S233A
    Promoter
    CMV
    Available since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    221985-plasmid-map-sequence-id-438489

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  16. pcU6.3_gRNA_c1

    Plasmid
    #221381
    Purpose
    Chicken-specific U6 sgRNA expression mini-vector with 10x capture sequence 1
    Depositor
    James Briscoe, Tatjana Sauka-Spengler
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    G. gallus U6.3
    Available since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    221381-plasmid-map-sequence-id-454411

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  18. pFLAG-6a-Ataxin3Q80

    Plasmid
    #22129
    Depositor
    Henry Paulson
    Insert
    Ataxin 3 (ATXN3 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 21, 2009
    Availability
    Academic Institutions and Nonprofits only
    22129-plasmid-map-sequence-id-408112

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  20. PSMD3_miniTurbo_Nterm

    Plasmid
    #221527
    Purpose
    Flag-tagged biotinylation enzyme for co-expression of Psmd3
    Depositor
    Alessandro Ori
    Insert
    PSMD3 (PSMD3 Human)
    Use
    Tags
    miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    221527-plasmid-map-sequence-id-439439

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  21. pQFmcs-2H12.D11-scarREF

    Plasmid
    #221184
    Purpose
    cdGreen2 expressed from a cumate-inducible promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11240)
    Depositor
    Urs Jenal
    Article
    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:
    Inserts
    cdGreen2
    mScarlet-I
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N-terminus changed: starts with MSKKYGEAVIKE ...
    Promoter
    None (same as cdGreen2) and PQ5 (cumate-inducible)
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    221184-plasmid-map-sequence-id-437178

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  28. pUbi_dCas9-BFP-KRAB-gRNA

    Plasmid
    #221503
    Purpose
    CRISPRi machinery fish codon optimized expressing plasmid
    Depositor
    Alessandro Ori
    Insert
    dCas9-tagBFP-KRAB
    Use
    Fish expression
    Tags
    Expression
    Mutation
    D10A and H839A point mutations
    Promoter
    Zebrafish Ubiquitin promoter
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    221503-plasmid-map-sequence-id-439415

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  30. pTipi2.1_Anti-Biotin [IPI-M33] Mouse/Rabbit HC

    Plasmid
    #221349
    Purpose
    Mammalian expression of Anti-Biotin [IPI-M33] Mouse/Rabbit HC; to be used with Anti-Biotin [IPI-M33] light chain (Plasmid 221350) to make the antibody.
    Depositor
    Institute for Protein Innovation (IPI)
    Insert
    Anti-Biotin [IPI-M33] Mouse/Rabbit HC
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221349-plasmid-map-sequence-id-436706

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  31. pTipi2.1_Anti-Biotin [IPI-M33] Mouse/Rabbit LC kappa

    Plasmid
    #221350
    Purpose
    Mammalian expression of Anti-Biotin [IPI-M33] Mouse/Rabbit LC kappa; to be used with Anti-Biotin [IPI-M33] heavy chain (Plasmid 221349) to make the antibody.
    Depositor
    Institute for Protein Innovation (IPI)
    Insert
    Anti-Biotin [IPI-M33] Mouse/Rabbit LC kappa
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221350-plasmid-map-sequence-id-436707

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  33. pLenti X1 Zeo/pTER shMDC1 (673-1)

    Plasmid
    #22193
    Depositor
    Eric Campeau
    Insert
    sh Mediator of DNA damage checkpoint 1
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 18, 2009
    Availability
    Academic Institutions and Nonprofits only
    22193-plasmid-map-sequence-id-10169

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  34. SMN2E7-Fluc-Nluc-reporter

    Plasmid
    #221005
    Purpose
    Transiently express SMN2 dual luciferase reporter. Inclusion of E7 produces in-frame stop codon and Firefly luciferase only, while skipping produces both Firefly and Nanoluc. CMV promoter.
    Depositor
    Benjamin Blencowe, Mikko Taipale
    Article
    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e
    Insert
    Fluc-SMN2E7-STOP-Nluc
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    221005-plasmid-map-sequence-id-437012

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  45. pBR HPV31 9E

    Plasmid
    #221009
    Purpose
    Human papillomavirus 31 isolate 9E, complete genome cloned in pBR322
    Depositor
    Alison McBride
    Insert
    Human papillomavirus type 31 genome 9E isolate
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    221009-plasmid-map-sequence-id-436075

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  50. PSMA4_GFP_Cterm

    Plasmid
    #221514
    Purpose
    Fluorescently tagged Psma4-GFP
    Depositor
    Alessandro Ori
    Insert
    PSMA4 (PSMA4 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    221514-plasmid-map-sequence-id-439377

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Showing: 151 - 200 of 8010 results