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We narrowed to 209 results for: LOV2

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Showing: 61 - 80 of 209 results

  1. PA-DAD-dark

    Plasmid
    #53096
    Purpose
    Expresses dark mutant PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light with LOV2 in the closed conformation
    Depositor
    Klaus Hahn, Ronen Zaidel-Bar
    Publication
    Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LOV(dark)-6aa-DAD
    Tags
    6x His and mVenus
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    L47F and C49S in Venus; C450M mutation in LOV2
    Available since
    Aug. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    53096-plasmid-map-sequence-id-119521

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  2. CMV-CASANOVA

    Plasmid
    #113035
    Purpose
    CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9
    Depositor
    Dominik Niopek
    Publication
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    113035-plasmid-map-sequence-id-224728

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  4. pCMV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

    Plasmid
    #92390
    Purpose
    expresses Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA component in mammalian cells
    Depositor
    Hyungbae Kwon
    Publication
    Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    92390-plasmid-map-sequence-id-181393

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  6. pBabe-PI(WT)-Src(YF)-mCherry

    Plasmid
    #87357
    Purpose
    PI(WT): Photo-inhibitable/blue light sensitive; YF: Y535F const. active mutation
    Depositor
    Klaus Hahn
    Publication
    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    LOV2
    Src isoform 2 (Src Mouse)
    Tags
    mCherry
    Expression
    Bacterial and Mammalian
    Mutation
    Y535F
    Promoter
    CMV
    Available since
    April 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    87357-plasmid-map-sequence-id-178649

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  10. PA-DAD-wt

    Plasmid
    #53095
    Purpose
    Expresses wild type PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light.
    Depositor
    Klaus Hahn, Ronen Zaidel-Bar
    Publication
    Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LOV(wt)-6aa-DAD
    Tags
    6x His and mVenus
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    L47F and C49S in Venus
    Available since
    Aug. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    53095-plasmid-map-sequence-id-119520

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  14. pEB1N-mScarlet-LZ-LOV2

    Plasmid
    #197419
    Purpose
    Mammalian expression of the mScarlet-tagged N-terminal half of photo-inactivated π-EB1
    Depositor
    Torsten Wittmann
    Publication
    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MAPRE1 (MAPRE1 Human)
    Tags
    A. sativa phototropin 1 LOV2 domain, GCN4 leucine…
    Expression
    Mammalian
    Mutation
    EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…
    Available since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    197419-plasmid-map-sequence-id-388076

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  15. pTriEx-PA-Rac1-C450A

    Plasmid
    #22025
    Purpose
    Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)
    Depositor
    Klaus Hahn
    Publication
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
    Tags
    6X His
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    LOV domain contains light-insensitive mutation C4…
    Promoter
    CMV, p10
    Available since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles

  16. LITE1.0_pAAV_hSyn_CRY2PHR-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

    Plasmid
    #47454
    Purpose
    epiLITE / LITE1.0 CRY2PHR fused to SID4X transcriptional repressor effector domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression.
    Depositor
    Feng Zhang
    Publication
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    CRY2PHR-NLS-SID4X
    Use
    AAV
    Tags
    2A_phiLOV2.1
    Expression
    Mammalian
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available since
    Oct. 15, 2013
    Availability
    Academic Institutions and Nonprofits only
    47454-plasmid-map-sequence-id-69624

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  18. pAAV_hSyn_TALEBB(HD)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

    Plasmid
    #47450
    Purpose
    AAV TALE synthesis backbone with HD half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression.
    Depositor
    Feng Zhang
    Publication
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    TALE BB(N136_0.5HD_C63)-SID4X
    Use
    AAV; Tale
    Tags
    2A_phiLOV2.1
    Expression
    Mammalian
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available since
    Oct. 15, 2013
    Availability
    Academic Institutions and Nonprofits only
    47450-plasmid-map-sequence-id-69442

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Showing: 61 - 80 of 209 results