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Plasmid#154248Purposeexpresses ancestral New World monkey retroCHMP3 in mammalian cellsDepositorInsertreconstructed ancestral sequence of New World monkey retroCHMP3
UseTagsHAExpressionMammalianMutationPromoterEF1-alphaAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF1-puro/hpb1
Plasmid#59976Purposeintegrin b1 short headpiece expression (beta-I, hybrid, PSI and I-EFG1 domains) expressionDepositorInserthuman integrin b1 headpiece (ITGB1 Human)
UseTagsBase coiled-coil and His-tagExpressionMammalianMutationPromoterEF1a promoterAvailable sinceSept. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-CRY2PHR-SID4X
Plasmid#63663PurposeAAV expression of CRYS2PHR-SID4XDepositorInsertSID4X-CYR2PHR
UseAAVTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-mXRCC1pd-eGFP
Plasmid#206035PurposeMammalian expression of PAR-deficient mXRCC1pd coupled to eGFP under the control of a EF1a promoterDepositorInsertmXRCC1pd
UseTagseGFPExpressionMammalianMutationS105A, S186A, K188A, S195A, S221A, S222A,S238A, S…PromoterEf1aAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
vYM004_pNeo_phiC31_nTRE_pEF1s(orig)_citrine
Plasmid#197677Purpose"pEF1s(orig)" reporter plasmidDepositorInsertpEF1s-H2B-Citrine
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF1α -NWM-Node 1
Plasmid#154253Purposeexpresses reconstructed New World monkey retroCHMP3 Node 1 in mammalian cellsDepositorInsertreconstructed Node 1 of New World monkey retroCHMP3
UseTagsHAExpressionMammalianMutationPromoterEF1-alphaAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF1α -NWM-Node 2
Plasmid#154254Purposeexpresses reconstructed New World monkey retroCHMP3 Node 2 in mammalian cellsDepositorInsertreconstructed Node 2 of New World monkey retroCHMP3
UseTagsHAExpressionMammalianMutationPromoterEF1-alphaAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF1α -NWM-Node 3
Plasmid#154255Purposeexpresses reconstructed New World monkey retroCHMP3 Node 3 in mammalian cellsDepositorInsertreconstructed Node 3 of New World monkey retroCHMP3
UseTagsHAExpressionMammalianMutationPromoterEF1-alphaAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-PEmax-P2A-GFP
Plasmid#202000PurposeBased on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.DepositorInsertPEmax-P2A-GFP
UseCRISPR and Synthetic BiologyTagsGFPExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pEF1a-EBFP-tTAn
Plasmid#172122PurposeConstitutive expression of EBFP and rtTA-InteinNDepositorInsertEBFP, tTAn-InteinN
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRRLNeo-pEF1a-p53ash-mCerulean
Plasmid#69579PurposeExpresses p53 tagged with mCeruleanDepositorInsertp53 (TP53 Human)
UseLentiviralTagsmCeruleanExpressionMammalianMutation7 silent mutations in p53 DNA to prevent targetin…PromoterEF1alphaAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
vYM112_PB-pEF1a-optiPIN2-HA(Hygro)
Plasmid#160045PurposeFor expressing the active auxin pump PIN2DepositorInsertPIN2
UseSynthetic BiologyTagsHAExpressionMammalianMutationcodon optimizedPromoterpEF1sAvailable sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP109 pEF1a-gRNA-GFP
Plasmid#100935PurposeGFP, puromycin and gRNA scaffold driven by EF1a promoterDepositorInsertgRNA scaffold
UseGrna scaffoldTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRRLNeo-pEF1a-p53ashL344A-mCerulean
Plasmid#69580PurposeExpresses p53-L344A mutant tagged with mCeruleanDepositorInsertp53-L344A (TP53 Human)
UseLentiviralTagsmCeruleanExpressionMammalianMutationp53 L344A mutation that prevents tetramerization …PromoterEF1alphaAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-Cre-PGK-Puro
Plasmid#207742PurposeCo-expression of the Cre recombinase and a puro selectable markerDepositorInsertCre-PGK-Puro
UseCre/LoxTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEF1α-squirrel-monkey-retroCHMP3-HA
Plasmid#154174Purposeexpresses squirrel monkey retroCHMP3 in mammalian cellsDepositorInsertretroCHMP3
UseTagsHAExpressionMammalianMutationPromoterEF1-alphaAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRRLNeo-pEF1a-p53ashL344P-mCerulean
Plasmid#69581PurposeExpresses p53-L344P mutant tagged with mCeruleanDepositorInsertp53-L344P (TP53 Human)
UseLentiviralTagsmCeruleanExpressionMammalianMutationp53 L344P mutation that prevents oliogomerization…PromoterEF1alphaAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pEF1a-DIO-tTAc
Plasmid#172126PurposeExpression of InteinC-tTAC in Cre recombinase positive cellsDepositorInsertInteinC-tTAc
UseAAVTagsExpressionMutationPromoterCaMKIIAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-M2rtTA-T2A-Puro
Plasmid#169175PurposepiggyBac co-transfection transposon containing M2rtTA promoter linked with Puromycin resistance gene, through T2A with GSG linker, subcloned between ends of two ITRs. Pairs with #169174DepositorInsertM2rtTA-T2A-Puro
UsePiggybac transposonTagsExpressionMammalianMutationSee Depositor Comments BelowPromoterEF1aAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-spider-monkey-retroCHMP3-HA
Plasmid#154179Purposeexpresses spider monkey retroCHMP3 in mammalian cellsDepositorInsertretroCHMP3
UseTagsHAExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only