We narrowed to 57,437 results for: cloning

1. pOTF3-AK (clone3)

   Plasmid

   #21165

   Depositor

   James Thomson

   Article

   Zwaka et al Nat Biotechnol. 2003 Mar . 21(3):319-21.

   Insert

   POUF5F1 (genomic DNA) (POU5F1 Human)
   

   Use

   Cre/Lox; Homologous recombination

   Tags

   Expression

   Mutation

   NeoR, EGFP, and SV40 polyA are between the two ge…

   Promoter

   Available since

   June 25, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC57-BBclone1

   Plasmid

   #137069

   Purpose

   E. coli plasmid that contains the expression optimized genes for BB0405, BB0406, P66, BBA57, HtrA, RevA and BB0238

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Inserts

   BB0405 (BB_0405 Borrelia burgdorferi B31)
   BB0406
   P66
   BBA57
   HtrA
   RevA (revA Borrelia burgdorferi B31)
   BB0238
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   wild-type, full-length (signal-peptide-containing…

   Promoter

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. gRNA_Cloning Vector BbsI

   Plasmid

   #128433

   Purpose

   Embty backbone vector for expression of gRNA for spCas9 genome editing or epigenome editing

   Depositor

   Izuho Hatada

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAT9624-BEAR-cloning

   Plasmid

   #162986

   Purpose

   BEAR target cloning plasmid with split EGFP

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   exchangeable cassette
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. [UBC][EIF4e][GG cloning]

   Plasmid

   #97189

   Purpose

   EIF4e plasmid with Pum cloning site

   Depositor

   Edward Boyden

   Article

   Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Insert

   EIF4E variant 1 (EIF4E Human)
   

   Use

   Synthetic Biology

   Tags

   empty Pum cloning site

   Expression

   Mammalian

   Mutation

   Promoter

   UBC (ubiquitin C)

   Available since

   Nov. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. 8xCTS1_BpiI-cloning-site_SV40polyA

   Plasmid

   #124547

   Purpose

   Destination cloning vector for tRNA-flanked sgRNA. A BpiI placeholder is present between a minimal ADE promoter and SV40 polyA sequence.

   Depositor

   Tudor Fulga

   Article

   Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiCRISPR v2-sgACSL4 clone2

   Plasmid

   #162129

   Purpose

   Lentiviral sgRNA plasmid targeting human ACSL4

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgACSL4 (ACSL4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. LentiCRISPR v2-sgACSL4 clone1

   Plasmid

   #162128

   Purpose

   Lentiviral sgRNA plasmid targeting human ACSL4

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgACSL4 (ACSL4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. BFP dest clone

   Plasmid

   #71825

   Purpose

   Lentiviral introduction of BFP reporter (Point mutation H66 in mEGFP results in BFP expression)

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. LentiCRISPR v2-sgLKB1 clone2

    Plasmid

    #162126

    Purpose

    Lentiviral sgRNA plasmid targeting human LKB1

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgLKB1 (STK11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. LentiCRISPR v2-sgLKB1 clone1

    Plasmid

    #162125

    Purpose

    Lentiviral sgRNA plasmid targeting human LKB1

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgLKB1 (STK11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. LentiCRISPR v2-sgLKB1 clone3

    Plasmid

    #162127

    Purpose

    Lentiviral sgRNA plasmid targeting human LKB1

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgLKB1 (STK11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. LentiCRISPR v2-sgACSL4 clone3

    Plasmid

    #162130

    Purpose

    Lentiviral sgRNA plasmid targeting human ACSL4

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgACSL4 (ACSL4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pst39-NST-clone14

    Plasmid

    #133421

    Purpose

    S. cerevisiae Nip1 (with C-terminal TEV cleavage site and strep-II tag), Sui1 and Tif5B6 (with N-terminal 7xHis tag and TEV cleavage set) coding sequences with intervening ribosome binding sites and other cis-elements for expression as a polycistron, T7 theta 10 promoter (and corresponding terminator after polycistron).

    Depositor

    Gerhard Wagner

    Insert

    NIP1/SUI1/TIF5 (NIP1, TIF5, SUI1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    ORF for Nip1 encodes aa 4-156 (not complete ORF),…

    Promoter

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Kek1 (Lip1) clone #1

    Plasmid

    #38750

    Depositor

    Norbert Perrimon

    Article

    Musacchio et al Dev Biol. 1996 Aug 25;178(1):63-76.

    Insert

    Kek1 (kek1 Fly)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pBS hTSP2 clone M1-B

    Plasmid

    #12412

    Depositor

    Paul Bornstein

    Insert

    M1-B TSP2 (THBS2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Fragment from Heparin Binding domain and Type 1 r…

    Promoter

    Available since

    Oct. 23, 2006

    Availability

    Academic Institutions and Nonprofits only

17. Bison sgRNA library

    Pooled Library

    #169942

    Purpose

    The BISON CRISPR library targets 713 E1, E2, and E3 ubiquitin ligases, deubiquitinases, and control genes and contains 2,852 guide RNAs. It was cloned into the pXPR003.

    Depositor

    Benjamin Ebert

    Article

    Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:

    Use

    Lentiviral

    Available since

    Sept. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

18. pLKO.1 - TRC cloning vector

    Plasmid

    #10878

    Purpose

    3rd generation transfer plasmid

    Depositor

    David Root

    Article

    Moffat et al Cell. 2006 Mar 24. 124(6):1283-98.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. sgRNA(MS2) cloning backbone

    Plasmid

    #61424

    Purpose

    sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCS2+mNeonGreen-C Cloning Vector

    Plasmid

    #128144

    Purpose

    To express a protein of interest fused to the C-terminus of mNeonGreen

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Use

    Tags

    mNeonGreen fusion

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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