Addgene

We narrowed to 1,131 results for: fret

Showing: 61 - 80 of 1131 results

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mPA-GFP-mPlum-FRET-10

Plasmid

#58154

Purpose

Localization: Cytosolic FRET, Excitation: 504 / 590, Emission: 517 / 649

Depositor

Michael Davidson

Type

Empty backbone

Use

Tags

mPA-GFP and mPlum

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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mPA-GFP-tdTomato-FRET-10

Plasmid

#58155

Purpose

Localization: Cytosolic FRET, Excitation: 504 / 554, Emission: 517 / 581

Depositor

Michael Davidson

Type

Empty backbone

Use

Tags

mPA-GFP and tdTomato

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only
Combined γB2FL-FRET probe

Plasmid

#209094

Purpose

mTurquoise2- and mVenus-inserted PcdhgB2 full length (FL)

Depositor

Takeharu Nagai

Article

Hoshino et al Proc Natl Acad Sci U S A. 2023 Sep 19;12

Insert

PcdhgB2 (Pcdhgb2 Mouse)

Use

Tags

PA tag

Expression

Mammalian

Mutation

Promoter

Chicken β-actin

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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Combined γB2ΔICD-FRET probe

Plasmid

#209093

Purpose

mTurquoise2- and mVenus-inserted PcdhgB2 lacking an intracellular domain (ICD)

Depositor

Takeharu Nagai

Article

Hoshino et al Proc Natl Acad Sci U S A. 2023 Sep 19;12

Insert

PcdhgB2 (Pcdhgb2 Mouse)

Use

Tags

PA tag

Expression

Mammalian

Mutation

Promoter

Chicken β-actin

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pFR-mScarletI-antiFRET-P2A-G-Ca-FLITS

Plasmid

#191457

Purpose

Compare the intensity of the genetically encoded calcium sensor (GECI) G-Ca-FLITS to mScarlet-I fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

G-Ca-FLITS

Use

Tags

His-mScarletI-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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inactive cdk1 FRET sensor (2328)

Plasmid

#26065

Depositor

Jonathon Pines

Article

Gavet et al Dev Cell. 2010 Apr 20. 18(4):533-43.

Insert

Cdk1 FRET Sensor- Inactive

Use

Tags

CFP variant, FLAG, and YFP variant

Expression

Mammalian

Mutation

Ser 126 mutated to Ala

Promoter

Available since

Sept. 15, 2010

Availability

Academic Institutions and Nonprofits only
Plk1 FRET sensor c-jun substrate

Plasmid

#45203

Depositor

Michael Lampson

Article

Liu et al J Cell Biol. 2012 Aug 20;198(4):491-9. d

Insert

Plk1 FRET sensor with c-jun substrate

Use

Tags

CyPET, YPET

Expression

Mammalian

Mutation

Promoter

T7

Available since

June 26, 2013

Availability

Academic Institutions and Nonprofits only

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PLK1-cjun-FRET-PACT biosensor

Plasmid

#106907

Purpose

centrosome-localized PLK1 FRET-activity biosensor (through PACT domain with 10aa linker)

Depositor

Heidi Hehnly

Article

Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

Insert

PLK1-cjun-FRET-PACT biosensor with linker (PLK1 Human)

Use

Tags

PACT domain

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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GgVcl 1-1066 FRET tail probe 1

Plasmid

#46276

Depositor

Susan Craig

Article

Chen et al J Cell Biol. 2005 May 9;169(3):459-70.

Insert

Vcl 1-1066 + EYFP (VCL Chicken)

Use

Tags

ECFP

Expression

Mammalian

Mutation

addition of 9 bp linker and EYFP at aa 883

Promoter

CMV

Available since

July 11, 2013

Availability

Academic Institutions and Nonprofits only

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Mis12-targted Aurora B FRET sensor

Plasmid

#45231

Depositor

Michael Lampson

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

Mis12 targeted Aurora B FRET sensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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pFR-mTq2-antiFRET-P2A-jRCaMP1b

Plasmid

#191468

Purpose

Compare the intensity of the genetically encoded calcium sensor (GECI) jRCaMP1b to mTurquoise2 fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

jRCaMP1b

Use

Tags

His-mTurquoise2-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Cenp-B-targeted Aurora B FRET sensor

Plasmid

#45232

Depositor

Michael Lampson

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

CENP-B targeted Aurora B FRET sensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

t7

Available since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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pmCIT-CFPGgVcl 1-419 control 2 FRET

Plasmid

#46283

Depositor

Susan Craig

Article

Chen et al J Biol Chem. 2006 Dec 29;281(52):40389-9

Insert

ECFP + Vcl 1-419 (VCL Chicken)

Use

Tags

mCitrine

Expression

Mammalian

Mutation

aa 1-419

Promoter

CMV

Available since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only

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GgVcl 1-1066 FRET tail probe 2

Plasmid

#46282

Depositor

Susan Craig

Article

Chen et al J Biol Chem. 2006 Dec 29;281(52):40389-9

Insert

Vcl 1-1066 + mCitrine (VCL Chicken)

Use

Tags

ECFP

Expression

Mammalian

Mutation

mCitrine (and linker) inserted after aa883

Promoter

CMV

Available since

July 11, 2013

Availability

Academic Institutions and Nonprofits only

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pFR-mTq2-antiFRET-P2A-RCaMP1h

Plasmid

#191467

Purpose

Compare the intensity of the genetically encoded calcium sensor (GECI) RCaMP1h to mTurquoise2 fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

RCaMP1h

Use

Tags

His-mTurquoise2-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pFR-mScarletI-antiFRET-P2A-EGFP

Plasmid

#191458

Purpose

Compare the intensity of EGFP to mScarlet-I fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

EGFP

Use

Tags

His-mScarletI-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pFR-mScarletI-antiFRET-P2A-mTurquoise2_T203Y

Plasmid

#191459

Purpose

Compare the intensity of mTurquoise2_T203Y to mScarlet-I fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

mTurquoise2_T203Y

Use

Tags

His-mScarletI-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pFR-mTq2-antiFRET-P2A-mCherry

Plasmid

#191466

Purpose

Compare the intensity of mCherry to mTurquoise2 fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

mCherry

Use

Tags

His-mTurquoise2-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pFR-mScarletI-antiFRET-P2A-GCaMP3

Plasmid

#191460

Purpose

Compare the intensity of the genetically encoded calcium sensor (GECI) GCaMP3 to mScarlet-I fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

GCaMP3

Use

Tags

His-mScarletI-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pFR-mTq2-antiFRET-P2A-K-GECO1

Plasmid

#191469

Purpose

Compare the intensity of the genetically encoded calcium sensor (GECI) K-GECO1 to mTurquoise2 fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

K-GECO1

Use

Tags

His-mTurquoise2-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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