-
Plasmid#207020PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
UseTagsGFPExpressionYeastMutationI375APromoterpTEF1Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-W371A-GFP
Plasmid#207019PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
UseTagsGFPExpressionYeastMutationW371APromoterpTEF1Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-TRP1-TetOff(7repeats)-GFP-Pho8
Plasmid#207011PurposeTet-Off controlled GFP-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.DepositorInsertPho8
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-3C-sGFP1-10_pcDNA3.1(-)
Plasmid#182931PurposeFor expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragmentDepositorTypeEmpty backboneUseTags3C-cleavable split GFP 1-10, FLAG tag, and HA sig…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTub-alpha-1-mtGFP
Plasmid#138981PurposeNeuron-specific expression of membrane targeted EGFPDepositorInsertEGFP
UseTagsExpressionMammalianMutationEGFP (added membrane targeting domain from GAP43).PromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
MscL(G22S)61-cpGFP-ERexp
Plasmid#195311PurposeFluorescent reporter/biosensor for membrane tensionDepositorInsertMscL(G22S)61-cpGFP-ERexp
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRB33-GFP
Plasmid#165483PurposepTriEx based vector for protein expression in mammalian cells for targets fused with N-terminal uv-GFP, Flag and HisDepositorTypeEmpty backboneUseTags10xHis-FLAG-GFP-TEVExpressionMammalianMutationPromoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pclbw-eGFPomp25
Plasmid#58424PurposeMammalian expression of GFP targeted to the mitochondrial outer membraneDepositorInserteGFP-omp25 (Synj2bp Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationcontains aa's 109-145 of mouse omp25PromoterCMV/b-actinAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pclbw-PAGFPomp25
Plasmid#58427PurposeMammalian expression of photoactivatable GFP targeted to the mitochondrial outer membraneDepositorInsertpaGFP-omp25 (Synj2bp Mouse)
UseRetroviralTagsPAGFPExpressionMammalianMutationcontains aa's 109-145 of mouse omp25Promotercmv-bactinAvailable sinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-D384A Y387A-GFP
Plasmid#207024PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
UseTagsGFPExpressionYeastMutationD384A Y387APromoterpTEF1Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-W371A I375A-GFP
Plasmid#207023PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
UseTagsGFPExpressionYeastMutationW371A I375APromoterpTEF1Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Lyn11C32S-PIP4K2A
Plasmid#202748Purposeweakly plasma membrane targeted fluorescent protein-tagged enzymeDepositorInsertEGFP:LYN (1-11) (C3S):PIP4K2A (PIP4K2A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_shRNA resistant nmPKA-Cα-mEGFP
Plasmid#114144PurposeThe G2A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.DepositorInsertmouse PKA Cα-mEGFP with G2A mutation and silent mutation to resist shRNA knockdown (Prkaca Mouse)
UseTagsExpressionMammalianMutationG2A mutation and silent mutation to resist shRNA …PromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
pRB30-GFP
Plasmid#165481PurposepTriEx based vector for protein expression in mammalian cells for targets fused with C-terminal uv-GFP, Flag and HisDepositorTypeEmpty backboneUseTagsTEV-GFP-FLAG-10x HisExpressionMammalianMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP G protein Gamma 3(F70A,F71A) in pcDNA3.1
Plasmid#42185DepositorInsertG protein Gamma 3(F70A, F71A) (Gng3 Mouse)
UseTagsEGFPExpressionMammalianMutationF70A, F71APromotercmvAvailable sinceMarch 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
EGFP G protein Gamma 3(K68A,K69A) in pcDNA3.1
Plasmid#42188DepositorInsertGamma 3(K68A,K69A) (Gng3 Mouse)
UseTagsEGFPExpressionMammalianMutationK68A,K69APromotercmvAvailable sinceMarch 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
GFP-C1-PLCdelta-PH
Plasmid#21179Purposeencodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipidsDepositorInsertPLCdelta (PLCD1 Human)
UseTagsGFPExpressionMammalianMutationPH domain of human PLC delta, amino acids Met1–Il…PromoterAvailable sinceJuly 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
GFP-LiMETER-v2
Plasmid#113934PurposeAlso termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFPDepositorInsertLOV2 and Stim1 polybasic domain (PB)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lyn11C3S-EGFP
Plasmid#202747Purposeweakly plasma membrane targeted fluorescent proteinDepositorInsertLYN (1-11) (C3S):EGFP (LYN Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only