Addgene

We narrowed to 2,073 results for: TRIM

Showing: 1561 - 1600 of 2073 results

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pcDNA3.1-mas-p115RH-Nluc

Plasmid

#158607

Purpose

Plasmid expressing p115(RH)-Nluc in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - p115RhoGEF RH domain - linker (GGTGGS) - Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-DsRed_IRES_EGFP

Plasmid

#92194

Purpose

Lentiviral overexpression vector to make stable bicistronic cell line for control (EGFP) screen

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

DsRed-IRES-EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-ATG5

Plasmid

#99573

Purpose

Expresses Cas9 with sgRNA targeting Exon 7 of ATG5

Depositor

Edward Campbell

Article

Imam et al J Virol. 2016 Jan 13. pii: JVI.03033-15.

Insert

gRNA targeting ATG5 (ATG5 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a

Plasmid

#25030

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DCP1a (DCP1A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 23, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hDDX41-HA

Plasmid

#165469

Purpose

Human DDX41 mammalian expression vector with C-terminal HA tag.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

DEAD-box helicase 41 (DDX41 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRCK

Plasmid

#25033

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

Rck (DDX6 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2010

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsEDC3

Plasmid

#25032

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

EDC3 (EDC3 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV3FLAG_hGTPBP4

Plasmid

#53974

Purpose

Mammalian expression plasmid for human GTPBP4 with n-terminal 3xFLAG epitope fused.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

GTPBP4 (GTPBP4 Human)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

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strep-IRF8

Plasmid

#102872

Purpose

Express strep-tagged mouse IRF8 protein in E. coli

Depositor

Gary Stormo

Article

Chang et al BMC Mol Biol. 2018 Mar 27;19(1):5. doi:

Insert

IRF8 (Irf8 Mouse)

Use

Tags

strep tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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mouse CENP-B-EGFP

Plasmid

#107264

Purpose

mouse CENP-B-EGFP in pIVT vector for in vitro transcription

Depositor

Michael Lampson

Article

Akera et al Science. 2017 Nov 3;358(6363):668-672. d

Insert

CENP-B-EGFP (Cenpb Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

T7

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hDDX50-HA

Plasmid

#165465

Purpose

Human DDX50 mammalian expression vector with C-terminal HA tag.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

DExD-box helicase 50 (DDX50 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCMVhPOP1_3Flag

Plasmid

#53968

Purpose

Mammalian expression vector for human POP1 with c-terminal 3xFLAG epitopes.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

POP1 (POP1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

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pCS2+PhyBΔCreC

Plasmid

#131781

Purpose

Mammalian, Zebrafish, or Xenopus expression of PhyBΔCreC, one half of the CreLite system.

Depositor

George Eisenhoffer

Article

Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

Insert

PhyBCreC

Use

Cre/Lox; Zebrafish expression

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pME-CreLite

Plasmid

#131782

Purpose

Middle entry vector (Tol2 kit) with bi-cistronic CreLite system components, PhyBΔCreC and PIF6CreN, linked by P2A.

Depositor

George Eisenhoffer

Article

Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

Insert

PhyBCreC-P2A-PIF6CreN

Use

Cre/Lox; Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CreLite

Plasmid

#131786

Purpose

Lentiviral vector expressing CreLite, driven by CBh promoter

Depositor

George Eisenhoffer

Article

Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

Insert

PhyBCreC-P2A-PIF6CreN

Use

Cre/Lox, Lentiviral, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAH18

Plasmid

#154397

Purpose

Derived from pGPI-SceI; Trimethoprim resistant; carries I-SceI gene; contains 475 bp from both upstream and downstream flanking regions between the FRT sites on pAHCTX1-rhadCas9

Depositor

Silvia Cardona

Article

Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

Insert

Fused upstream and downstream homologous regions around FRT sites when pAH-CTX1-rhadCas9 integrated into K56-2 genome

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

NA

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hHP1BP3-HA

Plasmid

#165468

Purpose

Human HP1BP3 mammalian expression vector with C-terminal HA tag.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

Heterochromatin protein 1 binding protein 3 (HP1BP3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-Gαi3-YFP (Citrine variant, b/c loop insert)

Plasmid

#171375

Purpose

For the mammalian expression of rat Gαi3 internally tagged with YFP

Depositor

Mikel Garcia-Marcos

Article

Marivin et al Sci Signal. 2016 Apr 12;9(423):ra37. doi

Insert

Gai3-YFP

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mas-KB-1753-Nluc I9A/W10A

Plasmid

#158604

Purpose

Plasmid expressing KB-1753-Nluc I9A/W10A in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 I9A/W10A - linker (GGTGGS) - Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-mas-GRK2RH-Nluc L118A

Plasmid

#158606

Purpose

Plasmid expressing GRK2(RH)-Nluc L118A in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - GRK2 RH domain L118A - linker (GGTGGS) - Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-PML B-box1 WT

Plasmid

#185632

Purpose

expression of GST-tagged wild-type PML B-box1 domain in bacterial cells

Depositor

Irina Bezsonova

Article

Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

Insert

promyelocytic leukemia protein (PML Human)

Use

Tags

GST

Expression

Bacterial

Mutation

none

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-PML B-box1 C4C4

Plasmid

#185640

Purpose

expression of GST-tagged mutant PML B-box1 H155C/H161C domain in bacterial cells

Depositor

Irina Bezsonova

Article

Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

Insert

promyelocytic leukemia protein (PML Human)

Use

Tags

GST

Expression

Bacterial

Mutation

H155C H161C

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-PML B-box1 W157E

Plasmid

#185641

Purpose

expression of GST-tagged mutant PML B-box1 W157E domain in bacterial cells

Depositor

Irina Bezsonova

Article

Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

Insert

promyelocytic leukemia protein (PML Human)

Use

Tags

GST

Expression

Bacterial

Mutation

W157E

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-PML B-box1 F158E

Plasmid

#185642

Purpose

expression of GST-tagged mutant PML B-box1 F158E domain in bacterial cells

Depositor

Irina Bezsonova

Article

Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

Insert

promyelocytic leukemia protein (PML Human)

Use

Tags

GST

Expression

Bacterial

Mutation

F158E

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C1-IFT57

Plasmid

#218728

Purpose

Expresses N-terminally EGFP-tagged IFT57 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Funabashi et al J Cell Biol. 2018 Aug 6;217(8):2867-2876

Insert

IFT57 (IFT57 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsDCP1a-withDmTD_H

Plasmid

#146504

Purpose

Mammalian Expression of HsDcp1a-withDmTD

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDcp1a-withDmTD (DCP1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsDCP1a-withDmTD_H

Plasmid

#146506

Purpose

Mammalian Expression of HsDCP1a-withDmTD

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDCP1a-withDmTD (DCP1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSFDuetG-HsDCP1a_539-582 (MCSII)-mut1_G

Plasmid

#146408

Purpose

Bacterial Expression of HsDCP1a_539-582-mut1

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDCP1a_539-582-mut1 (DCP1A Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSFDuetG-HsDCP1a_539-582 (MCSII)-mut3_G

Plasmid

#146410

Purpose

Bacterial Expression of HsDCP1a_539-582-mut3

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDCP1a_539-582-mut3 (DCP1A Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsDCP1a_539-582_G

Plasmid

#146412

Purpose

Mammalian Expression of HsDcp1a_539-582

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDcp1a_539-582 (DCP1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsDCP1a-del155-168_G

Plasmid

#146413

Purpose

Mammalian Expression of HsDcp1a-del155-168

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDcp1a-del155-168 (DCP1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsDCP1a-del539-582_G

Plasmid

#146414

Purpose

Mammalian Expression of HsDcp1a-del539-582

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDcp1a-del539-582 (DCP1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsDCP1a-mut2_G

Plasmid

#146416

Purpose

Mammalian Expression of HsDCP1a-mut2

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDCP1a-mut2 (DCP1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsDCP1a-mut3_G

Plasmid

#146417

Purpose

Mammalian Expression of HsDCP1a-mut3

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDCP1a-mut3 (DCP1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsDCP1a-mut1_G

Plasmid

#146415

Purpose

Mammalian Expression of HsDCP1a-mut1

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDCP1a-mut1 (DCP1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-His-hUbi

Plasmid

#31815

Depositor

Astar Winoto

Article

Young et al J Biol Chem. 2011 Feb 25;286(8):6521-31.

Insert

Ubiquitin (UBB Human)

Use

Tags

HIS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV3Tag8li-hADARB2-3xFLAG

Plasmid

#165470

Purpose

Human ADARB2 mammalian expression vector with 3 C-terminal FLAG tags.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

Adenosine deaminase RNA specific B2 (inactive) (ADARB2 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-DsRed_IRES_MAPT:EGFP

Plasmid

#92196

Purpose

Lentiviral overexpression vector to make stable bicistronic cell line for Tau screen

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

DsRed-IRES-MAPT-EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-DsRed_IRES_SNCA:EGFP

Plasmid

#92195

Purpose

Lentiviral overexpression vector to make stable bicistronic cell line for α-Syn screen

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

DsRed-IRES-SNCA-EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only
pGEX-6P-1-INTS3-FL

Plasmid

#128415

Purpose

Express INTS3 full-length in E. coli with a GST tag (N-terminal)

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

Insert

INTS3 (INTS3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

full-length

Promoter

tac promoter

Available since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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