We narrowed to 1,262 results for: grna cloning vector

1. pSI-218

   Plasmid

   #197139

   Purpose

   All-in-vector of SpCas9 gRNA cloning sites (BsmBI) and CMVp-Target-AID expression unit

   Depositor

   Nozomu Yachie

   Article

   Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   SpCas9-PmCDA1-UGI

   Expression

   Mammalian

   Mutation

   SpCas9(D10A)

   Promoter

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAGC-Lb12-P1Bb

   Plasmid

   #231387

   Purpose

   For crRNA cloning with LbCas12a vectors for genome editing in Arabidopsis

   Depositor

   Qi-Jun Chen

   Article

   Xin et al aBIOTECH. 2024 Mar 26;5(2):117-126. doi:

   Insert

   U6-29
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCRISPRi_Mxi1_yl

   Plasmid

   #91248

   Purpose

   CRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertion

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

   Inserts

   Codon optimized dCas9-Mxi1
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SCR1'-tRNA and UAS1B8-TEF(136)

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti_MCP-p300_Hygro

   Plasmid

   #138459

   Purpose

   lenti vector encoding the MS2-p300 with a 2A Hygro resistance marker

   Depositor

   Jian Xu

   Article

   Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

   Insert

   p300 core
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPRi_Mxi1_yl_NHEJ

   Plasmid

   #91249

   Purpose

   CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

   Inserts

   Codon optimized dCas9-Mxi1
   KU70 sgRNA expression cassette
   KU80a sgRNA expression cassette
   KU80b sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SCR1'-tRNA and UAS1B8-TEF(136)

   Available since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pV1382

   Plasmid

   #111436

   Purpose

   S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPRIME-TET-GFP-FF3

   Plasmid

   #11662

   Purpose

   3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a tetracycline-responsive promoter (TET) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

   Insert

   FF3
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 1, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPRIME-CMV-GFP-FF3

   Plasmid

   #11663

   Purpose

   3rd generation lentiviral transfer vector. pPRIME cloning plasmid with CMV promoter controlling expression of GFP and miR30-based shRNA targeting firefly luciferase

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

   Insert

   FF3
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 1, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pV1326

   Plasmid

   #111435

   Purpose

   S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPRIME-CMV-dsRed-FF3

    Plasmid

    #11664

    Purpose

    3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a CMV promoter controlling expression of dsRed and miR30-based shRNA targeting firefly luciferase

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Insert

    FF3
    

    Use

    Lentiviral

    Tags

    dsRed

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pVG1

    Plasmid

    #111444

    Purpose

    Unified Solo vector pV1382 + sgScADE2 + ScADE2 stop codon repair tempate

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgScADE2/stop-codon repair
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPRIME-CMV-LNGFR-FF3

    Plasmid

    #11666

    Purpose

    3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a CMV promoter controlling expression of LNGFR and miR30-based shRNA targeting firefly luciferase

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Insert

    FF3
    

    Use

    Lentiviral

    Tags

    LNGFR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pV1539

    Plasmid

    #111434

    Purpose

    Genedrive entry vector for Candida albicans

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPRIME-CMV-Neo-FF3

    Plasmid

    #11665

    Purpose

    3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a CMV promoter controlling expression of Neomycin and miR30-based shRNA targeting firefly luciferase

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Insert

    FF3
    

    Use

    Lentiviral

    Tags

    Neo

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pV1464

    Plasmid

    #111440

    Purpose

    N. castellii Solo CRISPR vector, marked with URA3 and NatR

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MS2-adRNA (5'UAG)

    Plasmid

    #170126

    Purpose

    Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    MS2-ADAR2(5'UAG)-MS2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 and mouse U6

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPRIME-TREX-GFP-FF3

    Plasmid

    #11667

    Purpose

    3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a Tet-responsive promoter (TREX) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Insert

    FF3
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCFD3-MS2tail-SAM

    Plasmid

    #78905

    Purpose

    Vector for expressing a single sgRNA with SAM MS2 tail, under U6:3 promoter

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MS2-adRNA (3'UAG)

    Plasmid

    #170128

    Purpose

    Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    MS2-ADAR2(3'UAG)-MS2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 and mouse U6

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCFD3-MS2tail-Scaffold

    Plasmid

    #78906

    Purpose

    Vector for expressing a single sgRNA with Scaffold MS2 tail, under U6:3 promoter

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge