Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 4,240 results for: spl

Clear keywords
Showing: 781 - 800 of 4240 results

  2. pU6_(GLuc)_INT[3xMS2_SL]

    Plasmid
    #68426
    Purpose
    Transient expression in mammalian cells of an "INT" construct_bearing three MS2 Stem-loops, targeting the GLuc reporter. U6 promoter
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    INT construct_bearing three MS2 Stem-loops
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68426-plasmid-map-sequence-id-125556

    Enlarge

  11. pB2-neo-TET

    Plasmid
    #51287
    Purpose
    expresses the rodent SINE, B2, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassette
    Depositor
    Astrid Roy-Engel
    Publication
    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:
    Insert
    sine B2
    Tags
    neoTET cassette with a tetrahymena self-splicing …
    Expression
    Mammalian
    Promoter
    enhancer from 7SL gene
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    51287-plasmid-map-sequence-id-78369

    Enlarge

  12. TDP-REGv2(mScarlet-FLAG)#10 in pTwist-CMV backbone

    Plasmid
    #216154
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. Leaky but very sensitive to mild TDP-43 loss of function. It uses a cryptic exon. [Code 'B5'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Publication
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mScarlet with cryptic exon and C-terminal FLAG
    Expression
    Mammalian
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    216154-plasmid-map-sequence-id-437765

    Enlarge

  13. TDP-REGv2(mScarlet-FLAG) matched positive control in pTwist-CMV

    Plasmid
    #216156
    Purpose
    Expresses mScarlet regardless of TDP-43 knockdown. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Publication
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mScarlet with C-terminal FLAG tag
    Expression
    Mammalian
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216156-plasmid-map-sequence-id-437768

    Enlarge

  14. EF1a-L274GMmMetRS-T2A-mCherry

    Plasmid
    #220800
    Purpose
    Lentivirus expressing mutant tRNA synthetase for incorporation of noncanonical amino acids in nascent proteins plus mCherry reporter and puro resistance
    Depositor
    Elizabeth Kirby
    Publication
    Sridharan et al Stem Cell Res Ther. 2024 Jun 26;15(1):18
    Insert
    Mars1 (Mars1 Mouse)
    Use
    Lentiviral
    Tags
    2xFLAG and T2A-mCherry
    Mutation
    mutation in MetRS to change aa 274 from L to G
    Promoter
    EF1a
    Available since
    June 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    220800-plasmid-map-sequence-id-437285

    Enlarge

  15. pU6_(GLuc)_INT[3xPP7_SL]

    Plasmid
    #68424
    Purpose
    Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U6 promoter
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    INT construct bearing three PP7 Stem-loops
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68424-plasmid-map-sequence-id-125555

    Enlarge

  16. PB-CAG-dCasRx-RBM25-IRES-BleoR

    Plasmid
    #221002
    Purpose
    Stable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.
    Depositor
    Benjamin Blencowe, Mikko Taipale
    Publication
    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCasRx-RBM25-IRES-BleoR
    Use
    CRISPR
    Tags
    NLS and NLS-HA
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    221002-plasmid-map-sequence-id-436162

    Enlarge

  18. Yeast MoClo Secretion and Surface Display (YSD) Toolkit 2.0

    Plasmid kit
    #1000000290
    Purpose
    Toolkit to optimize secretion and cell surface display of proteins of interest in Saccharomyces cerevisiae, intended for creating whole-cell biocatalysts and analyzing of protein–protein interactions.
    Depositor
    Paul Young
    Application
    Cloning and Synthetic Biology
    Vector type
    Yeast Expression
    Cloning type
    Golden Gate (MoClo)
    Available since
    July 24, 2026
    Availability
    Academic Institutions and Nonprofits only

  19. pDN-D2irTNG4kwh

    Plasmid
    #44722
    Depositor
    Gabor Balazsi
    Publication
    Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    pCMV-D2i promoter
    htetR::NLS::EGFP
    Use
    Synthetic Biology; Expression regulator/reporter;…
    Tags
    Rabbit β-globin intron II and WPRE
    Expression
    Mammalian
    Mutation
    Initiator motif (Inr) displaced relative to pCMV-…
    Promoter
    pCMV-D2i
    Available since
    April 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    44722-plasmid-map-sequence-id-61263

    Enlarge

  20. T2A Split/Link TurboID Kit

    Plasmid kit
    #1000000249
    Purpose
    Modular assembly of expression vectors for proximity labeling experiments.
    Depositor
    Sven Eyckerman
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector type
    Lentiviral, Mammalian Expression
    Cloning type
    Golden Gate
    Available since
    Aug. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 781 - 800 of 4240 results