We narrowed to 20,261 results for: INO
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Plasmid#202441PurposeExpression of a guide RNA targeting KCMF1DepositorAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only
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pAAV-nEF-Coff/Fon-Arch3.3-p2a-EYFP
Plasmid#137150PurposeIntersectional viral expression of Arch3.3-EYFP in cells expressing Flp AND NOT CreDepositorHas ServiceAAV8InsertCoff/Fon-Arch3.3-p2a-EYFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtMutationn/aPromoternEFAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
SOX2-P2A-tagBFP-HDR
Plasmid#163751PurposeHDR knock-in template for tagging the human endogenous SOX2 gene with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.DepositorInsertSOX2 (SOX2 Human)
UseCRISPR and Synthetic BiologyTagsP2A-tagBFPMutationDoes not contain start codon to avoid random inte…Available SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-eSpCas9-2A-GFP-sgRNA-HSD17B11
Plasmid#161923PurposeTo generate HSD17B11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against HSD17B11 exon 1.DepositorInsertsgRNA targeting HSD17B11 exon 1
UseCRISPRPromoterU6Available SinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSPL3-hRPH3A_c.1853Gmut
Plasmid#190479Purposeminigene assayDepositorInsertRPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)
ExpressionMammalianMutationc.1853A>GPromoterSV40Available SinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lentiviral click editor (CE1) plasmid - LTR-pEF1a-PCV2-nCas9-EcKlenow (DRR921)
Plasmid#217798PurposeLentiviral plasmid to produce the prototypical CE1 construct (PCV2-nCas9-EcKlenow), expressed from the EF1a promoterDepositorInsertXTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR and LentiviralTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); EcKlenow(-exo;D355A/E357A)PromoterEF1aAvailable SinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Foff 2.0-ChR2-mCherry
Plasmid#137143PurposeIntersectional viral expression of ChR2-mCherry in cells expressing Cre AND NOT FlpDepositorHas ServiceAAV8InsertCon/Foff 2.0-ChR2-mCherry
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtMutationH134RPromoternEFAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CARMA2sh-E138A
Plasmid#97068PurposeExpresses Psoriasis-Linked mutant CARD14/CARMA2 in mammalian cellsDepositorInsertCARD14 isoform 3 (CARD14 Human)
TagsHAExpressionMammalianMutationchanged Glutamic Acid 138 to AlaninePromoterCMVAvailable SinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOTTC292 - pAAV EF1a floxed hChR2(H134R)-EYFP
Plasmid#50834PurposeAn AAV packaging vector that expresses channel rhodopsin 2 (H134R) (fused to EYFP) under the EF1a promoter, which can be deactivated (deleted) by recombination between the flanking loxP sites.DepositorInserthChR2 (H134R)
UseAAV and Cre/LoxTagsEYFPExpressionMammalianMutationH134RPromoterEF1aAvailable SinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(GATA3(13))-PGKpuro2ABFP-W
Plasmid#208548PurposeLentiviral vector expressing gRNA targeting human GATA3DepositorInsertGATA3(13) (GATA3 Human)
UseLentiviralAvailable SinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(GATA3(12))-PGKpuro2ABFP-W
Plasmid#208547PurposeLentiviral vector expressing gRNA targeting human GATA3DepositorInsertGATA3(12) (GATA3 Human)
UseLentiviralAvailable SinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-opCas12a-Ultra-6xNLS
Plasmid#209024PurposeLentiviral vector expressing human codon-optimized opCas12a-Ultra with 6xNLSDepositorInsertopCas12a-Ultra-6xNLS-Flag-Myc-NeoR
UseCRISPR and LentiviralTagsFLAG and MYCExpressionMammalianMutationE174R/M537R/S542R/F870LPromoterEF-1aAvailable SinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
Snap25-LSL-2A-GFP targeting vector
Plasmid#61575PurposeTarget a Cre-dependent GFP expression cassette to the stop codon of the mouse Snap25 geneDepositorInsertSnap25-LSL-2A-GFP
UseCre/Lox and Mouse TargetingAvailable SinceFeb. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTRE-HRI-V5-IRES-BFP
Plasmid#202435PurposeLentiviral vector expression of HRI and TagBFPDepositorAvailable SinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-sgCD20
Plasmid#209749PurposeLentiviral transfer plasmid to express Cas9 and a gRNA targeting the human CD20 gene, MS4A1.DepositorInsertMS4A1 (MS4A1 Human)
UseLentiviralAvailable SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV pTRE-FSF-FLEX-EGFP-WPRE-bGHpA
Plasmid#65453PurposeCan be used to generate AAV virus that will express EGFP from the tetO promoter under intersectional control by Flp and Cre recombinasesDepositorHas ServiceAAV1InsertEGFP
UseAAVPromotertetOAvailable SinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCW57-Cx32-IRES-GCaMP6s
Plasmid#216795PurposeInducible bicistronic lentiviral plasmid for the simultaneous expression of Connexin 32 and cytosolic GCaMP6sDepositorInsertConnexin 32-IRES-GCaMP6s (GJB1 Human)
UseLentiviralAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
HUHe variant click editor (CE1) - pCMV-T7-TGMV-nCas9-EcKlenow (JO659)
Plasmid#208951PurposeA variant CE1 construct with TGMV HUH enconuclease, expressed from CMV or T7 promoters.DepositorInsertTGMV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); EcKlenow(-exo;D355A/E357A)PromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
HUHe variant click editor (CE1) - pCMV-T7-FBYNV-nCas9-EcKlenow (JO610)
Plasmid#208950PurposeA variant CE1 construct with FBYNV HUH endonuclease, expressed from CMV or T7 promoters.DepositorInsertFBYNV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); EcKlenow(-exo;D355A/E357A)PromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
HUHe variant click editor (CE1) - pCMV-T7-RepBM-nCas9-EcKlenow (JO606)
Plasmid#208949PurposeA variant CE1 construct with RepBM HUH endonuclease, expressed from CMV or T7 promoters.DepositorInsertRepBm-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); EcKlenow(-exo;D355A/E357A)PromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only