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pYTK074

Plasmid

#65181

Purpose

Encodes URA3 as a Type 6 part to be used in the Dueber YTK system

Depositor

John Dueber

Publication

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

URA3

Expression

Bacterial

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK056

Plasmid

#65163

Purpose

Encodes tTDH1 as a Type 4 part to be used in the Dueber YTK system

Depositor

John Dueber

Publication

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

tTDH1

Expression

Bacterial

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK027

Plasmid

#65134

Purpose

Encodes pREV1 as a Type 2 part to be used in the Dueber YTK system

Depositor

John Dueber

Publication

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

pREV1

Expression

Bacterial

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pYTK009

Plasmid

#65116

Purpose

Encodes pTDH3 as a Type 2 part to be used in the Dueber YTK system

Depositor

John Dueber

Publication

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

pTDH3

Expression

Bacterial

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pExo1

Plasmid

#53357

Purpose

Expresses beta-lactamase for the degradation of beta-lactam antibiotics and tags it with hlyAs (transporter gene) in preparation for secretion from the cell.

Depositor

Lingchong You

Publication

Pai et al Proc Natl Acad Sci U S A. 2012 Nov 27;10

Inserts

BlaMs
hlyAs

Promoter

p(lac/ara-1)

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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Thy1.2-Roxed-Cre

Plasmid

#51276

Purpose

Expression of the Dre-dependent Roxed-Cre gene under the control of the Thy1.2 expression cassette

Depositor

Pawel Pelczar

Publication

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

Roxed-Cre

Promoter

Thy1.2

Available since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pICH41276

Plasmid

#47994

Depositor

Sylvestre Marillonnet

Publication

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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GfaABC1D-fDIO-mNeonGreen

Plasmid

#241240

Purpose

FRT-dependent expression of fluorescent protein, mNeonGreen, in astrocytes

Depositor

Jun Nagai

Publication

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

fDIO-mNeonGreen

Use

AAV

Available since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-luxOFP

Plasmid

#229950

Purpose

Mammalian expression of a de novo designed, FRET-based neoLux1.2-CyOFP1 fusion bioluminescent probe (max. Em=588)

Depositor

Andy Yeh

Publication

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxOFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pACRISPR-TP

Plasmid

#203589

Purpose

A sgRNA expression plasmid for genome editing in Burkholderia

Depositor

Leonilde Moreira

Publication

Ferreira et al Appl Environ Microbiol. 2024 Feb 21;90(2

Type

Empty backbone

Expression

Bacterial

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-gfaABC1D-R-diLACCO1

Plasmid

#208105

Purpose

Control biosensor for intracellular L-lactate

Depositor

Robert Campbell, Marie-Eve Paquet

Publication

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

R-iLACCO1.1

Use

AAV

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDuex2-rsFusionRed1

Plasmid

#205758

Purpose

Expression of rsFusionRed1 in mamalian cells

Depositor

Edward Boyden

Publication

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rsFursionRed1

Expression

Mammalian

Mutation

wt

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pHG7

Plasmid

#188255

Purpose

Integrating plasmid contains the C31 int/attP locus and the hygromycin-resistance gene for selection

Depositor

Hong Gao, Maggie Smith

Publication

Gao et al Access Microbiol. 2021 Dec 8;3(12):00029

Type

Empty backbone

Expression

Bacterial

Promoter

act1p/actII-orf4

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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NK804

Plasmid

#166058

Purpose

Puromycin resistance and MRLC-mTFP expression for Salpingoeca rosetta

Depositor

Nicole King

Publication

Brunet et al Elife. 2021 Jan 15;10. pii: 61037. doi:

Insert

MRLC-mTFP

Use

S. rosetta expression

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ143-ZmUbi

Plasmid

#138107

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs driven by Zea mays Ubi promoter

Depositor

Yiping Qi

Publication

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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p33Lb

Plasmid

#158156

Purpose

Expression of crRNA array and LbCas12a using one Pol II promoter. For plant genome editing.

Depositor

Kabin Xie

Publication

Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pRGEB33

Plasmid

#158151

Purpose

Construction of inPTG-Cas9 plasmids expressing gRNA within an engineered intron for binary vector plant genome editing.

Depositor

Kabin Xie

Publication

Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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1xNLS-pMJ915v2

Plasmid

#88915

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Publication

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

Cas9

Use

CRISPR

Expression

Bacterial

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRVV210

Plasmid

#87628

Purpose

Drives Bxb1 expression in the D. melanogaster germ line.

Depositor

Richard Mann

Publication

Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

Insert

Bxb1

Expression

Insect

Promoter

vasa

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRVV601

Plasmid

#87632

Purpose

RMCE Bxb1 attB-flanked vector.

Depositor

Richard Mann

Publication

Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

Insert

Bxb1 attB

Use

Rmce

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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