We narrowed to 58,607 results for: plasmid
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Plasmid#164222PurposeExpresses soluble , dimeric Fc-His fusion protein for the human ACE2 extracellular domain, along with dTomato reporterDepositorAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only
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LentiCRISPR v2-sgLKB1 clone1
Plasmid#162125PurposeLentiviral sgRNA plasmid targeting human LKB1DepositorInsertsgLKB1 (STK11 Human)
UseLentiviralAvailable SinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-vCre (AAV Retrograde)
Viral Prep#55638-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-EF1a-vCre (#55638). In addition to the viral particles, you will also receive purified pAAV-EF1a-vCre plasmid DNA. EF1a-driven expression of VCre. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-trGluc
Plasmid#158959PurposeDNA donor plasmid containing repair template for Gaussia luciferase (Gluc) of bioluminescent repair reporter (BLRR).DepositorInserttrGluc
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNBU2_erm_SIE1
Plasmid#136356PurposeSelection by Inducible Effectors Test PlasmidDepositorInsertsType VI effector bfe1
Type VI effector bte1
BT_4676
tetR
UseUnspecifiedMutationATG removed to add signal sequence before geneAvailable SinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-EYFP (AAV PHP.V1)
Viral Prep#104052-AAVPHP.V1PurposeReady-to-use AAV PHP.V1 particles produced from pAAV-CAG-DIO-EYFP (#104052). In addition to the viral particles, you will also receive purified pAAV-CAG-DIO-EYFP plasmid DNA. CAG-driven, Cre-dependent expression of EYFP. These AAV were produced with the PHP.V1 serotype, which permits efficient transduction of brain vascular cells. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsEYFP (Cre-dependent)Available SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCgCas9_recT
Plasmid#153337PurposeDouble-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; KanrDepositorInsertCas9
UseCRISPRExpressionBacterialAvailable SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT-ADARcd-E488Q-V5
Plasmid#139686PurposeExpresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5DepositorAvailable SinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA
Plasmid#134925PurposeComponents for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.DepositorInsertCascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
ExpressionMammalianPromoterEF1aAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA
Plasmid#134922PurposeComponents for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.DepositorInsertCascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
ExpressionMammalianPromoterEF1aAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP7b-WPRE (AAV Retrograde)
Viral Prep#104489-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pGP-AAV-syn-jGCaMP7b-WPRE (#104489). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7b-WPRE plasmid DNA. Syn-driven jGCaMP7b calcium sensor. jGCaMP7b exhibits the brightest resting fluorescence of the jGCaMP7 variants and can be used for imaging of small neuronal processes. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti_AID-Dam-lamin B1
Plasmid#119764PurposeLentiviral plasmid for Mammalian ExpressionDepositorInsertAID-Dam-lamin B1
UseLentiviralExpressionMammalianAvailable SinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC1749
Plasmid#127983PurposeAMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertCpf1
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationcodon optimized for Aspergillus nidulansPromoterAspergillus nidulans tef1 promoterAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
FRP2371
Plasmid#127577PurposeRepressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, His3MX selection markerDepositorInsertPRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
UseSynthetic Biology; Shuttle vectorExpressionYeastAvailable SinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
amilCP_Pink chromoprotein
Plasmid#117851PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses amilCP_Pink chromoprotein in E. coliDepositorInsertpromoter, RBS, amilCP_Pink
UseSynthetic Biology; Escherichia coliMutation64A,65C, BioBrick sites removedAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
cjBlue chromoprotein
Plasmid#117844PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses cjBlue chromoprotein in E. coliDepositorInsertpromoter, RBS, cjBlue
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-OptoTGFBRs
Plasmid#118942PurposeMammalian expression plasmid of OptoTGFBRs (optogenetically-activated TGFb receptors)DepositorTagstdTomatoExpressionMammalianPromoterCMVAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMTMVi-M
Plasmid#118757PurposeIntermediate plasmid to build tobacco mosaic virus recombinant clones that move systemically throughout the plant.DepositorInsertTobacco mosaic virus cDNA fragment with a cloning polylinker
UseIntermediate to build tobacco mosaic virus recomb…Available SinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
hSUMO1 GFP
Plasmid#118360PurposeThis plasmid expresses the full length human SUMO1.DepositorAvailable SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pV1393
Plasmid#111430PurposeCaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)DepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRExpressionYeastAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only