We narrowed to 56,182 results for: plasmid

1. pDCC5

   Plasmid

   #59984

   Purpose

   Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassette

   Depositor

   Peter Duchek

   Article

   Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

   Insert

   Cas9-D10A
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Insect

   Mutation

   D10A

   Promoter

   hsp70Bb

   Available Since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGW-GFP-GOI-TurboID

   Plasmid

   #209382

   Purpose

   Gateway compatible plasmid for TurboID-based proximity labelling experiment in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Type

   Empty backbone

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Available Since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJAT57

   Plasmid

   #204303

   Purpose

   Expresses Cas9 under UAS control. HDR integrant markers with DsRed expressed in flight muscles.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   MHC-DsRed
   

   Use

   CRISPR

   Promoter

   MHC

   Available Since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBEVY-GA

   Plasmid

   #51227

   Purpose

   bi-directional expression vector for yeast, galactose inducible, ADE2 selection

   Depositor

   Charles Miller

   Article

   Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

   Type

   Empty backbone

   Expression

   Yeast

   Promoter

   GAL1,10

   Available Since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pK3FS

   Plasmid

   #85776

   Purpose

   N-ICE plasmid pK3FS

   Depositor

   Scott Briggs

   Article

   Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

   Insert

   loxP-KanMX-loxP-3FLAG
   

   Use

   Cre/Lox

   Expression

   Yeast

   Available Since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUdO4k

   Plasmid

   #210237

   Purpose

   low-copy plasmid for bacterial expression

   Depositor

   François-Xavier Campbell-Valois

   Article

   Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. CD44I-c013

   Plasmid

   #175450

   Purpose

   Protein expression/secretion in mammalian cells. CD44 Link module (exons 2-5), A20-P220.

   Depositor

   Opher Gileadi

   Insert

   CD44 (CD44 Human)
   

   Tags

   His6

   Expression

   Mammalian

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pH3F-GAL1

   Plasmid

   #85775

   Purpose

   N-ICE plasmid pH3F-GAL1

   Depositor

   Scott Briggs

   Article

   Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

   Insert

   loxP-HphMX-loxP-GAL1p-3FLAG
   

   Use

   Cre/Lox

   Expression

   Yeast

   Available Since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGBW-m4092694

   Plasmid

   #153907

   Purpose

   Bacterial expression plasmid for fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | t7 consensus from NEB, G is +1

   Available Since

   June 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. JARID1BA

    Plasmid

    #74646

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    JARID1BA (KDM5B Human)
    

    Use

    Baculovirus

    Tags

    His-6-TEV

    Mutation

    Leu101-(Gly)4-Ala374 deletion mutant

    Promoter

    PH

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pLenti HsATP10B_WT-T2A-His-flag-TMEM30A variant1

    Plasmid

    #191675

    Purpose

    transfer plasmid for lentiviral vector production expressing Hs ATP10B WT and His/Flag tagged Hs TMEM30A

    Depositor

    Veerle Baekelandt

    Inserts

    ATP10B (ATP10B Human)
    TMEM30A (TMEM30A Human)
    

    Use

    Lentiviral

    Tags

    6X his and flag

    Available Since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGBW-m4092709

    Plasmid

    #153859

    Purpose

    Bacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Bacterial

    Mutation

    Nearly native encoding;handful SNPs as watermarks…

    Promoter

    Promoter | t7 consensus from NEB, G is +1

    Available Since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pJ211_WT_hi

    Plasmid

    #128850

    Purpose

    High copy number plasmid for expression of E. coli flagellin from its natural promoter

    Depositor

    Mark Pallen

    Article

    Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

    Insert

    Flagellin
    

    Expression

    Bacterial

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGBW-m4046538

    Plasmid

    #149128

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available Since

    May 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pK3F

    Plasmid

    #85770

    Purpose

    N-ICE plasmid pK3F

    Depositor

    Scott Briggs

    Article

    Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

    Insert

    loxP-KanMX-loxP-3FLAG
    

    Use

    Cre/Lox

    Expression

    Yeast

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pH3FS-GAL1

    Plasmid

    #85782

    Purpose

    N-ICE plasmid pH3FS-GAL1

    Depositor

    Scott Briggs

    Article

    Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

    Insert

    loxP-HphMX-loxP-GAL1p-3FLAG
    

    Use

    Cre/Lox

    Expression

    Yeast

    Available Since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGBW-m4137387

    Plasmid

    #149544

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Human, Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    wt;19-aa Ctrunc

    Promoter

    CMV

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pUdO4t

    Plasmid

    #210249

    Purpose

    low-copy plasmid for bacterial expression

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGBW-m4046494

    Plasmid

    #149137

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 surface glycoprotein (S Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pK3FS-CYC1

    Plasmid

    #85779

    Purpose

    N-ICE plasmid pK3FS-CYC1

    Depositor

    Scott Briggs

    Article

    Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

    Insert

    loxP-KanMX-loxP-CYC1p-3FLAG
    

    Use

    Cre/Lox

    Expression

    Yeast

    Available Since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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