We narrowed to 17,911 results for: por
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Plasmid#80426PurposeUsed for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused to Cdt1 peptide and is degraded during S/G2 phases of the cell-cycle.DepositorInsertSpCas9-Cdt1
UseCRISPRTagsCdt1Mutationcodon optimized for humanAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
FKBP-epsin(144-575)-GFP
Plasmid#100733PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosisDepositorInsertepn1 (Epn1 Mouse)
TagsFKBP and GFPExpressionMammalianMutationclathrin binding middle part aa 144-575PromoterCMVAvailable SinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEasyG2_hph
Plasmid#184915PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_hph recombines in vivo with a PCR product from pEasyG2_mic.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWPI-UPP1-3xFLAG
Plasmid#201644PurposeOver-expression of 3xFLAG-tagged human UPP1DepositorAvailable SinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBacM13-8His-ZZ-LTLT-Hook3-SNAPf
Plasmid#130976PurposeExpression of 8His-ZZ-LTLT tagged Hook3-SNAPf in insect cells for protein purification.DepositorAvailable SinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T5-sfGFP*- MmH-MH4R
Plasmid#196409PurposeExpress sfGFP with TAG and enzymes for biosynthesizing DOPADepositorInsertsExpressionBacterialAvailable SinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
nuc-GafD-mTurboID-HA
Plasmid#184641PurposeExpresses 3xHA-tagged GlycoID construct: GafD_short-miniTurboID in mammalian nucleus for O-GlcNAc interactome taggingDepositorInsertnuc-GlycoID-HA
Tags3xHA tag and Nuclear localization sequenceExpressionMammalianMutationGafD -> expressed 22-178 ; BirA* -> express…PromoterCMVAvailable SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/MPL-V5
Plasmid#204531PurposeMammalian expression of human MPL-V5DepositorInsertMPL-V5 (MPL Human)
ExpressionMammalianAvailable SinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRE-UBC-mCD24-T2A-GFP
Plasmid#205448Purposelentiviral plasmid for expression of mouse CD24DepositorAvailable SinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
LUC05 (L/Cys/P)
Plasmid#112146PurposeMammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activityDepositorInsertLuciferase coding region with wild type Cysteine 258 and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Photinus pyralis, Rat)
ExpressionMammalianMutationnoneAvailable SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-KPNA2 zeo
Plasmid#192330PurposeLentiviral expression vector for an inducible 3xFLAG-KPNA2DepositorAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSS1 (hLPL-FLAG)
Plasmid#102447PurposeExpresses FLAG-tagged human LPL in mammalian cellsDepositorAvailable SinceMay 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast CHKA
Plasmid#218540Purposehuman CHKA cDNADepositorAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKT2/CAGXSP/PalmReNL
Plasmid#182970PurposeStably express PalmReNL in mammalian cells via the sleeping beauty transposon system.DepositorInsertPalmReNL
ExpressionMammalianMutationN/AAvailable SinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
MYH11-gRNA1
Plasmid#132587PurposegRNA used for knockin NanoLuc and tdTomado (separated by 2A) into MYH11 allele (MYH11-NanoLuc-2A-tdTomato vector) )DepositorInsertMYH11-gRNA1
ExpressionBacterialAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-mCar-EGFP-WPRE-BGH-pA-UBC-Bla
Plasmid#174620PurposePiggyBac plasmid expressing EGFP under the control of a cone arrestin promoterDepositorInsertenhanced green fluorescent protein
UsePiggybacExpressionMammalianAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV2_hSyn_NES-Caprola_on-mEGFP_WRPE-SV40
Plasmid#194691PurposehSyn1 driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression through AAV transductionDepositorInsertCaprola_on-mEGFP
UseAAVTagsmEGFPPromoterhSyn1Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MCU-D261A
Plasmid#31729DepositorAvailable SinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
PGK EGFP-PPM1H-WT
Plasmid#200084PurposeMammalian expression of wild-type PPM1HDepositorAvailable SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/sh-Col1a1/GFP4_Seq1.2
Plasmid#201403PurposeKnockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertCOL1A1
ExpressionMammalianAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only