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pWZ414-F14

Plasmid

#23063

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 deleted amino acids 3-29

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
pAAV-hSynI-mCherry-F2A-TVA-T2A-RabG-WPRE-hGH poly (A) (Alex_03)

Plasmid

#71654

Purpose

AAV expression of mCherry, TVA and RabG in human and mouse neurons for rabies mono-synaptic transfer experiments

Depositor

Mirjana Maletić-Savatić

Insert

F2A-TVA-T2A-RabG

Use

AAV; Rabies virus

Expression

Mammalian

Promoter

hSynI

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pBbA5k-AcrB/AcrD/AcrFa, ABO_0964

Plasmid

#86202

Purpose

Expresses efflux pump in E. coli to provide resistance to biofuel (medium copy)

Depositor

Mary Dunlop

Article

Turner et al ACS Synth Biol. 2015 Oct 16;4(10):1056-6

Insert

Efflux pump

Expression

Bacterial

Promoter

ATCGTTTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA…

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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TM SE T206A

Plasmid

#79664

Purpose

More dimeric SE attached to Triple Mutant GEVI from Piao et al 2015

Depositor

Bradley Baker

Article

Kang et al Sci Rep. 2016 Apr 4;6:23865. doi: 10.103

Insert

Triple Mutant SE T206A

Expression

Mammalian

Mutation

T206A

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pJK593

Plasmid

#71587

Purpose

Produces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) with N-terminal His6 tag (H6YfdE)

Depositor

T. J. Kappock

Article

Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:

Insert

acetyl-CoA:oxalate CoA-transferase (yfdE )

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pGolt1-mCherry

Plasmid

#73361

Purpose

In vivo protein protein interaction studies/ expression in mammalian cells

Depositor

Michael Kreutz

Insert

TMD (trans-membrane domain) of Calneuron-2 (Cabp7 Rat)

Tags

mCherry

Expression

Mammalian

Promoter

CMV immediate early promoter

Available Since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pGolt2-mCherry

Plasmid

#73358

Purpose

In vivo protein protein interaction studies/ expression in mammalian cells

Depositor

Michael Kreutz

Insert

TMD (trans-membrane domain) of Calneuron-2 (Cabp7 Rat)

Tags

mCherry

Expression

Mammalian

Promoter

CMV immediate early promoter

Available Since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pJK594

Plasmid

#71588

Purpose

Produces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) with C-terminal His6 tag (YfdEH6)

Depositor

T. J. Kappock

Article

Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:

Insert

acetyl-CoA:oxalate CoA-transferase (yfdE )

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pJK349

Plasmid

#71566

Purpose

Produces Helianthus annuus delta12 fatty acid acetylenase (vFAD2) in yeast

Depositor

T. J. Kappock

Article

Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007

Insert

delta12 fatty acid acetylenase

Expression

Bacterial and Yeast

Promoter

GAL1

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pUC19_extendedTTE1564

Plasmid

#60999

Purpose

T7-transcription template for Tte mRNA used in SiM-KARTS

Depositor

Nils Walter

Article

Rinaldi et al Nat Commun. 2016 Jan 19;7:8976. doi: 10.

Insert

TTE1564 transcript

Use

Unspecified

Promoter

T7

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pMZ036

Plasmid

#66973

Purpose

Expresses zebrafish Chk1

Depositor

Michael Lampson

Article

Zhang et al Cell Cycle. 2014;13(24):3828-38. doi: 10

Insert

zebrafish Chk1

Use

Zebrafish mrna injection

Expression

Mammalian

Mutation

Q341H mutation compared to GenBank reference sequ…

Promoter

CMV

Available Since

Jan. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pJK272

Plasmid

#71562

Purpose

Produces Crepis alpina delta12 fatty acid acetylenase (vFAD2) in yeast

Depositor

T. J. Kappock

Article

Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007

Insert

delta12 fatty acid acetylenase

Expression

Bacterial and Yeast

Promoter

GAL1

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pMZ039

Plasmid

#67757

Purpose

Expresses N-terminal kinase domain of Chk1 with C-term GFP fluorescent tag

Depositor

Michael Lampson

Article

Zhang et al Cell Cycle. 2014;13(24):3828-38. doi: 10

Insert

zebrafish Chk1

Tags

GFP

Mutation

only encodes N-term kinase domain (amino acids 1-…

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pJexpress414_OmpA171_R4cons_Y129H

Plasmid

#64302

Purpose

Conservative version of OmpA Redesign 4 with Y129H mutation

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_R4cons_Y129H

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Mutation

25 mutations to lipid-facing surface

Promoter

T7

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only
pUC19 gene 60 to gap

Plasmid

#49158

Purpose

Template for generating gene 60 mRNA, sequence 5' of coding gap only

Depositor

Nils Walter

Article

Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

Insert

Bacteriophage T4 gene 60

Mutation

Coding gap and downstream sequence have been del…

Promoter

T7

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-Myotilin-C-14

Plasmid

#55316

Purpose

Localization: Cytoskeleton, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Myotilin (MYOT Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pBbA5k-EPL12

Plasmid

#45404

Depositor

Aindrila Mukhopadhyay

Article

Dunlop et al Mol Syst Biol. 2011 May 10;7:487. doi: 1

Insert

PP_3456

Use

Synthetic Biology

Tags

His

Promoter

LacUV5

Available Since

Jan. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pGGB005

Plasmid

#48822

Purpose

Provides the SV40 nuclear localization signal as GreenGate N-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

SV40 nuclear localization signal

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGA003

Plasmid

#48814

Purpose

Provides the WUS promoter as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

WUS promoter

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pDR-AmTrac-GS

Plasmid

#47769

Purpose

expresses AmTrac-GS in yeast

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

AmTrac-GS

Tags

mcpGFP

Expression

Yeast

Promoter

PMA

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only