We narrowed to 19,051 results for: Tec

1. non-targeting control gRNA (BRDN0001149423)

   Plasmid

   #80255

   Purpose

   3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   Non-targeting control gRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. Agarabi CHO Light Chain in pJ201

   Plasmid

   #111566

   Purpose

   Cloning vector containing CDS for the murine light chain of the model chimeric mouse-human IgG1 expressed by the Agarabi CHO cell line

   Depositor

   Cyrus Agarabi

   Article

   Velugula-Yellela et al Biotechnol Prog. 2018 Jan;34(1):262-270.

   Insert

   Murine light chain
   

   Use

   Synthetic Biology; Cloning vector

   Promoter

   None

   Available Since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. PFKFB4 gRNA (BRDN0001149495)

   Plasmid

   #77123

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PFKFB4

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PFKFB4 (PFKFB4 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28b-BE3∆UGI

   Plasmid

   #96943

   Purpose

   The plasmid encoding the His6-rAPOBEC1-XTEN-nCas9 protein (BE3∆UGI) was generated by site-directed mutagenesis using pET28b-BE1 (Addgene plasmid #73018).

   Depositor

   Daesik Kim

   Article

   Kim et al Nat Biotechnol. 2017 May;35(5):475-480.

   Insert

   BE3∆UGI
   

   Tags

   His6 tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. STK17A gRNA (BRDN0001149480)

   Plasmid

   #77916

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human STK17A

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   STK17A (STK17A Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. STK17B gRNA (BRDN0001146764)

   Plasmid

   #76798

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human STK17B

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   STK17B (STK17B Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. p3s-ABE7.10

   Plasmid

   #113128

   Purpose

   expressing ABE7.10 (TadA-TadA*7.10-nCas9-NLS-HA), human codon optimized

   Depositor

   Jin-Soo Kim

   Article

   Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.

   Insert

   ABE 7.10
   

   Tags

   HA epitope and SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. PKN3 gRNA (BRDN0001146792)

   Plasmid

   #77948

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PKN3

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PKN3 (PKN3 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. PKN3 gRNA (BRDN0001146747)

   Plasmid

   #77949

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PKN3

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PKN3 (PKN3 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. PKN3 gRNA (BRDN0001487150)

    Plasmid

    #77950

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PKN3

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PKN3 (PKN3 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAd5-Blue

    Plasmid

    #174431

    Purpose

    pAd5-Blue provides a platform for the rapid construction of recombinant and replication defective Adenovirus. This vector contains unique restriction enzyme sites to allow cloning of a gene.

    Depositor

    Teresa De Los Santos

    Article

    Fernandez-Sainz et al Virology. 2017 Feb;502:123-132. doi: 10.

    Insert

    β-galactosidase α gene fragment
    

    Use

    Adenoviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA_B2AR-no-pep

    Plasmid

    #47439

    Purpose

    control FRET sensor for beta2-adrenergic receptor

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

    Insert

    beta2-adrenergic receptor (ADRB2 Human)
    

    Tags

    10 nm ER/K helix, HA, mCerulean, mCitrine, and no…

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    Sept. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

13. A*03:02:01

    Plasmid

    #203294

    Purpose

    Overexpress HLA allele

    Depositor

    Derin Keskin, Catherine Wu

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*03:02:01 (HLA-A Human)
    

    Expression

    Mammalian

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti myc-NL-GW

    Plasmid

    #113454

    Purpose

    Lentiviral NanoLuc Gateway shuttle vector for N-terminal fusions

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Type

    Empty backbone

    Use

    Lentiviral and Luciferase

    Tags

    cmyc-NanoLuc

    Expression

    Mammalian

    Promoter

    hUbC

    Available Since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-TRE3G-BE3-PGK-Puro

    Plasmid

    #110845

    Purpose

    Lentiviral vector for dox-inducible expression of BE3 (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3
    

    Use

    Lentiviral

    Mutation

    D10A

    Promoter

    TRE3G

    Available Since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-Flag-LmoCas6

    Plasmid

    #126485

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas6
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. shMEK1/2 v2 neo

    Plasmid

    #72570

    Purpose

    Hairpin targeting MEK1 and MEK2 (murine).

    Depositor

    Kevin Janes

    Article

    Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

    Insert

    MEK1/2 (Map2k1, Map2k2 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDEST_FLAG-HUWE1_UBM-mut

    Plasmid

    #187150

    Purpose

    mammalian cell expression of FLAG-HUWE1 L2976A/P2977A

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    L2976A/P2977A

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST_FLAG-HUWE1_UIM-mut

    Plasmid

    #187148

    Purpose

    mammalian cell expression of FLAG-HUWE1 A1376G/S1382A

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    A1376G/S1382A

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST_FLAG-HUWE1_UBA-mut

    Plasmid

    #187146

    Purpose

    mammalian cell expression of FLAG-HUWE1 M1328A/F1330A

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    M1328A/F1330A

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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