161,621 results

1. Ii-Str_ManII-SBP-tagBFP

   Plasmid

   #65257

   Purpose

   synchronize trafficking of ManII from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Ii-Streptavidin and truncated Mannosidase II
   

   Use

   Tags

   Streptavidin Binding Protein (SBP) and tagBFP

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 116 of ManII

   Promoter

   CMV

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19/mChe-PM

   Plasmid

   #183168

   Purpose

   Construct for transient expression of plasma membrane-targeted mCherry in plants.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Insert

   mCherry-PM
   

   Use

   Tags

   OsRac3, O. sativa rac-like GTP-binding protein 3

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR-L4R1-CAG

   Plasmid

   #139522

   Purpose

   Provides constitutive promoter between attL4-attR1 cassette on Gateway entry vector

   Depositor

   Minoru Ko

   Insert

   CAG promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDestTol2CG2-U6:gRNA

   Plasmid

   #63156

   Purpose

   Destination vector for Gateway based on vector #395 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter, and cmlc2:GFP as a transgenesis marker.

   Depositor

   Leonard Zon

   Article

   Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

   Type

   Empty backbone

   Use

   CRISPR; Tol2 destination vector for gateway

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-TET-ZH-lox66FRT

   Plasmid

   #139563

   Purpose

   piggyBac expression vector with TET-ON system and Zeocin selection

   Depositor

   Minoru Ko

   Insert

   PGK-ZeoR-pA cassette, inserted in PB-TET vector
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-TET-BH-lox66FRT

   Plasmid

   #139562

   Purpose

   piggyBac expression vector with TET-ON system and Blasticidin selection

   Depositor

   Minoru Ko

   Insert

   PGK-BSD-pA cassette, inserted in PB-TET vector
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF0074

   Plasmid

   #142144

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   BACH2 (BACH2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. QPM-sgR (pTaU3)

   Plasmid

   #140448

   Purpose

   For sgRNA expression in monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

   Insert

   TaU3-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   TaU3

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. PCDNA3-GFP1-9 T2A mCherry

   Plasmid

   #124430

   Purpose

   Expresses GFP1-9 and T2A mCherry in mammalian cells

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   GFP1-9 T2A mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX6P1-mCherry-Nanobody

    Plasmid

    #70696

    Purpose

    Expresses GST-tagged anti-mCherry Nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

    Insert

    mCherry-Nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTP2620

    Plasmid

    #113311

    Purpose

    human Rad50 in baculovirus transfer vector

    Depositor

    Tanya Paull

    Article

    Lee et al J Biol Chem. 2013 May 3;288(18):12840-51

    Insert

    Rad50 (RAD50 Human)
    

    Use

    Tags

    HA, 6xhis

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-SpRY-HF1-ABE8e

    Plasmid

    #185670

    Purpose

    Expresses SpRY-HF1-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-nSpRY-HF1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    SpRY-HF1 D10A

    Promoter

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHyo245

    Plasmid

    #173147

    Purpose

    Expresses pheS_A294G by dual T7 promoter in e.coli

    Depositor

    Seokhee Kim

    Article

    Park et al Nucleic Acids Res. 2021 Apr 6;49(6):e32.

    Insert

    pheS_A294G
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTP813

    Plasmid

    #113308

    Purpose

    human Mre11 in baculovirus transfer vector

    Depositor

    Tanya Paull

    Article

    Bhaskara et al Mol Cell. 2007 Mar 9;25(5):647-61. doi:

    Insert

    Mre11 (MRE11 Human)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Mtp-p53-ECFP

    Plasmid

    #53961

    Purpose

    Expresses CFP tagged p53 in mammalian cells

    Depositor

    Galit Lahav, Uri Alon

    Article

    Lahav et al Nat Genet. 2004 Feb;36(2):147-50. Epub 2

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    metallothionein promoter

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. MaLionR

    Plasmid

    #113908

    Purpose

    Red ATP sensor

    Depositor

    Tetsuya Kitaguchi

    Article

    Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

    Insert

    MaLionR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. PMXS-NDI1

    Plasmid

    #72876

    Purpose

    expresses NDI1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Birsoy et al Nature. 2014 Apr 3;508(7494):108-12. doi

    Insert

    NDI1 (NDI1 Budding Yeast)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. AiP1706 - pAAV-AiE2402m-minBG-SYFP2-WPRE-BGHpA

    Plasmid

    #214522

    Purpose

    AiE2402m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Bosiljka Tasic, The Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYY10-2µ-TP901

    Plasmid

    #235771

    Purpose

    2µ plasmid for constitutive expression of TP901 under RNR2 promoter.

    Depositor

    Chang Liu

    Article

    Pisera et al bioRxiv [Preprint]. 2024 Nov 30:2024.11.

    Insert

    TP901 integrase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYY13-URA3-TP901LP

    Plasmid

    #235772

    Purpose

    Plamids for generation of donor DNA for construction of a TP901-compatible landing pad p1 with URA3 selection marker.

    Depositor

    Chang Liu

    Article

    Pisera et al bioRxiv [Preprint]. 2024 Nov 30:2024.11.

    Insert

    URA3
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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