We narrowed to 10,808 results for: T7
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Plasmid#127264PurposeFor in vitro translation of human ELL2 with Met133ILeu, Met186ILeuDepositorInsertELL2 (Met133ILeu, Met186ILeu) (ELL2 Human)
UseIn vitro translationMutationTwo Mets (133,186 )to ILeu QuikchangePromoterT7Available SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met331Leu ELL2
Plasmid#127262PurposeFor in vitro translation of human ELL2 with Met331 mutated to Ileu to ask if there is internal initiationDepositorInsertELL2 (Met331 to Ileu) (ELL2 Human)
UseIn vitro translationMutationMet331 to ILeu QuikchangePromoterT7Available SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met1ILeu ELL2
Plasmid#127258PurposeFor in vitro translation of human ELL2 with Met1 mutated to Ileu to ask if there is internal initiationDepositorInsertELL2 (Met1 to Ileu) (ELL2 Human)
UseIn vitro translationMutationMet1 to Ileu by QuikchangePromoterT7Available SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) K83A
Plasmid#125829PurposeExpresses GST-RPN13 (19-132) K83A in E.coliDepositorInsertRPN13 (ADRM1 Human)
TagsGSTExpressionBacterialMutationchanged lysine 83 to alaninePromoterT7Available SinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) K97D
Plasmid#125824PurposeExpresses GST-RPN13 (19-132) K97D in E.coliDepositorInsertRPN13 (ADRM1 Human)
TagsGSTExpressionBacterialMutationchanged lysine 97 to aspartatePromoterT7Available SinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) R104A
Plasmid#125826PurposeExpresses GST-RPN13 (19-132) R104A in E.coliDepositorInsertRPN13 (ADRM1 Human)
TagsGSTExpressionBacterialMutationchanged arginine 104 to alaninePromoterT7Available SinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) R104D
Plasmid#125827PurposeExpresses GST-RPN13 (19-132) R104D in E.coliDepositorInsertRPN13 (ADRM1 Human)
TagsGSTExpressionBacterialMutationchanged arginine 104 to aspartatePromoterT7Available SinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) R104E
Plasmid#125828PurposeExpresses GST-RPN13 (19-132) R104E in E.coliDepositorInsertRPN13 (ADRM1 Human)
TagsGSTExpressionBacterialMutationchanged arginine 104 to glutamatePromoterT7Available SinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJET1.2_Rab28_Sense
Plasmid#124441PurposePlasmid for sense in situ probe in vitro transcriptionDepositorAvailable SinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGluSnFR E25R
Plasmid#119831PurposeProduction of recombinant iGluSnFR E25R variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluSnFR E25R
TagsGSTExpressionBacterialMutationE25RPromoterT7Available SinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
N229A Lo-MetQ
Plasmid#118268PurposeN229A Mutation in Methionine Binding Protein (MetQ) used to solve crystal structureDepositorInsertMetQ (N229A) (metQ E. coli)
Tags10x His Tag and Enterokinase Cleavage SiteExpressionBacterialMutationChanged Asparagine (Asn) 229 to Alanine (Ala)PromoterT7 PromoterAvailable SinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTG-mTDGa.0
Plasmid#81048Purposebacterial expression of murine TDGDepositorAvailable SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(P46A)
Plasmid#112721PurposeThis mutation (P46A) marks the first residue within the evolved region of U1A.DepositorInsert6His-TEV-TBP6.7(P46A)
Tags6His-TEVExpressionBacterialMutationP46APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(Q48T)
Plasmid#112725PurposeThis mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.DepositorInsert6His-TEV-TBP6.7(Q48T)
Tags6His-TEVExpressionBacterialMutationQ48TPromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(P51A)
Plasmid#112728PurposeSimilar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.DepositorInsert6His-TEV-TBP6.7(P51A)
Tags6His-TEVExpressionBacterialMutationP51APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(Q54A)
Plasmid#112730PurposeAlthough not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.DepositorInsert6His-TEV-TBP6.7(Q54A)
Tags6His-TEVExpressionBacterialMutationQ54APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-C-SH2
Plasmid#111419Purposeexpress murine Syk (C)SH2 domain (His 162 to Gln 264), in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk (C)SH2 domain (His 162 to Gln 264), without any tag (Syk Mouse)
ExpressionBacterialPromoterT7 promoterAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-N-SH2
Plasmid#111274Purposeexpress murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
TagsHHHHHHENLYFQGExpressionBacterialPromoterT7 promoterAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a - Ec-ClpX D144L
Plasmid#111514PurposeExpresses E. coli ClpX with a C-terminal His tag and a mutation from D144 to L144DepositorInsertClpX
TagsHis tagExpressionBacterialMutationAspartatic acid 144 to LeucinePromoterT7Available SinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-dCys-T46C
Plasmid#110543PurposeBacterial expression of E.coli DHFR C85A/C152S/T46C mutantDepositorInsertecDHFR
ExpressionBacterialMutationT46 mutated to C46, C85 mutated to A85, C152 muta…PromoterT7Available SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only